红水樱桃种子玻璃化超低温保存
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摘要
从含水量、装载时间、玻璃化时间和种壳4方面探究红水樱桃(Prunus pseudocerasu L. Hongshui)种子玻璃化超低温保存。结果显示,含水量以30%为好;装载以60%PVS_2在4℃下处理20 min为宜;玻璃化以PVS_2室温下处理60 min为宜;种壳决定保存效果,带种壳可获得100%成活率,而去种壳后几乎不萌发,污染严重。种子成活率检测用离体萌发法,经43℃水浴3 min解冻后接种到1/2 MS+1.0 mg/L BA+0.5 mg/L IBA+2.0 mg/L GA+3.0%蔗糖+0.7%琼脂粉培养基上。
引文
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[2]Reed B M,Sarasan V,Kane M,et al.Biodiversity conservation and conservation biotechnology tools[J].In Vitro Cell Dev Biol Plant,2011,47:1-4.
[3]Marcin M,Beata P P,Tadeusz T,et al.Desiccation tolerance and cryopreservation of seeds of black poplar (Populus nigra L.),a disappearing tree species in Europe[J].Eur J Forest Res,2015,134:53-60.
[4]Kanazawa Y,Xia K,Tan H Y,et al.Seed storage longevity of Hosta sieboldiana(Asparagaceae)[J].South African Journal of Botany,2015,98:6-9.
[5]程利红,段旭昌,张宗勤,等.中国红豆杉种子超低温保存技术研究[J].西北农林科技大学学报(自然科学版).2012,40(2):71-78.
[6]Chmielarz P.Cryopreservation of dormant orthodox seeds of forest trees:mazzard cherry(Prunus avium L.)[J].Ann.For.Sci,2009,66:405.
[7]Chmielarz P.Cryopreservation of conditionally dormant orthodox seeds of Betula pendula[J].Acta Physiol Plant,2010,32:591-596.
[8]Bernardo-Gil G,Oneto C,Antunes P,et al.Extraction of lipids from cherry seed oil using supercritical carbon dioxide[J].Eur.Food Res.Technol,2001,212:170-174.
[9]Crane J,Miller A L,Roekel J W,et al.Triacylglycerols determine the unusual storage physiology of Cuphea seed[J].Planta,2003,217:699-708.
[10]河英虎,傅伊倩,刘燕.合欢和青杄种子超低温保存研究[J].种子,2013,32(3):33-35,40.
[11]Engelmann F.Plant cryopreservation:Progress and Prospects[J].In Vitro Cellular & Developmental Biology- Plant,2004,40(5):427-433.
[12]刘燕,周慧,方标.园林花卉种子超低温保存研究[J].北京林业大学学报,2001,23(7):39-44.
[13]Benson E E.Cryopreservation of phytodiversity:a critical appraisal of theory and practice[J].Crit Rev Plant Sci,2008(27):141-219.
[14]Berjak P,Sershen,Varhese B,et al.Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic axes of recalcitrant-seeded species[J].Seed Science Research,2011,21:187-203.
[15]Engelmann F.Cryopreservation of embryos:an overview[J].Methods Mol Biol,2011(710):155-184.