家蚕致病性白僵菌的分离鉴定及其在僵蚕人工饲养中的应用
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摘要
为僵蚕人工饲养提供优良菌株,并提高僵蚕的产量和质量,采用形态学观察与分子生物学相结合的方法对从自然感病僵蚕上分离的致病菌株进行鉴定,并研究其人工饲养僵蚕的最适使用浓度。结果表明:该菌株为球孢白僵菌(Beauveria bassiana NB),其最适应用浓度为(1～5)×10~7个/mL,该浓度家蚕僵化死亡率及僵蚕平均单条重量最高,分别为98%和0.9g,僵蚕收益率最大化。
The pathogenesis strain isolated from stiff silkworm naturally infected Beauveria bassiana was identified by using morphological observation and molecular biology and the optimum concentration for artificial feeding of Bombyx batryticatus was studied to provide good strains for artificial feeding of B.batryticatus and improve yield and quality of B.batryticatus.Result:The isolated strain is identified as B.bassiana NB and the optimum application concentration for artificial feeding of B.batryticatus is(1～5)×10~7.The inactivation mortality rate and average weight/stiff silkworm of B.mori larvae inoculated with B.bassiana NB with the optimum application concentration are 98%and 0.9 g(the highest)respectively and the yield rate of B.batryticatus can reach the maximum.
The pathogenesis strain isolated from stiff silkworm naturally infected Beauveria bassiana was identified by using morphological observation and molecular biology and the optimum concentration for artificial feeding of Bombyx batryticatus was studied to provide good strains for artificial feeding of B.batryticatus and improve yield and quality of B.batryticatus.Result:The isolated strain is identified as B.bassiana NB and the optimum application concentration for artificial feeding of B.batryticatus is(1～5)×10~7.The inactivation mortality rate and average weight/stiff silkworm of B.mori larvae inoculated with B.bassiana NB with the optimum application concentration are 98%and 0.9 g(the highest)respectively and the yield rate of B.batryticatus can reach the maximum.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:375.
[2]马继兴.神农本草经辑注[M].北京:人民卫生出版社,1995:327.
[3]王印安.白僵蚕生产初探[J].中药材科技,1993(3):18-19.
[4]张天宝,袁书生.大棚人工生产白僵蚕技术[J].致富天地,2002(11):28.
[5]李冬生,王金华,李世杰,等.僵蚕白僵菌生物学特性的基础研究[J].湖北农业科学,2004(4):101-103.
[6]路国兵,王更先,韩景瑞,等.白僵菌菌株的分离鉴定及生物学特性研究[J].中国蚕业,2007,28(2):13-15.
[7]孙伟屹,张磊,林华峰,等.对家蚕高毒力白僵菌菌株的筛选[J].安徽农业大学学报,2008,35(3):449-451.
[8]宁晓玲.僵蚕价格趋势分析[J].中国现代中药,2011,13(9):53-55.
[9]胡美变,刘玉杰,肖禾,等.僵蚕的人工养殖研究进展及思考[J].中药材,2016,39(4):930-933.
[10]邢东旭,李丽,廖森泰,等.一株感染家蚕的高致病性白僵菌的鉴定及在僵蚕生产中的应用[J].蚕业科学,2014,40(5):879-883.
[11]米红霞,刘吉平.白僵菌生防菌株Bb10和4株家蚕来源白僵菌分离株的生物学特性研究[J].蚕业科学,2010,36(6):962-967.
[12]TAMURA K,NEI M.Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees[J].Molecular Biology and Evolution,1993,10:512-526.
[13]KUMAR S,STECHER G,TAMURA K.MEGA7:Molecular Evolutionary Genetics Analysis version 7.0for bigger datasets[J].Molecular Biology and Evolution,2016,33:1870-1874.
[14]高红,张冉,万永继.白僵菌的分类研究进展[J].蚕业科学,2011,37(4):730-736.
[2]马继兴.神农本草经辑注[M].北京:人民卫生出版社,1995:327.
[3]王印安.白僵蚕生产初探[J].中药材科技,1993(3):18-19.
[4]张天宝,袁书生.大棚人工生产白僵蚕技术[J].致富天地,2002(11):28.
[5]李冬生,王金华,李世杰,等.僵蚕白僵菌生物学特性的基础研究[J].湖北农业科学,2004(4):101-103.
[6]路国兵,王更先,韩景瑞,等.白僵菌菌株的分离鉴定及生物学特性研究[J].中国蚕业,2007,28(2):13-15.
[7]孙伟屹,张磊,林华峰,等.对家蚕高毒力白僵菌菌株的筛选[J].安徽农业大学学报,2008,35(3):449-451.
[8]宁晓玲.僵蚕价格趋势分析[J].中国现代中药,2011,13(9):53-55.
[9]胡美变,刘玉杰,肖禾,等.僵蚕的人工养殖研究进展及思考[J].中药材,2016,39(4):930-933.
[10]邢东旭,李丽,廖森泰,等.一株感染家蚕的高致病性白僵菌的鉴定及在僵蚕生产中的应用[J].蚕业科学,2014,40(5):879-883.
[11]米红霞,刘吉平.白僵菌生防菌株Bb10和4株家蚕来源白僵菌分离株的生物学特性研究[J].蚕业科学,2010,36(6):962-967.
[12]TAMURA K,NEI M.Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees[J].Molecular Biology and Evolution,1993,10:512-526.
[13]KUMAR S,STECHER G,TAMURA K.MEGA7:Molecular Evolutionary Genetics Analysis version 7.0for bigger datasets[J].Molecular Biology and Evolution,2016,33:1870-1874.
[14]高红,张冉,万永继.白僵菌的分类研究进展[J].蚕业科学,2011,37(4):730-736.