GA_3和ABA对马铃薯薯形的影响
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摘要
采用高效液相色谱串联质谱法测定马铃薯2个不同薯形品种会-2和PB06成熟块茎中植物激素赤霉素(GA_3)及脱落酸(ABA)的含量,发现椭圆薯形品种会-2成熟块茎中GA_3含量高、ABA含量低,相反圆薯形品种PB06块茎中GA_3含量低、ABA含量高。由此推测,植物激素ABA和GA_3可能参与调控马铃薯块茎形状的发育。外源添加植物生长调节剂GA_3及ABA同样影响试管薯的形状。添加0.01μmol/L GA_3,试管薯纵茎与横径的比值增加到1.50;添加5μmol/L ABA,块茎纵径与横茎的比值轻微下降,与对照相比没有显著差异。但是同时添加0.01μmol/L GA_3和5μmol/L ABA,试管薯纵茎与横径的比值相对单独添加0.01μmol/L GA_3显著降低到1.37。因此,ABA和GA_3在调控试管薯的薯形中起到拮抗效应,该作用可能是通过影响薯形相关基因的表达或关闭而实现的。
引文
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[2]Uitdewilligen J.Discovery and genotyping of existing and induced DNA sequence variation in potato[D].Wageningen:Wageningen University,2012.
[3]van Eck H J.Genetics of morphological and tuber traits[J].Potato Biology and Biotechnology,2007,91-115.
[4]Salaman R N.The inheritance of colour and other characters in the potato[J].Journal of Genetics,1911,5(1):192-193.
[5]Jong H D,Bums V J.Inheritance of tuber shape in cultivated diploid potatoes[J].American Potato Journal,1993,70(3):267-284.
[6]Masson M F.Mapping,combining abilities,heritabilities and heterosis with 4X×2X crosses in potato[D].Madison:Wisconsin University,1985.
[7]van Eck H J,Jacobs J M,Stam P,et al.Multiple alleles for tuber shape in diploid potato detected by qualitative and quantitative genetic analysis using RFLPs[J].Genetics,1994,137(1):303-309.
[8]Sliwka J,Wasilewicz-Flis I,Jakuczun H,et al.Tagging quantitative trait loci for dormancy,tuber shape,regularity of tuber shape,eye depth and flesh color in diploid potato originated from six Solanum species[J].Plant Breed,2008,127(1):49-55.
[9]Prashar A,Hornyik C,Young V,et al.Construction of a dense SNPmap of a highly heterozygous diploid potato population and QTLanalysis of tuber shape and eye depth[J].Theoretical and Applied Genetics,2014,127(10):2159-2171.
[10]D'Hoop B B,Keizer P L C,Paulo M J,et al.Identification of agronomically important QTL in tetraploid potato cultivars using a marker-trait association analysis[J].Theoretical and Applied Genetics,2014,127(3):731-748.
[11]朱文文,徐建飞,李广存,等.马铃薯块茎形状基因CAPS标记的开发与验证[J].作物学报,2015,41(10):1529-1536.
[12]沈云龙.马铃薯薯形相关基因序列分析及光敏色素影响块茎形成初步研究[D].武汉:华中农业大学,2013.
[13]陈娜.马铃薯薯形基因位点标记开发与物理图谱构建[D].北京:中国农业科学院,2017.
[14]Dutt S,Manjul A S,Raigond,et al.Key players associated with tuberization in potato:potential candidates for genetic engineering[J].Critical Reviews in Biotechnology,2017,37(7):942-957.
[15]Liu X,Yang Y L,Lin W H,et al.Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry[J].Chinese Science Bulletin,2010,55(21):2231-2235.
[16]Zhou L J,Xiao L T,Xue H W.Dynamic cytology and transcriptional regulation of rice lamina joint development[J].Plant Physiology,2017,174(3):1728-1746.
[17]Shu K,Zhang H W,Wang S F,et al.ABI4 regulates primary seed dormancy by regulating the biogenesis of abscisic acid and gibberellins in Arabidopsis[J].PLo S Genetics,2013,9(6):e1003577.
[18]Shu K,Zhou W G,Yang W Y.APETALA 2-domain-containing transcription factors:focusing on abscisic acid and gibberellins antagonism[J].New Phytologist,217(3):977-983.
[19]Xu X,Lammeren A A M V,Vermeer E,et al.The role of gibberellin,abscisic acid,and sucrose in the regulation of potato tuber formation in vitro[J].Plant Physiology,1998,117(2):575-584.
[20]Noelia M G M,Stritzler M,Capiati D A.Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation[J].Planta,2014,239(3):615-631.
[21]胡月清,詹爽,库文珍,等.ABA和GA3对马铃薯试管薯形成及淀粉积累的协同调控[J].分子植物育种,2017,15(10):4210-4214.