蛇床子素壳寡糖纳米胶束中蛇床子素的含量测定
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摘要
目的:建立蛇床子素壳寡糖纳米胶束中蛇床子素的含量测定方法。方法:采用高效液相色谱法测定蛇床子素壳寡糖纳米胶束中蛇床子素的含量:使用AgilentC18柱(4.6×200mm,5μm),流动相为甲醇-0.5%甲酸(75:25,v/v),检测波长322nm,进样量10μl,流速1.0ml/min,柱温25℃。结果:蛇床子素在0.78～50.00μg/ml的浓度范围内峰面积对浓度有良好的线性关系,且方法精密度良好;低、中、高3个浓度蛇床子素的回收率分别为101.7%、98.2%、98.9%,且RSD<2%。3批蛇床子素壳寡糖纳米胶束中蛇床子素的含量分别为154.0μg/ml、167.2μg/ml和175.1μg/ml。结论:本研究中高效液相色谱法测定蛇床子素聚合物胶束中蛇床子素含量的方法具有简便、快速、准确的优点,可用于蛇床子素壳寡糖纳米胶束中蛇床子素的含量测定。
Objective: To establish the method to determine the osthol content in osthol-chitosan nanomicelles. Methods: The content of osthol in osthol-chitosan nanomicelles was determined by high performance liquid chromatography by using Agilent C18 column(24.6×200 mm, 5μm), methanol-0.5% formic acid(75:25, v/v) as mobile phase, detection wavelength 322 nm, flow rate of 1.0 ml/min, and the column temperature was 25℃. Results: The linear range for osthol was in the range of 0.78～50.00μg/ml and the precision of the method was good. The recoveries of low, medium and high concentration osthol were 101.7%, 98.2% and 98.9% respectively, and the RSD was lower 2%. The osthol content in three batches osthol-chitosan nanomicelles were 154.0μg/ml, 167.2μg/ml and 175.1μg/ml. Conclusions: The method established in this study has the advantages of simple, rapid and accurate, and it can be used for determining the osthol content in ostholchitosan nanomicelles.
Objective: To establish the method to determine the osthol content in osthol-chitosan nanomicelles. Methods: The content of osthol in osthol-chitosan nanomicelles was determined by high performance liquid chromatography by using Agilent C18 column(24.6×200 mm, 5μm), methanol-0.5% formic acid(75:25, v/v) as mobile phase, detection wavelength 322 nm, flow rate of 1.0 ml/min, and the column temperature was 25℃. Results: The linear range for osthol was in the range of 0.78～50.00μg/ml and the precision of the method was good. The recoveries of low, medium and high concentration osthol were 101.7%, 98.2% and 98.9% respectively, and the RSD was lower 2%. The osthol content in three batches osthol-chitosan nanomicelles were 154.0μg/ml, 167.2μg/ml and 175.1μg/ml. Conclusions: The method established in this study has the advantages of simple, rapid and accurate, and it can be used for determining the osthol content in ostholchitosan nanomicelles.
引文
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[9]黄传俊,梅勇,杨莉,等.HPLC法同时测定金蝉止痒胶囊中盐酸小檗碱、黄芩苷和蛇床子素的含量[J].中国药房,2018,29(12):1621-1624.
[2]LI L P, WANG X J, ZhANG J Y, et al. Antifungal activity of osthol in vitro and enhancement in vivo through Eudragit S100nanocarriers[J]. Virulence, 2018, 9(1):555-562.
[3]阚默,陈锡俊,兰兴成,等.聚合物胶束脑靶向给药系统的研究[J].长春中医药大学学报,2018,34(2):384-386.
[4]QU X, ZOU Y, HE C, et al. Improved intestinal absorption of paclitaxel by mixed micelles self-assembled from vitamin E succinate-based amphiphilic polymers and their transcellular transport mechanism and intracellular trafficking routes[J]. Drug Deliv, 2018, 25(1):210-225.
[5]徐巍,牟燕,李宏建.聚合物胶束提高难溶性药物生物利用度的研究进展[J].中国药房,2015,26(13):1847-1850.
[6]陈芳,李娟.聚合物胶束载药制备方法研究进展[J].亚太传统医药,2014,10(8):48-49.
[7]宗蕊,沈丽霞.聚合物胶束载药系统的研究进展[J].神经药理学报,2015,5(6):21-29.
[8]汪涛,赵雁平,胡卫东,等.龙藤筋骨片中蛇床子素含量测定研究[J].中国药物应用与监测,2016,1(32):89-91.
[9]黄传俊,梅勇,杨莉,等.HPLC法同时测定金蝉止痒胶囊中盐酸小檗碱、黄芩苷和蛇床子素的含量[J].中国药房,2018,29(12):1621-1624.