鼠李糖脂发酵液驱油性能研究
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摘要
针对鼠李糖脂在油田开发中存在的驱油体系单一和机理认识不清的问题,开展了相关的驱油机理和驱油性能研究。室内发酵出以单、双糖环鼠李糖脂为主的两种生物表面活性剂发酵液,评价了其界面性能、润湿性、洗油效率和驱油效率。研究发现,鼠李糖脂发酵液耐温抗盐性强,1%发酵液与原油间界面张力为0.2～0.5 mN/m。润湿性和洗油效率评价显示,双糖环鼠李糖脂润湿性改善更突出,洗油效率比单糖环鼠李糖脂提高15.0%,达到90.1%。分析认为,鼠李糖脂能将原油与基底间黏附功由30.3 mN/m最低降至0.059 8 mN/m(双糖环鼠李糖脂),是发酵液提高洗油效率的关键因素。驱油效率评价结果表明,双糖环鼠李糖脂发酵液在水驱效率42.16%基础上提高驱油效率12.3%,展现出较强的现场应用潜力。
In view of the unvaried nature of current oil displacement systems and uncertainty about the action of rhamnolipids in oil displacement and oil field development,the mechanisms and performance of rhamnolipids in oil displacement were investigated in the present study.Fermentation broths consisting primarily of two types of biosurfactants,viz.mono-rhamnolipids and di-rhamnolipids,were obtained via indoor fermentation,and the interfacial performance,wettability,oil washing efficiency,and oil displacement efficiency of the fermentation broths were assessed.The rhamnolipid fermentation broths were highly resistant to heat and salt,and the interfacial tensions between the 1 % fermentation broths and crude oil were 0.2～0.5 mN/m.Assessments of the wettability and oil washing efficiency of the fermentation broths showed that di-rhamnolipids resulted in a greater improvement in wettability and achieved an oil washing efficiency of 90.1%,an increase of 15 % compared to that of the mono-rhamnolipids.It was found that rhamnolipids could reduce the work of adhesion between crude oil and the substrate from 30.2 mN/m to a minimum of 0.059 8 mN/m(achieved with di-rhamnolipids),and that this was a key factor in the enhancement of oil washing efficiency by the fermentation broths.Assessment of oil displacement efficiency revealed that the di-rhamnolipid fermentation broth increased the oil displacement efficiency by 12.3 % based on a water-oil displacement efficiency of 42.16%,thereby exhibiting great potential for field applications.
In view of the unvaried nature of current oil displacement systems and uncertainty about the action of rhamnolipids in oil displacement and oil field development,the mechanisms and performance of rhamnolipids in oil displacement were investigated in the present study.Fermentation broths consisting primarily of two types of biosurfactants,viz.mono-rhamnolipids and di-rhamnolipids,were obtained via indoor fermentation,and the interfacial performance,wettability,oil washing efficiency,and oil displacement efficiency of the fermentation broths were assessed.The rhamnolipid fermentation broths were highly resistant to heat and salt,and the interfacial tensions between the 1 % fermentation broths and crude oil were 0.2～0.5 mN/m.Assessments of the wettability and oil washing efficiency of the fermentation broths showed that di-rhamnolipids resulted in a greater improvement in wettability and achieved an oil washing efficiency of 90.1%,an increase of 15 % compared to that of the mono-rhamnolipids.It was found that rhamnolipids could reduce the work of adhesion between crude oil and the substrate from 30.2 mN/m to a minimum of 0.059 8 mN/m(achieved with di-rhamnolipids),and that this was a key factor in the enhancement of oil washing efficiency by the fermentation broths.Assessment of oil displacement efficiency revealed that the di-rhamnolipid fermentation broth increased the oil displacement efficiency by 12.3 % based on a water-oil displacement efficiency of 42.16%,thereby exhibiting great potential for field applications.
引文
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[28] STROM G,FREDRIKSSON M,STENIUS P.Contact angles,work of adhesion,and interfacial tensions at a dissolving hydrocarbon surface[J].Journal of colloid and interface science,1987,119(2):352-361.doi:10.-1016/0021-9797(87)90280-3
[29]谢坤,卢祥国,陈欣,等高温低渗油藏中表面活性剂溶液渗吸效果影响因素研究[J].西安石油大学报(自然科学版),2015,30(5):80-84.doi:10.3969/j.-issn.1673-064X.2015.05.014XTE Kun,LU Xiangguo,CHEN Xin,et al.Study on influence factors of imbibition effect of surfactant in hightemperature and low permeability reservoir[J].Journal of Xi'an Shiyou University(Natural Science Edition),2015,30(5):80-84.doi:10.3969/j.issn.1673-064X.2015.05.014
[2]计秉玉.国内外油田提高采收率技术进展与展望[J].石油与天然气地质,2012,33(1):111-117.JI Bingyu.Progress and prospects of enhanced oil recovery technologies at home and abroad[J].Oil&Gas Geology2012,33(1):111-117.
[3]朱友益,侯庆锋,简国庆,等.化学复合驱技术研究与应用现状及发展趋势[J].石油勘探与开发,2013,40(1):90-96.ZHU Youyi,HOU Qingfeng,JIAN Guoqing,et al.Current development and application of chemical combination flooding technique[J].Petroleum Exploration and Development,2013,40(1):90-96.
[4]NEDJHIOUI M,MOULAI-MOSTEFA N,MORSLI A,et al.Combined effects of polymer/surfactant/oil/alkali on physical chemical properties[J].Desalination,2005,185(1-3):543-550.doi:10.1016/j.desal.2005.05.013
[5]HIRASAKI G J,MILLER C A,PUERTO M.Recent advances in surfactant EOR[C].SPE 115386-MS,2008.doi:10.2118/115386-MS
[6]郑可,徐怀民,陈建文.改性注入剂对改善特低渗储层渗流能力的实验研究及应用[J].天然气地球科学,2013,24(4):832-841.ZHENG Ke,XU Huaimin,CHEN Jianwen.The influence of modified injectant on improvement of percolation ability in super-low permeability reservoirs:Experimental study and application[J].Natural Gas Geoscience,2013.24(4):832-841.
[7] BELLO X V,DEVESAREY R,CRUZ J M,et al.Study of the synergistic effects of salinity,pH,and temperature on the surface-active properties of biosurfactants produced by lactobacillus pentosus[J].Journal of Agricultural and Food Chemistry,2012,60(5):1258-1265.doi:10.-1021/jf205095d
[8]PUERTO M,MILLER C A,HIRASAKI G J,et al.Surfactant systems for EOR in high-temperature,highsalinity environments[C].SPE 129675-MS,2010.doi:10.2118/129675-MS
[9]JOSHI S,BHARUCHA C,JHA S,et al.Biosurfactant production using molasses and whey under thermophilic conditions[J].Bioresour Technol,2008,99(1):195-199.
[10]MAKKAR R S,CAMEOTRA S S.An update on the use of unconventional substrates for biosurfactant production and their new applications[J].Applied Microbiology and Biotechnology,2002,58(4):428-434.doi:10.-1007/s00253-001-0924-1
[11] SOUAYEH M,ALWAHAIBI Y M,ALBAHRY S,et al.Optimization of a low-concentration bacillus subtilis strain biosurfactant toward microbial enhanced oil recovery[J].Energy&Fuels,2014,66(6):1930-1933.doi:10.1021/ef500954u
[12]NGUYEN T T,YOUSSEF N H,MCINERNEY M J,et al.Rhamnolipid biosurfactant mixtures for environmental remediation[J].Water Research,2008,42(6-7):1735-1743.doi:10.1016/j.watres.2007.10.038
[13]AL-SULAIMANIH,AL-WAHAIBI Y,AL-BAHRY S,et al.Residual-oil recovery through injection of biosurfactant,chemical surfactant,and mixtures of both under reservoir temperatures:induced-wettability and interfacialtension effects[J].SPE Reservoir Evaluation&Engineering,2012,15(2):210-217.doi:10.2118/158022-PA
[14]AL-WAHAIBI Y,JOSHI S,AL-BAHRY S,et al.Biosurfactant production by Bacillus subtilis B30 and its application in enhancing oil recovery[J].Colloids and Surfaces B:Biointerfaces,2014,114(8):324-333.doi:10 1016/j.-colsurfb.2013.09.022
[15]SIM L,WARD O,LI Z.Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1[J].Journal of Industrial Microbiology and Biotechnology,1997,19(4):232-238.doi:10.1038/sj.-jim.2900450
[16]SATPUTE S K,BANPURKAR A G,DHAKEPHALKAR P K,et al.Methods for investigating biosurfactants and bioemulsifiers:A review[J].Critical Reviews in Biotechnology,2010,30(2):127-144.doi:10.3109/07388550903427280
[17]MATA-SANDOVAL J C,Karns J,TORRENTS A.Effect of nutritional and environmental conditions on the production and composition of rhamnolipids by P.aeruginosa UG2[J].Microbiological Research,2001,155(4):249-256.doi:10.1016/S0944-5013(01)80001-X
[18]MULLer M M,HORMANN B,SYLDATK C,et al.Pseudomonas aeruginosa PAO1 as a model for rhamnolipid production in bioreactor systems[J].Applied Microbiology and Biotechnology,2010,87(1):167-174.doi:10.-1007/s00253-010-2513-7
[19]ROBERT M,MERCADE M,BOSCH M,et al.Effect of the carbon source on biosurfactant production by Pseudomonas aeruginosa 44T1[J].Biotechnology Letters,1989,11(12):871-874.doi:10.1007/BF01026843
[20]ONWOSIC O,ODIBO F J C.Effects of carbon and nitrogen sources on rhamnolipid biosurfactant production by Pseudomonas nitroreducens isolated from soil[J]. World Journal of Microbiology and Biotechnology,2012,28(3):937-942.doi:10.1007/s11274-011-0891-3
[21]GUDINA E J,RODRIGUES A I,ALVES E,et al.Bioconversion of agro-industrial by-products in rhamnolipids toward applications in enhanced oil recovery and bioremediation[J].Bioresource Technology,2015,177:87-93.doi:10.1016/j.biortech.2014.11.069
[22]RAHMAN P K,PASIRAYI G,AUGER V,et al.Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor[J].Biotechnology and Applied Biochemistry,2010,55(1):45-52.doi:10.1042/BA20090277|
[23] EL ZEFTAWY M M,MULLIGAN C N.Use of rhamnolipid to remove heavy metals from wastewater by micellarenhanced ultrafiltration(MEUF)[J].Separation and Purification Technology,2011,77(1):120-127.doi:10.-1016/j.seppur.2010.11.030
[24] VARJANI S J,UPASANI V N.Core flood study for en-hanced oil recovery through ex-situ bioaugmentation with thermo-and halo-tolerant rhamnolipid produced by Pseudomonas aeruginosa NCIM 5514[J].Bioresource Technology,2016,220:175-182.doi:10.1016/j.biortech.2016.-08.060
[25]YOUYI Z,QINGFENG H,GUOQING J,et al.Current development and application of chemical combination flooding technique[J].Petroleum Exploration and Development,2013,40(1):96-103.doi:10.1016/S1876-3804(13)60009-9
[26]王成文,郑飞,孙晗森,等.改善煤岩润湿性的强化煤层固井胶结技术[J].天然气工业,2017,37(7):83-88doi:10.3787/j.issn.1000-0976.2017.07.012WANG Chengwen,ZHENG Fei,SUN Hansen,et al.Bonding-strengthening technology in coalbed cementing through wettability improvement[J].Natural Gas Industry,2017,37(7):83-88.doi:10.3787/j.issn.1000-0976.2017.-07.012
[27]侯宝峰.表面活性剂改变岩石表面润湿性及其提高采收率研究[D].青岛:中国石油大学(华东),2016.HOU Baofeng.Study on wettability alteration of rock surface and its EOR induced by surfactants[D].Qingdao:China University of Petroleum(East China),2016.
[28] STROM G,FREDRIKSSON M,STENIUS P.Contact angles,work of adhesion,and interfacial tensions at a dissolving hydrocarbon surface[J].Journal of colloid and interface science,1987,119(2):352-361.doi:10.-1016/0021-9797(87)90280-3
[29]谢坤,卢祥国,陈欣,等高温低渗油藏中表面活性剂溶液渗吸效果影响因素研究[J].西安石油大学报(自然科学版),2015,30(5):80-84.doi:10.3969/j.-issn.1673-064X.2015.05.014XTE Kun,LU Xiangguo,CHEN Xin,et al.Study on influence factors of imbibition effect of surfactant in hightemperature and low permeability reservoir[J].Journal of Xi'an Shiyou University(Natural Science Edition),2015,30(5):80-84.doi:10.3969/j.issn.1673-064X.2015.05.014