蜂胶黄酮作为PPV灭活疫苗佐剂活性的研究

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英文篇名：Adjuvant activity of propolis flavone for PPV vaccine 作者：翟晓虎 ; 徐传芬 ; 张玲玲 ; 贺卫华 ; 马霞 ; 杨俊花 英文作者：ZHAI Xiao-hu;XU Chuan-fen;ZHANG Ling-ling;HE Wei-hua;MA Xia;YANG Jun-hua;Jiangsu Agri-Animal Husbandry Vocational College;Forestry Station in Wennan Town of Xintai;Zhengzhou Institute of City Landscape Architecture Science;Henan University of Animal Husbandry and Economy;Institute for Agri-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences; 关键词：蜂胶黄酮 ; 猪细小病毒疫苗 ; 体液免疫 ; 细胞免疫 英文关键词：Propolis flavone;;Porcine parvovirus vaccine;;Humoral immunity;;Cellular immunity 中文刊名：上海农业学报 英文刊名：Acta Agriculturae Shanghai 机构：江苏农牧科技职业学院;新泰市汶南镇林业站;郑州市城市园林科学研究所;河南牧业经济学院;上海市农业科学院农产品质量标准与检测技术研究所; 出版日期：2019-01-30 出版单位：上海农业学报 年：2019 期：01 基金：江苏省高校品牌专业建设工程资助项目(PPZY2015C230);; 河南牧业经济学院博士科研启动基金(5040229) 语种：中文; 页：76-80 页数：5 CN：31-1405/S ISSN：1000-3924 分类号：S852.4

摘要

为研究蜂胶黄酮不同使用方法对豚鼠体液免疫和细胞免疫的影响,选取99只豚鼠用于试验研究,其中24只用于检验PPV疫苗的安全性,其余75只随机分成5组,分别为蜂胶黄酮佐剂PPV疫苗处理组(PFA)、油佐剂(OA) PPV疫苗+口服蜂胶黄酮处理组(OA-PFApo)、油佐剂(OA) PPV疫苗+肌注蜂胶黄酮处理组(OA-PFAim)、油佐剂PPV疫苗处理组(OA)和生理盐水对照组(CK)。结果表明:与油佐剂PPV疫苗组相比,蜂胶黄酮可以提高豚鼠细胞免疫和早期体液免疫; OA-PFAim组豚鼠体液免疫和细胞免疫均显著增强,而OA-PFApo组豚鼠早期体液免疫效果显著增强。综上,蜂胶黄酮可以直接作为一种PPV疫苗佐剂使用。
        The purpose of this study was to analyze the effect of propolis flavone in different applications on humoral immunity and cellular immunity in immunized guinea pigs. Ninety-nine guinea pigs were selected for this study,and 24 among them were used to determine the safety of PPV vaccine. Other 75 guinea pigs were randomly divided into proplis-flavone-adjuvanted PPV vaccine group( PFA),oil-adjuvanted PPV vaccine oral propolis flavone group( OA-PFApo),oil-adjuvanted PPV vaccine( OA) + propolis flavone by intramuscular injection group( OA-PFAim),oil-adjuvanted PPV vaccine( OA),and physiological salt solution control group. The results showed that,compared with OA group,propolis flavone adjuvant could enhance cellular immunity and early humoral immunity in immunized guinea pigs. In OA-PFAim group,cellular immunity and humoral immunity in guinea pigs were both significantly increased. In OA-PFApo group,early humoral immune was significantly enhanced. It's concluded that propolis flavone can be used as adjuvant to prepare propolis flavone PPV vaccine.

引文

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