玉米GA2ox类基因的发掘及其在苗期干旱胁迫后的表达分析
|
摘要
GA2ox类基因是玉米赤霉素合成途径中的一类负调控基因,其表达产物(GA 2-oxidases)可通过降解赤霉素及其前体物质,调控植物中赤霉素的含量,进而影响植株的诸多表型。研究中发掘到玉米ZmGA2ox类基因13个,进一步分析各基因的基因结构以及在不同组织中的表达状况。通过对各基因干旱胁迫下转录变化的分析发现,只有ZmGA2ox3;1、ZmGA2ox3;2、ZmGA2ox6、ZmGA2ox7;3和ZmGA2ox9共5个ZmGA2ox类基因在干旱胁迫后发生了显著的转录变化。初步鉴定出ZmGA2ox类基因中与玉米耐旱响应相关的候选基因。
The GA2ox like genes are a type of negative regulatory genes in the gibberellin(GA) biosynthesis pathway, and its expression product(GA 2-oxidases) can degrade GAs and its immediate precursor, leading to a lower GA content and reduced growth. In this study, 13 maize ZmGA2ox genes were identified, and the gene structure and their expression in different tissues were also analyzed. Furthermore, the transcription changes were explored in the maize seedlings under the drought stress. The results showed only ZmGA2ox3;1, ZmGA2ox3;2, ZmGA2ox6, ZmGA2ox7;3 and ZmGA2ox9 were significantly upregulated. This study preliminarily identified some ZmGA2ox candidate genes involved in the respond to drought stress.
The GA2ox like genes are a type of negative regulatory genes in the gibberellin(GA) biosynthesis pathway, and its expression product(GA 2-oxidases) can degrade GAs and its immediate precursor, leading to a lower GA content and reduced growth. In this study, 13 maize ZmGA2ox genes were identified, and the gene structure and their expression in different tissues were also analyzed. Furthermore, the transcription changes were explored in the maize seedlings under the drought stress. The results showed only ZmGA2ox3;1, ZmGA2ox3;2, ZmGA2ox6, ZmGA2ox7;3 and ZmGA2ox9 were significantly upregulated. This study preliminarily identified some ZmGA2ox candidate genes involved in the respond to drought stress.
引文
[1]Yamaguchi S.Gibberellin metabolism and its regulation[J].Annual Review of Plant Biology,2008,59(4):225-251.
[2]Pearce S,Huttly A K,Prosser I M,et al.Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family[J].BMC Plant Biology,2015,15(1):1-19.
[3]蒋超，卢天成，李毅丹，等.赤霉素在非生物胁迫中的作用[J].生物技术通报，2016,32(5):11-15.Jiang C,Lu T C,Li Y D,et al.The function of gibberellins signaling in responses to abiotic stresses.biotechnology bulletin[J].Biotechnology Bulletin,2016,32(5):11-15.(in Chinese)
[4]Schomburg F M,Bizzell C M,Lee D J,et al.Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants[J].Plant Cell,2003,15(1):151-163.
[5]Magome H,Yamaguchi S,Hanada A,et al.The DDF1 transcriptional activator upregulates expression of a gibberellin-deactivating gene,GA2ox7,under high-salinity stress in Arabidopsis[J].The Plant Journal,2008,56(4):613-626.
[6]Sakai M,Sakamoto T,Saito T,et al.Expression of novel rice gibberellin 2-oxidase gene is under homeostatic regulation by biologically active gibberellins[J].Journal of Plant Research,2003,116(2):161-164.
[7]Andorf C M,Cannon E K,Portwood J L,et al.Maize GDB update:new tools,data and interface for the maize model organism database[J].Nucleic Acids Research,2016,44(D1):1195-1201.
[8]Chia J M,Song C,Bradbury P J,et al.Maize HapMap2 identifies extant variation from a genome in flux[J].Nature Genetics,2012,44(7):803-807.
[9]Dash S,Van H J,Hong L,et al.PLEXdb:gene resources for plants and plant pathogens[J].Nucleic Acids Research,2012,40(13):1194-1201.
[10]Krugman T,Peleg Z,Quansah L,et al.Alteration in expression of hormone-related genes in wild emmer wheat roots associated with drought adaptation mechanisms[J].Functional&Integrative Genomics,2011,11(4):565-583.
[11]Wang D,Pan Y J,Zhao X Q,et al.Genome-wide temporal-spatial gene expression profiling of drought responsiveness in rice[J].BMCGenomics,2011,12:149.
[12]Sakamoto T,Morinaka Y,Ishiyama K,et al.Genetic manipulation of gibberellin metabolism in transgenic rice[J].Nature Biotechnology,2003,21(8):909-913.
[13]Nelissen H,Rymen B,Jikumaru Y,et al.A local maximum in gibberellin levels regulates maize leaf growth by spatial control of cell division[J].Current Biology,2012,22(13):1183-1187.
[14]Zhou Y,Underhill S J.Breadfruit(Artocarpus altilis)gibberellin 2-oxidase genes in stem elongation and abiotic stress response[J].Plant Physiology and Biochemistry,2016,98:81-88.
[15]Zawaski C,Busov V B.Roles of gibberellin catabolism and signaling in growth and physiological response to drought and short-day photoperiods in Populus trees[J].PLoS One,2014,9(1):e86217.
[16]Shan X H,Li Y D,Jiang Y,et al.Transcriptome profile analysis of maize seedlings in response to high-salinity,drought and cold stresses by deep sequencing[J].Plant Mol Biol Rep,2013,31:1485-1491.
[17]Lo S F,Ho T D,Liu Y L,et al.Ectopic expression of specific GA2oxidase mutants promotes yield and stress tolerance in rice[J].Plant Biotechnol J.,2017,15(7):850-864.
[2]Pearce S,Huttly A K,Prosser I M,et al.Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family[J].BMC Plant Biology,2015,15(1):1-19.
[3]蒋超，卢天成，李毅丹，等.赤霉素在非生物胁迫中的作用[J].生物技术通报，2016,32(5):11-15.Jiang C,Lu T C,Li Y D,et al.The function of gibberellins signaling in responses to abiotic stresses.biotechnology bulletin[J].Biotechnology Bulletin,2016,32(5):11-15.(in Chinese)
[4]Schomburg F M,Bizzell C M,Lee D J,et al.Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants[J].Plant Cell,2003,15(1):151-163.
[5]Magome H,Yamaguchi S,Hanada A,et al.The DDF1 transcriptional activator upregulates expression of a gibberellin-deactivating gene,GA2ox7,under high-salinity stress in Arabidopsis[J].The Plant Journal,2008,56(4):613-626.
[6]Sakai M,Sakamoto T,Saito T,et al.Expression of novel rice gibberellin 2-oxidase gene is under homeostatic regulation by biologically active gibberellins[J].Journal of Plant Research,2003,116(2):161-164.
[7]Andorf C M,Cannon E K,Portwood J L,et al.Maize GDB update:new tools,data and interface for the maize model organism database[J].Nucleic Acids Research,2016,44(D1):1195-1201.
[8]Chia J M,Song C,Bradbury P J,et al.Maize HapMap2 identifies extant variation from a genome in flux[J].Nature Genetics,2012,44(7):803-807.
[9]Dash S,Van H J,Hong L,et al.PLEXdb:gene resources for plants and plant pathogens[J].Nucleic Acids Research,2012,40(13):1194-1201.
[10]Krugman T,Peleg Z,Quansah L,et al.Alteration in expression of hormone-related genes in wild emmer wheat roots associated with drought adaptation mechanisms[J].Functional&Integrative Genomics,2011,11(4):565-583.
[11]Wang D,Pan Y J,Zhao X Q,et al.Genome-wide temporal-spatial gene expression profiling of drought responsiveness in rice[J].BMCGenomics,2011,12:149.
[12]Sakamoto T,Morinaka Y,Ishiyama K,et al.Genetic manipulation of gibberellin metabolism in transgenic rice[J].Nature Biotechnology,2003,21(8):909-913.
[13]Nelissen H,Rymen B,Jikumaru Y,et al.A local maximum in gibberellin levels regulates maize leaf growth by spatial control of cell division[J].Current Biology,2012,22(13):1183-1187.
[14]Zhou Y,Underhill S J.Breadfruit(Artocarpus altilis)gibberellin 2-oxidase genes in stem elongation and abiotic stress response[J].Plant Physiology and Biochemistry,2016,98:81-88.
[15]Zawaski C,Busov V B.Roles of gibberellin catabolism and signaling in growth and physiological response to drought and short-day photoperiods in Populus trees[J].PLoS One,2014,9(1):e86217.
[16]Shan X H,Li Y D,Jiang Y,et al.Transcriptome profile analysis of maize seedlings in response to high-salinity,drought and cold stresses by deep sequencing[J].Plant Mol Biol Rep,2013,31:1485-1491.
[17]Lo S F,Ho T D,Liu Y L,et al.Ectopic expression of specific GA2oxidase mutants promotes yield and stress tolerance in rice[J].Plant Biotechnol J.,2017,15(7):850-864.