摘要
克隆植物对异质生境具有不同的可塑性反应。本研究以克隆植物野牛草(Buchloe dactyloides)相连姊妹分株为材料,利用非损伤微测技术,通过测定正常条件和NaCl胁迫下,野牛草克隆片段姊妹株根尖Ca~(2+)净流速量的变化规律,研究克隆植物根尖Ca~(2+)流对NaCl胁迫的响应,及生理整合对相连克隆构件根尖Ca~(2+)流的调控作用。结果表明,NaCl胁迫促进了野牛草姊株和妹株根尖Ca~(2+)外流,且Ca~(2+)外流主要通过位于质膜上的电压依赖型钙泵完成。野牛草克隆分株间存在NaCl胁迫信号的整合,调控了NaCl胁迫下野牛草分株根尖Ca~(2+)流的变化。Ca~(2+)参与克隆分株对异质营养的感知和应答,调控了克隆分株间的营养生理整合。
Clonal plants exhibit different phenotypic responses to habitat heterogeneity.Ca~(2+) net flux response of root tips to NaCl stress and resultant modification of physiological integration were studied by measuring Ca~(2+) net fluxes of older and younger ramet root tips of Buchloe dactyloides under different NaCl stress treatments using non-invasive micro-test technique.The results showed that NaCl stress increases outflow of Ca~(2+) via voltage-dependent calcium pumps in the plasma membrane.Ca~(2+) net fluxes of root tips were modified by physiological integration of NaCl stress signals between older and younger ramets.Physiological integration was modified by the Ca~(2+) signal of ramets in response to nutrient heterogeneity.
引文
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