淀粉细菌纤维素复合材料的制备及应用
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摘要
为了了解纤维素材料应用于实用性膳食纤维保健品中的作用,以淀粉和木醋杆菌活化菌种为原料,采取动静结合的发酵方式,制备出了淀粉/细菌纤维素复合材料(SBC)。采用离体实验模拟人体胃和肠道的pH环境,探讨了SBC在此环境之下的酸解效率,以及对于甘油和三聚氰胺的吸附能力和最大吸附量,为食用膳食纤维保健品的研发提供了依据。实验结果显示,SBC在酸性条件(模拟胃液,含量为0.15%的盐酸溶液)下的酸解效率为20.47%。甘油浓度为4%(V/V),三聚氰胺含量为4%(w/w)时,SBC的吸附效率最高。在此基础之上,SBC对甘油的最大吸附量为0.84 g(甘油)/1 g(SBC),对三聚氰胺的吸附量为9.92 mg(三聚氰胺)/1 g(SBC),且吸附量大约是纯细菌纤维素的2倍。
In order to understand the role of cellulosic materials in practical dietary fiber health products,starch/bacterial cellulose composites(SBC) were prepared by using starch and Acetobacter xylinum as the raw materials and adopting dynamic and static fermentation methods. In vitro experiments were carried out to simulate the pH environment of human stomach and intestine. The acid hydrolysis efficiency of SBC under this environment, as well as the adsorption capacity and maximum adsorption capacity for glycerol and melamine, were used as dietary fiber. Provide research and development basis for health products. The experimental results show that the acid hydrolysis efficiency of the SBC under acidic conditions(simulated gastric juice, 0.15% hydrochloric acid solution) is 20.47%. When the glycerin concentration is 4%(V/V) and the melamine content is 4%(w/w), the SBC has the highest adsorption efficiency. On this basis, the maximum adsorption capacity of SBC for glycerol is 0.84 g(glycerol)/1 g(SBC), and the adsorption amount of melamine is 9.92 mg(melamine)/1 g(SBC), and the adsorption amount is about twice that of pure bacterial cellulose.
In order to understand the role of cellulosic materials in practical dietary fiber health products,starch/bacterial cellulose composites(SBC) were prepared by using starch and Acetobacter xylinum as the raw materials and adopting dynamic and static fermentation methods. In vitro experiments were carried out to simulate the pH environment of human stomach and intestine. The acid hydrolysis efficiency of SBC under this environment, as well as the adsorption capacity and maximum adsorption capacity for glycerol and melamine, were used as dietary fiber. Provide research and development basis for health products. The experimental results show that the acid hydrolysis efficiency of the SBC under acidic conditions(simulated gastric juice, 0.15% hydrochloric acid solution) is 20.47%. When the glycerin concentration is 4%(V/V) and the melamine content is 4%(w/w), the SBC has the highest adsorption efficiency. On this basis, the maximum adsorption capacity of SBC for glycerol is 0.84 g(glycerol)/1 g(SBC), and the adsorption amount of melamine is 9.92 mg(melamine)/1 g(SBC), and the adsorption amount is about twice that of pure bacterial cellulose.
引文
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[13]邹瑜,陈仕艳,王静芸, et al.细菌纤维素吸附Cu2+的研究[J].材料科学与工程学报, 2008, 26(3):426-429.
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[15] Luo M, Li H, Huang C, et al. Cellulose-based absorbent production from bacterial cellulose and acrylic acid:Synthesis and performance[J]. Polymers, 2018, 10(7):702-719.
[2]李雷,汪东风,周小玲,等.茶叶多糖食品功能性研究[J].茶叶科学, 2006, 26(2):102-107.
[3]张宁,欧仕益,黄才欢.膳食纤维对有害元素吸附的研究[J].食品研究与开发, 2006, 27(8):163-166.
[4] Abral H, Anugrah A S, Hafizulhaq F, et al. Effect of nanofibers fraction on properties of the starch based biocomposite prepared in various ultrasonic powers[J]. International Journal of Biological Macromolecules, 2018, 116(3):1214-1221.
[5] Yang Y, Liu C, Chang P R, et al. Properties and structural characterization of oxidized starch/PVA/α-zirconium phosphate composites[J]. Journal of Applied Polymer Science, 2010, 115(2):1089-1097.
[6]陈亚非,赵谋明.水溶性与水不溶性膳食纤维对油脂、胆固醇和胆酸钠吸附作用研究[J].现代食品科技, 2005, 21(3):58-60.
[7] Panaitescu D M, Frone A N, Chiulan I, et al. Structural and morphological characterization of bacterial cellulose nano-reinforcements prepared by mechanical route[J]. Materials&Design, 2016:S0264127516311157.
[8] Ghanbari A, Tabarsa T, Ashori A, et al. Preparation and characterization of thermoplastic starch and cellulose nanofibers as green nanocomposites:Extrusion processing[J]. International Journal of Biological Macromolecules, 2018, 112(2):442-447.
[9]龚良玉,孙露霞.淀粉模板法辅助合成二氧化锰纳米棒及其电容特性研究[J].现代化工, 2010, 30(4):50-53.
[10] Woehl M A, Canestraro C D, Mikowski A, et al. Bionanocomposites of thermoplastic starch reinforced with bacterial cellulose nanofibres:Effect of enzymatic treatment on mechanical properties[J]. Carbohydrate Polymers, 2010, 80(3):866-873.
[11] Wan Y Z, Luo H, He F, et al. Mechanical, moisture absorption and biodegradation behaviours of bacterial cellulose fibre-reinforced starch biocomposites[J]. Composites Science and Technology, 2009, 69(7-8):1212-1217.
[12] Grande C J, Torres F G, Gomez C M, et al. Development of self-assembled bacterial cellulose-starch nanocomposites[J].Materials Science and Engineering:C, 2009, 29(4):1098-1104.
[13]邹瑜,陈仕艳,王静芸, et al.细菌纤维素吸附Cu2+的研究[J].材料科学与工程学报, 2008, 26(3):426-429.
[14] Martins I M G, Magina S P, Oliveira L, et al. New biocomposites based on thermoplastic starch and bacterial cellulose[J].Composites Science and Technology, 2009, 69(13):2163-2168.
[15] Luo M, Li H, Huang C, et al. Cellulose-based absorbent production from bacterial cellulose and acrylic acid:Synthesis and performance[J]. Polymers, 2018, 10(7):702-719.