热应激对黄羽肉鸡心脏UQCRB基因表达的影响研究
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摘要
[目的]研究热应激对黄羽肉鸡生长性能的影响。[方法]对新广黄公鸡和怀乡公鸡进行热应激处理,应用荧光定量PCR法检测UQCRB基因在新广黄公鸡和怀乡公鸡心脏组织中的表达水平,分析其对黄羽肉鸡体重、心脏相对重和心脏UQCRB基因相对表达量的影响。[结果]新广黄公鸡和怀乡公鸡21、28 d体重以及28 d心脏绝对重显著高于怀乡公鸡(P<0.05),2种公鸡心脏相对重差异不显著。28 d试验组新广黄公鸡的体重显著低于对照组(P<0.05)。新广黄公鸡21 d心脏UQCRB基因的相对表达量显著高于怀乡公鸡(P<0.05)。热应激显著降低了新广黄公鸡28 d心脏UQCRB基因的相对表达量(P<0.05)。[结论]心脏UQCRB基因表达与黄羽肉鸡生长速度相关,在肉鸡其他组织器官的表达有待进一步研究。
[Objective]To study the effects of heat stress on the growth performance of yellow-feather broilers.[Method] The heat stress treatment were conducted on Xinguanghuang cock and Huaixiang cock.Fluorescence quantitative PCR was used to analyze the expression level of UQCRB gene in the heart tissues of Xinguanghuang cock and Huaixiang cock.The effects of heat stress treatment on the body weight,the relative weight of heart and the relative expression of UQCRB gene in the heart of yellow-feather broilers were analyzed.[Result]The body weight on the 21~(st),28~(th) day,and heart weight on the 28~(th) day of Xinguanghuang cock were significantly higher than those of Huaixiang cock(P<0.05),but the relative weight of heart had no significant difference between Xinguanghuang cock and Huaixiang cock.The body weight of Xinguanghuang cock on the 28~(th) day in test group was significantly lower than that in control group(P<0.05). The relative expression of UQCRB gene in the heart of Xinguanghuang cock on the 21~(st) day was significantly higher than that in Huaixiang cock(P<0.05). Heat stress significantly reduced the relative expression of UQCRB gene in the heart of Xinguanghuang cock(P<0.05).[Conclusion]The expression of UQCRB gene in the heart was related to the growth rate of yellow-feather broilers.The expression of UQCRB gene in other tissues of yellow-feather broilers needs further study.
[Objective]To study the effects of heat stress on the growth performance of yellow-feather broilers.[Method] The heat stress treatment were conducted on Xinguanghuang cock and Huaixiang cock.Fluorescence quantitative PCR was used to analyze the expression level of UQCRB gene in the heart tissues of Xinguanghuang cock and Huaixiang cock.The effects of heat stress treatment on the body weight,the relative weight of heart and the relative expression of UQCRB gene in the heart of yellow-feather broilers were analyzed.[Result]The body weight on the 21~(st),28~(th) day,and heart weight on the 28~(th) day of Xinguanghuang cock were significantly higher than those of Huaixiang cock(P<0.05),but the relative weight of heart had no significant difference between Xinguanghuang cock and Huaixiang cock.The body weight of Xinguanghuang cock on the 28~(th) day in test group was significantly lower than that in control group(P<0.05). The relative expression of UQCRB gene in the heart of Xinguanghuang cock on the 21~(st) day was significantly higher than that in Huaixiang cock(P<0.05). Heat stress significantly reduced the relative expression of UQCRB gene in the heart of Xinguanghuang cock(P<0.05).[Conclusion]The expression of UQCRB gene in the heart was related to the growth rate of yellow-feather broilers.The expression of UQCRB gene in other tissues of yellow-feather broilers needs further study.
引文
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[13] VAN GOOR A,BOLEK K J,ASHWELL C M,et al.Identification of quantitative trait loci for body temperature,body weight,breast yield,and digestibility in an advanced intercross line of chickens under heat stress[J].Genetics selection evolution,2015,47(1):1-13.
[14] ZHANG J B,SCHMIDT C J,LAMONT S J.Transcriptome analysis reveals potential mechanisms underlying differential heart development in fast- and slow-growing broilers under heat stress[J].BMC Genomics,2017,18(1):1-15.
[15] VAN DE VEN L J F,VAN WAGENBERG A V,DECUYPERE E,et al.Perinatal broiler physiology between hatching and chick collection in 2 hatching systems[J].Poultry science,2013,92(4):1050-1061.
[2] SOHAIL M U,HUME M E,BYRD J A,et al.Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress[J].Poultry science,2012,91(9):2235-2240.
[3] LIMONGELLI G,TOME-ESTEBAN M,DEJTHEVAPORN C,et al.Prevalence and natural history of heart disease in adults with primary mitochondrial respiratory chain disease[J].Eur J Heart Fail,2010,12(2):114-121.
[4] FINSTERER J,KOTHARI S.Cardiac manifestations of primary mitochondrial disorders[J].Int J Cardiol,2014,177(3):754-763.
[5] OLKOWSKI A A.Pathophysiology of heart failure in broiler chickens:Structural,biochemical,and molecular characteristics[J].Poultry science,2007,86(5):999-1005.
[6] BAGHBANZADEH A,DECUYPERE E.Ascites syndrome in broilers:Physiological and nutritional perspectives[J].Avian pathology,2008,37(2):117-126.
[7] JUNG H J,SHIM J S,LEE J Y,et al.Terpestacin inhibits tumor angiogenesis by targeting UQCRB of mitochondrial complex III and suppressing hypoxia-induced reactive oxygen species production and cellular oxygen sensing[J].Journal of biological chemistry,2010,285(15):11584-11595.
[8] JUNG H J,KIM K H,KIM N D,et al.Identification of a novel small molecule targeting UQCRB of mitochondrial complex III and its anti-angiogenic activity[J].Bioorg Med Chem Lett,2011,21(3):1052-1056.
[9] CHANG J,JUNG H J,PARK H J,et al.Cell-permeable mitochondrial ubiquinol-cytochrome c reductase binding protein induces angiogenesis in vitro and in vivo[J].Cancer Lett,2015,366(1):52-60.
[10] 钟光,邵丹,施寿荣,等.循环热应激对黄羽肉鸡呼吸机能、生长性能和肝脏抗氧化功能的影响[J].动物营养学报,2018,30(3):947-952.
[11] 郭天宇,王江水,李凯旋,等.环境温度对黄羽肉鸡后期生产性能、消化功能和热应激相关指标的影响[J].中国畜牧杂志,2018,54(8):107-111.
[12] DEEB N,LAMONT S J.Genetic architecture of growth and body composition in unique chicken populations[J].Journal of heredity,2002,93(2):107-118.
[13] VAN GOOR A,BOLEK K J,ASHWELL C M,et al.Identification of quantitative trait loci for body temperature,body weight,breast yield,and digestibility in an advanced intercross line of chickens under heat stress[J].Genetics selection evolution,2015,47(1):1-13.
[14] ZHANG J B,SCHMIDT C J,LAMONT S J.Transcriptome analysis reveals potential mechanisms underlying differential heart development in fast- and slow-growing broilers under heat stress[J].BMC Genomics,2017,18(1):1-15.
[15] VAN DE VEN L J F,VAN WAGENBERG A V,DECUYPERE E,et al.Perinatal broiler physiology between hatching and chick collection in 2 hatching systems[J].Poultry science,2013,92(4):1050-1061.