摘要
黄粉虫可啮噬泡沫塑料,但是生物降解的效率较低。本文以黄粉虫为原料,运用差速离心法制取黄粉虫提取物,通过将聚苯乙烯泡沫塑料(EPS)小球浸泡于黄粉虫提取物的水溶液中并观察小球体积变化,证明黄粉虫提取物可以改变EPS的亲水性。为提升聚苯乙烯泡沫塑料分解效率,以乙酸乙酯和水的乳化液模仿黄粉虫肠道生化环境,探究黄粉虫提取物在该乳化液中对EPS的分解效率,通过质谱分析,证明分解效果良好。
引文
[1]Gerhardt PD,Lindgren DL.Penetration of packaging films:film materials used for food packaging tested for resistance to some common stored-product insects[J].California Agriculture,1954,8(6):3-4.
[2]Riudavets J,Salas I,Pons MJ.Damage characteristics produced by insect pests in packaging film[J].Journal of Stored Products Research,2007,43(4):564-570.
[3]杨军,宋怡玲,秦小燕.聚乙烯塑料的生物降解研究[J].环境科学,2007(05):1165-1168.
[4]沈叶红.黄粉虫肠道菌的分离和取食塑料现象的研究[D].华东师范大学,2011.
[5]Yang J,Yang Y,Wu WM,et al.Evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms[J].Environmental Science&Technology,2014,48(23):13776-13784.
[6]Ioakeimidis C,Fotopoulou KN,Karapanagioti HK,et al.The degradation potential of PET bottles in the marine environment:An ATR-FTIR based approach[J].Scientific Reports,2016,6:23501.
[7]Yoshida,S.;Hiraga,K.;Takehana,T.;Taniguchi,I.;Yamaji,H.;Maeda,Y.;Toyohara,K.;Miyamoto,K.;Kimura,Y.;Oda,K.(11 March 2016)."A bacterium that degrades and assimilates poly(ethylene terephthalate)".Science.351(6278):1196.
[8]陈冠舟,张白鹭,纪梦梦,吴晓刚,周君仪,陈家楠,王芸,田浩,张晓君.高通量测序探究啮食聚苯乙烯泡沫塑料黄粉虫的肠道菌群结构[J].微生物学通报,2017,44(09):2011-2018.
[9]Austin,H.P.,Allen,M.D.,Donohoe,B.S.,Rorrer,N.A.,Kearns,F.L.,&Silveira,R.L.,et al.(2018).Characterization and engineering of a plastic-degrading aromatic polyesterase.Proceedings of the National Academy of Sciences of the United States of America,115(19),E4350.
[10]孔芳,洪康进,徐航,赵世光,王幼平.基于啮食泡沫塑料黄粉虫肠道菌群中聚苯乙烯生物降解的探究[J].微生物学通报,2018,45(07):1438-1449.