转录组学主要研究技术及其应用概述
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摘要
转录组学从整体水平上研究基因功能以及基因结构,揭示特定生物学过程以及疾病发生过程中的分子机理。本文介绍转录组学主要研究技术的原理、技术特点及其应用进展。
引文
[1] VELCULESCU VE,ZHANG L,ZHOU W,et al. Characterization of the yeast transcriptome[J]. Cell,1997,88(2):243-251.
[2]周华，张新，刘腾云，等．高通量转录组测序的数据分析与基因发掘[J]．江西科学，2012,30(5):607-611.
[3]李小白，向林，罗洁，等．转录组测序(RNA-Seq)策略及其数据在分子标记开发上的应用[J]．中国细胞生物学学报，2013,35(5):720-726.
[4]郭新红，姜孝成，潘晓玲，等．基因芯片技术与基因表达谱研究[J]．生物学杂志，2001,18(5):1-2.
[5] VELCULESCU VE,ZHANG L,VOGELSTEIN B,et al. Serial analysis of gene expression[J]. Science,1995,270(5235):484-487.
[6] POWELL J. Enhanced concatemer cloninga modification to the SAGE(serial analysis of gene expression)technique[J]. Nucleic Acids Research,1998,26:3445-3446.
[7] BRENNER S, JOHNSON M, BRIDGHAM J, et al. Gene expression analysis by massively parallel signature sequencing(MPSS)on microbead arrays[J]. Nature Biotechnology,2000,18(6):630-634.
[8]陈杰．大规模平行测序技术(MPSS)研究进展[J]．生物化学与生物物理进展，2004,31(8):761-765.
[9] SCHENA M,SHALON D,HELLER R,et al. Parallel human genome analysis:microarray-based expression monitoring of 1000genes[J]. Proceeding of Natural Academy of Science of the United States of Ameirica,1996,93(20):10614-10619.
[10] MA J,DUNEAN D,MORROW DJ,et al. Transcriptome profiling of maize anthers using genetic ablation to analyze pre-meiotic and tapetal cell types[J]. Plant Journal,2007,50:637-648.
[11] LIPSHUTZ D,MORRIS D,CHEE M,et al. Using oligonucleotide probe arrays to access genetic diversity[J]. Biotechniques,1995,19(3):442-447.
[2]周华，张新，刘腾云，等．高通量转录组测序的数据分析与基因发掘[J]．江西科学，2012,30(5):607-611.
[3]李小白，向林，罗洁，等．转录组测序(RNA-Seq)策略及其数据在分子标记开发上的应用[J]．中国细胞生物学学报，2013,35(5):720-726.
[4]郭新红，姜孝成，潘晓玲，等．基因芯片技术与基因表达谱研究[J]．生物学杂志，2001,18(5):1-2.
[5] VELCULESCU VE,ZHANG L,VOGELSTEIN B,et al. Serial analysis of gene expression[J]. Science,1995,270(5235):484-487.
[6] POWELL J. Enhanced concatemer cloninga modification to the SAGE(serial analysis of gene expression)technique[J]. Nucleic Acids Research,1998,26:3445-3446.
[7] BRENNER S, JOHNSON M, BRIDGHAM J, et al. Gene expression analysis by massively parallel signature sequencing(MPSS)on microbead arrays[J]. Nature Biotechnology,2000,18(6):630-634.
[8]陈杰．大规模平行测序技术(MPSS)研究进展[J]．生物化学与生物物理进展，2004,31(8):761-765.
[9] SCHENA M,SHALON D,HELLER R,et al. Parallel human genome analysis:microarray-based expression monitoring of 1000genes[J]. Proceeding of Natural Academy of Science of the United States of Ameirica,1996,93(20):10614-10619.
[10] MA J,DUNEAN D,MORROW DJ,et al. Transcriptome profiling of maize anthers using genetic ablation to analyze pre-meiotic and tapetal cell types[J]. Plant Journal,2007,50:637-648.
[11] LIPSHUTZ D,MORRIS D,CHEE M,et al. Using oligonucleotide probe arrays to access genetic diversity[J]. Biotechniques,1995,19(3):442-447.