基于电聚合没食子酸材料的氧氟沙星电化学传感器
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摘要
通过电聚合法一步制备了聚没食子酸材料修饰玻碳电极(Poly(GA)/GCE),构建了一种简单、灵敏的电化学传感器用于测定氧氟沙星(OFL).扫描电镜(SEM)图像研究表明,没食子酸聚合物成功修饰到玻碳电极表面.用电化学阻抗谱(EIS)和循环伏安法(CV)研究了电极的电化学性能,发现修饰电极阻抗减小并且对OFL的电流响应显著增强.在优化了电聚合循环次数、没食子酸浓度等电化学参数后,该传感器线性范围为1.0～100μmol·L~(-1),灵敏度为2 704,检出限为0.3μmol·L~(-1)(S/N=3).该传感器具有电流响应快、重复性好、稳定性好等优点,可用于药物和生物样品中OFL的测定.
A facile and sensitive electrochemical sensor is constructed for determination of ofloxacin(OFL) based on glassy carbon electrode(GCE) modified with poly(gallic acid)(Poly(GA)) by one step electro-polymerization.Gallic acid polymer is successfully modified on the surface of GCE observed by scanning electron microscopy(SEM).The electrochemical properties of the constructed sensor are characterized by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS).The modified electrode not merely exibits low impedance,but also enhances current response.After optimization of electrochemical parameters such as the number of polymerization cycles,polymer concentration and pH value,the proposed sensor has a linear range from 1.0 to 100 μmol·L~(-1),with a sensitivity of 2 704 and a detection limit of 0.3 μmol·L~(-1)(S/N=3).The developed sensor shows fast current responsibility,good reproducibility and stability.This prepared sensor can be successfully applied to the determination of OFL in pharmaceutical compounds and biological samples.
A facile and sensitive electrochemical sensor is constructed for determination of ofloxacin(OFL) based on glassy carbon electrode(GCE) modified with poly(gallic acid)(Poly(GA)) by one step electro-polymerization.Gallic acid polymer is successfully modified on the surface of GCE observed by scanning electron microscopy(SEM).The electrochemical properties of the constructed sensor are characterized by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS).The modified electrode not merely exibits low impedance,but also enhances current response.After optimization of electrochemical parameters such as the number of polymerization cycles,polymer concentration and pH value,the proposed sensor has a linear range from 1.0 to 100 μmol·L~(-1),with a sensitivity of 2 704 and a detection limit of 0.3 μmol·L~(-1)(S/N=3).The developed sensor shows fast current responsibility,good reproducibility and stability.This prepared sensor can be successfully applied to the determination of OFL in pharmaceutical compounds and biological samples.
引文
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[2] AWADALLAH B,SCHMIDT P C,WAHL M A.Quantitation of the enantiomers of ofloxacin by capillary electrophoresis in the parts per billion concentration range for in vitro drug absorption studies[J].Journal of Chromatography A,2003;988(1):135.
[3] BRVAR M,PERDIH A,HODNIK V,et al.In silico discovery and biophysical evaluation of novel 5-(2-hydroxybenzylidene) rhodanine inhibitors of DNA gyrase B[J].Bioorg Med Chem,2012,20(8):2572.
[4] REID G,POTTER P,DELANEY G,et al.Ofloxacin for the treatment of urinary tract infections and biofilms in spinal cord injury[J].International Journal of Antimicrobial Agents,2000,13(4):305.
[5] CHAN K P,CHU K O,LAI W W K,et al.Determination of ofloxacin and moxifloxacin and their penetration in human aqueous and vitreous humor by using high-performance liquid chromatography fluorescence detection[J].Analytical Biochemistry,2006,353(1):30.
[6] RODRIGUEZ E,MORENO-BONDI M C,MARAZUELA M D.Multiresidue determination of fluoroquinolone antimicrobials in baby foods by liquid chromatography[J].Food Chemistry,2011,127(3):1354.
[7] BYZOVA N A,SMIRNOVA N I,ZHERDEV A V,et al.Rapid immunochromatographic assay for ofloxacin in animal original foodstuffs using native antisera labeled by colloidal gold[J].Talanta,2014,119:125.
[8] HE K,BLANEY L.Systematic optimization of an SPE with HPLC-FLD method for fluoroquinolone detection in wastewater[J].Journal of Hazardous Materials,2015,282(4):96.
[9] SHAH P,PANDYA T,GOHEL M,et al.Development and validation of HPLC method for simultaneous estimation of rifampicin and ofloxacin using experimental design[J].Journal of Taibah University for Science,2018,13(1):146.
[10] ZHAO T,YU B F,TANG W J,et al.Spectrofluorimetric determination of ofloxacin in milk with N-(9-fluorenylmethyloxycarbonyl)-l-alanine[J].Spectrochimica Acta Part A,2015,148:125.
[11] EL-KOMMOS M E,SALEH G A,EL-GIZAWI S M,et al.Spectrofluorometric determination of certain quinolone antibacterials using metal chelation[J].Talanta,2003,60(5):1033.
[12] XIA Q H,YANG Y L,LIU M S.Aluminium sensitized spectrofluorimetric determination of fluoroquinolones in milk samples coupled with salting-out assisted liquid-liquid ultrasonic extraction[J].Spectrochimica Acta Part A,2012,96(10):358.
[13] ZHOU X T,WANG L M,SHEN G Q,et al.Colorimetric determination of ofloxacin using unmodified aptamers and the aggregation of gold nanoparticles[J].Mikrochim Acta,2018,185(7):355.
[14] SPRINGER V,JACKSéN J,EK P,et al.Capillary electrophoretic determination of fluoroquinolones in bovine milk followed by off-line maldi-tof-ms analysis[J].Chromatographia,2015,78(3/4):285.
[15] XIAO H T,FU X,LIANG S,et al.An approach to the determination of the enantiomeric excess at the extreme case by capillary electrophoresis[J].J Chromatogr A,2015,1408:250.
[16] HATAMLUYI B,LORESTANI @rGO nanocomposite decorated with poly(L-Cysteine) as a probe for simultaneous sensitive electrochemical determination of anticancer drugs,Ifosfamide and Etoposide[J].Biosens Bioelectron,2018,120:22.
[17] ZHANG Y,NIE J T,WEI H Y,et al.Electrochemical detection of nitrite ions using Ag/Cu/MWNT nanoclusters electrodeposited on a glassy carbon electrode[J].Sensors and Actuators B,2018,258:1107.
[18] WONG A,SILVA T A,VICENTINI F C,et al.Electrochemical sensor based on graphene oxide and ionic liquid for ofloxacin determination at nanomolar levels[J].Talanta,2016,161:333.
[19] ZHANG S,LIU Y J,LIN M H,et al.A dual amplified electrochemical immunosensor for ofloxacin:polypyrrole film-Au nanocluster as the matrix and multi-enzyme-antibody functionalized gold nanorod as the label[J].Electrochimica Acta,2013,90:246.
[20] HUANG K J,LIU X,XIE W Z,et al.Voltammetric behavior of ofloxacin and its determination using a multi-walled carbon nanotubes-Nafion film coated electrode[J].Microchimica Acta,2008,162(1/2):227.
[21] HE Z Y,ZANG S,LIU Y J,et al.A multi-walled carbon nanotubes-poly(L-lysine) modified enantioselective immunosensor for ofloxacin by using multi-enzyme-labeled gold nanoflower as signal enhancer[J].Biosens Bioelectron,2015,73:85.
[22] WU F H,XU F,CHEN L,et al.Cuprous oxide/nitrogen-doped graphene nanocomposites as electrochemical sensors for ofloxacin determination[J].Chemical Research in Chinese Universities,2016,32(3):468.
[23] JIANG Z M,LI G Y.A novel electrochemical sensor based on SH-beta-cyclodextrin functionalized gold nanoparticles/reduced-graphene oxide nanohybrids for ultrasensitive electrochemical sensing of acetaminophen and ofloxacin [J].International Journal of Electrochemical Science,2017,12(6):5157.
[24] SUN D F,LI H J,LI M J,et al.Electrodeposition synthesis of a NiO/CNT/PEDOT composite for simultaneous detection of dopamine,serotonin,and tryptophan[J].Sensors and Actuators B,2018,259:433.
[25] DONG S Q,BI Q,QIAO C D,et al.Electrochemical sensor for discrimination tyrosine enantiomers using graphene quantum dots and β-cyclodextrins composites[J].Talanta,2017,173:94.
[26] ARVAND M,SANAYEEI M,HEMMATI S,et al.Label-free electrochemical DNA biosensor for guanine and adenine by ds-DNA/poly(L-cysteine)/Fe3O4 nanoparticles-graphene oxide nanocomposite modified electrode[J].Biosens Bioelectron,2018,102:70.
[27] ZHONG M,YING T,PANG S F,et al.Pyrrole–phenylboronic acid:a novel monomer for dopamine recognition and detection based on imprinted electrochemical sensor[J].Biosensors & Bioelectronics,2015,64:212.
[28] GAJJALA R K R,PALATHEDATH S K.Cu@Pd core-shell nanostructures for highly sensitive and selective amperometric analysis of histamine[J].Biosensors & Bioelectronics,2018,102:242.
[29] PILEHVAR S,REINEMANN C,BOTTARI F,et al.A joint action of aptamers and gold nanoparticles chemically trapped on a glassy carbon support for the electrochemical sensing of ofloxacin[J].Sensors and Actuators B,2017,240:1024.
[30] SI X J,WEI Y L,WANG C L,et al.Sensitive electrochemical sensor for ofloxacin based on graphene/ zinc oxide composite film[J].Analytical Methods,2018,10(17):1961.
[31] 朱艳琼,杨光,韩宝三,等.碳纳米纤维负载金纳米粒子用于氧氟沙星的电分析检测[J].分子科学学报,2018,34(4):338.
[32] JIANG Z M,LIU Q,TANG Y R,et al.Electrochemical sensor based on a novel Pt-Au bimetallic nanoclusters decorated on reduced graphene oxide for sensitive detection of ofloxacin[J].Electroanalysis,2017,29(2):602.
[33] CHIGUTSA E,MEREDITH S,WIESNER L,et al.Population pharmacokinetics and pharmacodynamics of ofloxacin in South African patients with multidrug-resistant tuberculosis[J].Antimicrob Agents Chemother,2012,56(7):3857.