硅对砷胁迫下烟草叶绿素荧光特性的影响
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摘要
为揭示硅对砷胁迫下烟草叶绿荧光特性的影响,以漂浮育苗的云烟87为试材,以Na_3AsO_4·12H_2O为砷源,Na_2SiO_3·9H_2O为硅源开展水培试验,试验设置5个处理;对照(CK)、0.25 mmol/L As~(5+)(As_(0.25))、0.35 mmol/L As~(5+)(As_(0.35))、1.0 mmol/L SiO_2+0.25 mmol/L As~(5+)(As_(0.25)+Si)、1.0 mmol/L SiO_2+0.25 mmol/L As~(5+)(As_(0.35)+Si)。结果表明,砷胁迫下烟草φ_(PSⅡ)、F_v/F_m、S_m、φ_(Eo)、ψ_o显著或极显著降低,说明砷胁迫导致PSⅡ反应中心受损,受体侧PQ库容量变小,电子传递受到抑制,从而使电子传递速率下降。硅可使砷胁迫下烟草光反应中心保持较高的活性,使无效能利用所引起的热耗散(DI_o/RC)降低,对光合器官的结构性能参数(PI_(ABS))和单位反应中心的电子传递能力(ET_o/RC)均有增加。
引文
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[2]Zvobgo G,Hu H,Shang S,et al.The effects of phosphate on arsenic uptake and toxicity alleviation in tobacco genotypes with differing arsenic tolerances[J].Environmental Toxicology & Chemistry,2015,34(1):45-52.
[3]常思敏,马新明,赵献章.砷对烤烟生长发育及其产量和品质的影响[J].西北农林科技大学学报(自然科学版),2007,35(8):165-170.
[4]吴玉萍,夏振远,杨虹琦,等.烤烟中砷的检测及砷在烤烟不同部位中的含量[J].中国烟草学报,2008,14(2):35-38.
[5]FDA.Harmful and potentialy harmful constituents in tobaco products and tobaco smoke[EB/OL].[2018-06-10].http://www.fda.gov/TobacoProducts/GuidanceComplianceRegulatoryInformation/ucm297786.htm.
[6]O'Connor R J,Li Q,Stephens W E,et al.Cigarettes sold in China:design,emissions and metals[J].Tob Control,2010,19:47-53.
[7]Gong H J,Chen K M.The regulatory role of silicon on water relations,photosynthetic gas exchange,and carboxylation activities of wheat leaves in field drought conditions[J].Acta Physiologiae Plantarum,2012,34(4):1589-1594.
[8]Balakhnina T I,Bulak P,Matichenkov V V,et al.The influence of Si-rich mineral zeolite on the growth processes and adaptive potential of barley plants under cadmium stress[J].Plant Growth Regulation,2015,75(2):557-565.
[9]Ma J F,Yamaji N.Silicon uptake and accumulation in higher plants[J].Trends in Plant Science,2006,11(8):392-397.
[10]Guo W,Hou Y L,Wang S G,et al.Effect of silicate on the growth and arsenate uptake by rice(Oryza sativa L.) seedlings in solution culture[J].Plant & Soil,2005,272:173-181.
[11]Hu H,Zhang J,Wang H,et al.Effect of silicate supplementation on the alleviation of arsenite toxicity in 93-11 (Oryza sativa L.indica)[J].Environmental Science and Pollution Research,2013,20(12):8579-8589.
[12]Liu W J,Mcgrath S P,Zhao F J.Silicon has opposite effects on the accumulation of inorganic and methylated arsenic species in rice[J].Plant and Soil,2014,376(1):423-431.
[13]Tripathi P,Tripathi R D,Singh R P,et al.Silicon mediates arsenic tolerance in rice(Oryza sativa L.) through lowering of arsenic uptake and improved antioxidant defence system[J].Ecological Engineering,2013,52(2):96-103.
[14]Sanglard L M,Martins S C,Detmann K C,et al.,et al.Silicon nutrition alleviates the negative impacts of arsenic on the photosynthetic apparatus of rice leaves:an analysis of the key limitations of photosynthesis[J].Physiologia Plantarum,2014,152(2):355-366.
[15]石孟春.硅对水稻砷吸收与毒害的影响效应研究[D].南宁:广西大学,2008:13-26.
[16]李懋.硅和秸秆施用对水稻响应旱改水和砷胁迫的影响[D].武汉:华中农业大学,2014:.44-63.
[17]Lou L Q,Shi G L,Wu J H,et al.The influence of phosphorus on arsenic uptake/efflux and as toxicity to wheat roots in comparison with sulfur and silicon[J].Journal of Plant Growth Regulation,2015,34(2):242-250.
[18]王学礼,吕丽兰,黄小青,等.硅钙钾肥对复合污染农田土壤上玉米吸收镉、砷的影响[J].农业研究与应用,2015(3):8-14.
[19]Silva J D,Nascimento C W,Gouveia-Neto A S,et al.Effects of silicon on alleviation arsenic toxicity in maize plants[J].RevistaBrasileira de Ciência do Solo,2015,39(1):289-296.
[20]李慧敏,刘传平,李芳柏,等.叶面喷施铈硅复合溶胶抑制生菜砷积累效应研究[J].生态环境学报,2010,19(5):1108-1113.
[21]Marmiroli M,Pigoni V,Savo-Sardaro M L,et al.The effect of silicon on the uptake and translocation of arsenic in tomato (Solanum lycopersicum L.)[J].Environmental & Experimental Botany,2014,99(1):9-17.
[22]Umar S,Gauba N,Anjum N A,et al.Arsenic toxicity in garden cress (Lepidium sativum Linn.):significance of potassium nutrition.[J].Environmental Science and Pollution Research,2013,20(9):6039-6049.
[23]Srivastava A,Strasser R J,Govindjee.Greening of peas:parallel measurements of 77 K wmission spectra,OJIP chlorophyll a fluorescence transient,period four oscillation of the initial fluorescence level,delayed light emission,and P700[J].Photosynthetica,1999,37(3):365-392.
[24]李鹏民,高辉远,Strasser J.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566.
[25]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学报,1999,16(4):444-448.
[26]Maxwell K,Johnson G N.Chlorophyll fluorescence - a practical guide.[J].Journal of Experimental Botany,2000,51(345):659-668.
[27]Jiang C D,Gao H Y,Zou Q.Changes of donor and acceptor side in photosystem 2 complex induced by iron deficiency in attached soybean and maize leaves[J].Photosynthetica,2003,41(2):267.
[28]李合生.现代植物生理学[M].北京:高等教育出版社,2006:145-149.