北极苔原土壤中可培养细菌的分离及其抗菌活性测定
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摘要
【目的】北极地区具有高纬度、低温、高辐射等独特的环境条件。北冰洋及周围大面积的陆地区域鲜有人类踪迹,其中微生物数量不可低估。本研究旨在了解北极土壤中的可培养微生物的多样性及其抗菌活性。【方法】对来源于北极黄河站附近的7份不同植物根下苔原土壤进行直接涂布和富集培养后涂布。【结果】共获得细菌菌株721株,对其中608株进行细菌16S rRNA基因序列测定,归属于86个属,229个种,主要分布于变形菌门(Proteobacteria,54.3%)、放线菌门(Actinobacteria,21.2%)、拟杆菌门(Bacteroidetes,12.8%)、厚壁菌门(Firmicutes,10.0%)和奇异球菌门(Deinococcus-Thermus,1.6%)。其中从16S rRNA基因序列同源性推测有22株细菌菌株为潜在新种/属。从分离菌株中筛选出16株可抑制金黄色葡萄球菌(Staphylococcusaureus)或鲍氏不动杆菌(Acinetobacterbaumannii)生长的拮抗菌。【结论】获得了北极土壤地区特有的微生物菌株资源,为进一步筛选拮抗菌的活性物质提供了菌株基础。
[Objective] The Arctic region has unique environmental characteristics such as high latitude, low temperature and high radiation. There are few human traces in the Arctic Ocean and the surrounding land area, but the number of microorganisms hidden in Arctic region is underestimated. We explored the culturable bacterial diversity and antibacterial activity in Arctic tundra soil. [Methods] We isolated bacteria from 7 soil samples collected from different plant roots near the Yellow River Station in Ny-?lesund(Svalbard) Arctic. [Results] A total of 721 strains were isolated from samples, 608 strains of which belong to 86 genera and 229 species after 16 S rRNA gene sequencing. These 16 S rRNA gene sequences mainly grouped into the following 5 phyla: Proteobacteria(54.3%), Actinobacteria(21.2%), Bacteroidetes(12.8%), Firmicutes(10.0%) and Deinococcus-Thermus(1.6%). It was suggested 22 strains could be potential novel species for their low sequence similarity. Furthermore, 16 antibacterial strains which could inhibit the growth of Staphylococcus aureus or Acinetobacter baumannii were screened. [Conclusion] The research obtained special bacteria resource of arctic soil, and solidified the foundation of studying bioactive substances.
[Objective] The Arctic region has unique environmental characteristics such as high latitude, low temperature and high radiation. There are few human traces in the Arctic Ocean and the surrounding land area, but the number of microorganisms hidden in Arctic region is underestimated. We explored the culturable bacterial diversity and antibacterial activity in Arctic tundra soil. [Methods] We isolated bacteria from 7 soil samples collected from different plant roots near the Yellow River Station in Ny-?lesund(Svalbard) Arctic. [Results] A total of 721 strains were isolated from samples, 608 strains of which belong to 86 genera and 229 species after 16 S rRNA gene sequencing. These 16 S rRNA gene sequences mainly grouped into the following 5 phyla: Proteobacteria(54.3%), Actinobacteria(21.2%), Bacteroidetes(12.8%), Firmicutes(10.0%) and Deinococcus-Thermus(1.6%). It was suggested 22 strains could be potential novel species for their low sequence similarity. Furthermore, 16 antibacterial strains which could inhibit the growth of Staphylococcus aureus or Acinetobacter baumannii were screened. [Conclusion] The research obtained special bacteria resource of arctic soil, and solidified the foundation of studying bioactive substances.
引文
[1]Cheng ZB,Shi XF,Chen ZH,Wu YH,Li XY,Ju XH,Shi FD.2007 summer scientific expedition to and general information on Chinese arctic yellow river station.Advances in Marine Science,2008,26(1):112-118.(in Chinese)程振波,石学法,陈志华,吴永华,李小艳,鞠晓华,石丰登.2007中国北极黄河站夏季科学考察及黄河站概况.海洋科学进展,2008,26(1):112-118.
[2]Deng HB,Lu LH,Bian LG.Surface radiation characteristics at Ny-?lesund over the arctic tundra area.Chinese Journal of Polar Research,2006,18(4):254-264.(in Chinese)邓海滨,陆龙骅,卞林根.北极苔原新奥尔松地区的地表辐射特征.极地研究,2006,18(4):254-264.
[3]Yao YF,Cao SN,Peng F,Li CS.Characteristics of species composition and community structure on the Austre Lovénbreen glacier foreland,Ny-?lesund,Svalbard.Chinese Journal of Polar Research,2014,26(3):362-368.(in Chinese)姚轶锋,曹叔楠,彭方,李承森.北极新奥尔松Austre Lovénbreen冰川退缩迹地不同演替阶段的植物组成与植被群落特征分析.极地研究,2014,26(3):362-368.
[4]Kim M,Jung JY,Laffly D,Kwon HY,Lee YK.Shifts in bacterial community structure during succession in a glacier foreland of the High Arctic.FEMS Microbiology Ecology,2017,93(1):fiw213.
[5]Neufeld JD,Mohn WW.Unexpectedly high bacterial diversity in arctic tundra relative to boreal forest soils,revealed by serial analysis of ribosomal sequence tags.Applied and Environmental Microbiology,2005,71(10):5710-5718.
[6]Peleg AY,Seifert H,Paterson DL.Acinetobacter baumannii:emergence of a successful pathogen.Clinical Microbiology Reviews,2008,21(3):538-582.
[7]Lee CR,Lee JH,Park M,Park KS,Bae IK,Kim YB,Cha CJ,Jeong BC,Lee SH.Biology of Acinetobacter baumannii:Pathogenesis,antibiotic resistance mechanisms,and prospective treatment options.Frontiers in cellular and infection microbiology,2017,7(55):1:35.
[8]Zhang XL,Li XH,Zhang F,Chen SC.Drug resistance analysis of staphylococcus aureus strains in Beijing Luhe Hospital in 2016.Medical Recapitulate,2018,24(2):409-412.(in Chinese)张晓兰,李晓辉,张芳,陈世财.医院2016年金黄色葡萄球菌耐药性分析.医学综述,2018,24(2):409-412.
[9]Kim MK,Park H,Oh TJ.Antibacterial and antioxidant capacity of polar microorganisms isolated from Arctic lichen Ochrolechia sp.Polish Journal of Microbiology,2014,63(3):317-322.
[10]Kim MK,Park H,Oh TJ.Antimicrobial properties of the bacterial associates of the Arctic lichen Stereocaulon sp.African Journal of Microbiology Research,2013,7:3651-3657.
[11]Liu QQ,Wang Y,Li J,Du ZJ,Chen GJ.Saccharicrinis carchari sp.nov.,isolated from a shark,and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans.International Journal of Systematic and Evolutionary Microbiology,2014,64(7):2204-2209.
[12]Nissinen RM,M?nnist?MK,van Elsas JD.Endophytic bacterial communities in three arctic plants from low arctic fell tundra are cold-adapted and host-plant specific.FEMSMicrobiology Ecology,2012,82(2):510-522.
[13]K?mpfer P,McInroy JA,Glaeser SP.Chryseobacterium zeae sp.nov.,Chryseobacterium arachidis sp.nov.,and Chryseobacterium geocarposphaerae sp.nov.isolated from the rhizosphere environment.Antonie van Leeuwenhoek,2014,105(3):491-500.
[14]K?mpfer P,McInroy JA,Glaeser SP.Chryseobacterium rhizoplanae sp.nov.,isolated from the rhizoplane environment.Antonie van Leeuwenhoek,2015,107(2):533-538.
[15]Du J,Ngo HTT,Won K,Kim KY,Jin FX,Yi TH.Chryseobacterium solani sp.nov.,isolated from field-grown eggplant rhizosphere soil.International Journal of Systematic and Evolutionary Microbiology,2015,65(8):2372-2377.
[16]Pot B,Willems A,Gillis M,de Ley J.Intra-and Intergeneric relationships of the genus Aquaspirillum:Prolinoborus,a New Genus for Aquaspirillum fasciculus,with the species Prolinoborus fasciculus comb.nov.International Journal of Systematic and Evolutionary Microbiology,1992,42(1):44-57.
[17]LabbéN,Parent S,Villemur R.Nitratireductor aquibiodomus gen.nov.,sp.nov.,a novelα-proteobacterium from the marine denitrification system of the Montreal Biodome(Canada).International Journal of Systematic and Evolutionary Microbiology,2004,54(1):269-273.
[18]Bajerski F,Ganzert L,Mangelsdorf K,Lipski A,Wagner D.Cryobacterium arcticum sp.nov.,a psychrotolerant bacterium from an arctic soil.International Journal of Systematic and Evolutionary Microbiology,2011,61(8):1849-1853.
[19]Reddy GSN,Pradhan S,Manorama R,Shivaji S.Cryobacterium roopkundense sp.nov.,a psychrophilic bacterium isolated from glacial soil.International Journal of Systematic and Evolutionary Microbiology,2010,60(4):866-870.
[20]Liu Q,Xin YH,Chen XL,Liu HC,Zhou YG,Chen WX.Cryobacterium aureum sp.nov.,a psychrophilic bacterium isolated from glacier ice collected from the ice tongue surface.International Journal of Systematic and Evolutionary Microbiology,2018,68(4):1173-1176.
[21]Liu Q,Liu HC,Wen Y,Zhou YG,Xin YH.Cryobacterium flavum sp.nov.and Cryobacterium luteum sp.nov.,isolated from glacier ice.International Journal of Systematic and Evolutionary Microbiology,2012,62(6):1296-1299.
[22]Wilhelm RC,Niederberger TD,Greer C,Whyte LG.Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian high arctic.Canadian Journal of Microbiology,2011,57(4):303-315.
[23]Singh P,Singh SM,Singh RN,Naik S,Roy U,Srivastava A,B?lter M.Bacterial communities in ancient permafrost profiles of Svalbard,Arctic.Journal of Basic Microbiology,2017,57(12):1018-1036.
[24]Goordial J,Altshuler I,Hindson K,Chan-Yam K,Marcolefas E,Whyte LG.In situ field sequencing and life detection in remote(79°26′N)Canadian high arctic permafrost ice wedge microbial communities.Frontiers in Microbiology,2017,8:2594.
[25]Cai QH,Ye XD,Chen B,Zhang BY.Complete genome sequence of Exiguobacterium sp.strain N4-1P,a psychrophilic bioemulsifier producer isolated from a cold marine environment in north Atlantic Canada.Genome Announcements,2015,5(44):e01248-17.
[26]Kasana RC,Pandey CB.Exiguobacterium?:an overview of a versatile genus with potential in industry and agriculture.Critical Reviews in Biotechnology,2018,38(1):141-156.
[27]Castro-Severyn J,Remonsellez F,Valenzuela SL,Salinas C,Fortt J,Aguilar P,Pardo-EstéC,Dorador C,Quatrini R,Molina F,Aguayo D,Castro-Nallar E,Saavedra CP.Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of Stress-related genes and arsenic resistance.Frontiers in Microbiology,2017,8:456.
[28]Chaudhary DK,Kim J.Sphingopyxis nepalensis sp.nov.,isolated from oil-contaminated soil.International Journal of Systematic and Evolutionary Microbiology,2018,68(1):364-370.
[29]Verma H,Rani P,Kumar Singh A,Kumar R,Dwivedi V,Negi V,Lal R.Sphingopyxis flava sp.nov.,isolated from a hexachlorocyclohexane(HCH)-contaminated soil.International Journal of Systematic and Evolutionary Microbiology,2015,65(10):3720-3726.
[30]Oelschl?gel M,Rückert C,Kalinowski J,Schmidt G,Schl?mann M,Tischler D.Sphingopyxis fribergensis sp.nov.,a soil bacterium with the ability to degrade styrene and phenylacetic acid.International Journal of Systematic and Evolutionary Microbiology,2015,65(9):3008-3015.
[31]Nkem BM,Halimoon N,Yusoff FM,Johari WLW,Zakaria MP,Medipally SR,Kannan N.Isolation,identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach,Malaysia.Marine Pollution Bulletin,2016,107(1):261-268.
[32]Qin W,Fan FQ,Zhu Y,Huang XL,Ding AZ,Liu X,Dou JF.Anaerobic biodegradation of benzo(a)pyrene by a novel Cellulosimicrobium cellulans CWS2 isolated from polycyclic aromatic hydrocarbon-contaminated soil.Brazilian Journal of Microbiology,2018,49(2):258-268.
[33]Venil C,Nordin N,Zakaria Z,Ahmad W.Chryseobacterium artocarpi sp.nov.,isolated from the rhizosphere soil of Artocarpus integer.International Journal of Systematic and Evolutionary Microbiology,2014,64(9):3153-3159.
[34]Cai Y,Tao WZ,Ma YJ,Cheng J,Zhang MY,Zhang YX.Leifsonia flava sp.nov.,a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.Journal of Microbiology,2018,56(8):549-555.
[35]Pindi PK,Kishore KH,Reddy GSN,Shivaji S.Description of Leifsonia kafniensis sp.nov.and Leifsonia antarctica sp.nov.International Journal of Systematic and Evolutionary Microbiology,2009,59(6):1348-1352.
[36]Reddy GSN,Prakash JSS,Srinivas R,Matsumoto GI,Shivaji S.Leifsonia rubra sp.nov.and Leifsonia aurea sp.nov.,psychrophiles from a pond in Antarctica.International Journal of Systematic and Evolutionary Microbiology,2003,53(4):977-984.
[37]Kang SM,Asaf S,Kim SJ,Yun BW,Lee IJ.Complete genome sequence of plant growth-promoting bacterium Leifsonia xyli SE134,a possible gibberellin and auxin producer.Journal of Biotechnology,2016,239:34-38.
[38]Liu Y,Lai QL,Du J,Shao ZZ.Bacillus zhangzhouensis sp.nov.and Bacillus australimaris sp.nov.International Journal of Systematic and Evolutionary Microbiology,2016,66(3):1193-1199.
[39]Gos FMWR,Savi DC,Shaaban KA,Thorson JS,Aluizio R,Possiede YM,Rohr J,Glienke C.Antibacterial activity of endophytic actinomycetes isolated from the medicinal plant Vochysia divergens(Pantanal,Brazil).Frontiers in Microbiology,2017,8:1642.
[40]Gross H,Loper JE.Genomics of secondary metabolite production by Pseudomonas spp..Natural Product Reports,2009,26(11):1408-1446.
[41]Ghequire MGK,De Mot R.Ribosomally encoded antibacterial proteins and peptides from Pseudomonas.FEMS Microbiology Reviews,2014,38(4):523-568.
[42]Carrión VJ,van der Voort M,Arrebola E,Gutiérrez-Barranquero JA,de Vicente A,Raaijmakers JM,Cazorla FM.Mangotoxin production of Pseudomonas syringae pv.syringae is regulated by MgoA.BMCMicrobiology,2014,14:46.
[2]Deng HB,Lu LH,Bian LG.Surface radiation characteristics at Ny-?lesund over the arctic tundra area.Chinese Journal of Polar Research,2006,18(4):254-264.(in Chinese)邓海滨,陆龙骅,卞林根.北极苔原新奥尔松地区的地表辐射特征.极地研究,2006,18(4):254-264.
[3]Yao YF,Cao SN,Peng F,Li CS.Characteristics of species composition and community structure on the Austre Lovénbreen glacier foreland,Ny-?lesund,Svalbard.Chinese Journal of Polar Research,2014,26(3):362-368.(in Chinese)姚轶锋,曹叔楠,彭方,李承森.北极新奥尔松Austre Lovénbreen冰川退缩迹地不同演替阶段的植物组成与植被群落特征分析.极地研究,2014,26(3):362-368.
[4]Kim M,Jung JY,Laffly D,Kwon HY,Lee YK.Shifts in bacterial community structure during succession in a glacier foreland of the High Arctic.FEMS Microbiology Ecology,2017,93(1):fiw213.
[5]Neufeld JD,Mohn WW.Unexpectedly high bacterial diversity in arctic tundra relative to boreal forest soils,revealed by serial analysis of ribosomal sequence tags.Applied and Environmental Microbiology,2005,71(10):5710-5718.
[6]Peleg AY,Seifert H,Paterson DL.Acinetobacter baumannii:emergence of a successful pathogen.Clinical Microbiology Reviews,2008,21(3):538-582.
[7]Lee CR,Lee JH,Park M,Park KS,Bae IK,Kim YB,Cha CJ,Jeong BC,Lee SH.Biology of Acinetobacter baumannii:Pathogenesis,antibiotic resistance mechanisms,and prospective treatment options.Frontiers in cellular and infection microbiology,2017,7(55):1:35.
[8]Zhang XL,Li XH,Zhang F,Chen SC.Drug resistance analysis of staphylococcus aureus strains in Beijing Luhe Hospital in 2016.Medical Recapitulate,2018,24(2):409-412.(in Chinese)张晓兰,李晓辉,张芳,陈世财.医院2016年金黄色葡萄球菌耐药性分析.医学综述,2018,24(2):409-412.
[9]Kim MK,Park H,Oh TJ.Antibacterial and antioxidant capacity of polar microorganisms isolated from Arctic lichen Ochrolechia sp.Polish Journal of Microbiology,2014,63(3):317-322.
[10]Kim MK,Park H,Oh TJ.Antimicrobial properties of the bacterial associates of the Arctic lichen Stereocaulon sp.African Journal of Microbiology Research,2013,7:3651-3657.
[11]Liu QQ,Wang Y,Li J,Du ZJ,Chen GJ.Saccharicrinis carchari sp.nov.,isolated from a shark,and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans.International Journal of Systematic and Evolutionary Microbiology,2014,64(7):2204-2209.
[12]Nissinen RM,M?nnist?MK,van Elsas JD.Endophytic bacterial communities in three arctic plants from low arctic fell tundra are cold-adapted and host-plant specific.FEMSMicrobiology Ecology,2012,82(2):510-522.
[13]K?mpfer P,McInroy JA,Glaeser SP.Chryseobacterium zeae sp.nov.,Chryseobacterium arachidis sp.nov.,and Chryseobacterium geocarposphaerae sp.nov.isolated from the rhizosphere environment.Antonie van Leeuwenhoek,2014,105(3):491-500.
[14]K?mpfer P,McInroy JA,Glaeser SP.Chryseobacterium rhizoplanae sp.nov.,isolated from the rhizoplane environment.Antonie van Leeuwenhoek,2015,107(2):533-538.
[15]Du J,Ngo HTT,Won K,Kim KY,Jin FX,Yi TH.Chryseobacterium solani sp.nov.,isolated from field-grown eggplant rhizosphere soil.International Journal of Systematic and Evolutionary Microbiology,2015,65(8):2372-2377.
[16]Pot B,Willems A,Gillis M,de Ley J.Intra-and Intergeneric relationships of the genus Aquaspirillum:Prolinoborus,a New Genus for Aquaspirillum fasciculus,with the species Prolinoborus fasciculus comb.nov.International Journal of Systematic and Evolutionary Microbiology,1992,42(1):44-57.
[17]LabbéN,Parent S,Villemur R.Nitratireductor aquibiodomus gen.nov.,sp.nov.,a novelα-proteobacterium from the marine denitrification system of the Montreal Biodome(Canada).International Journal of Systematic and Evolutionary Microbiology,2004,54(1):269-273.
[18]Bajerski F,Ganzert L,Mangelsdorf K,Lipski A,Wagner D.Cryobacterium arcticum sp.nov.,a psychrotolerant bacterium from an arctic soil.International Journal of Systematic and Evolutionary Microbiology,2011,61(8):1849-1853.
[19]Reddy GSN,Pradhan S,Manorama R,Shivaji S.Cryobacterium roopkundense sp.nov.,a psychrophilic bacterium isolated from glacial soil.International Journal of Systematic and Evolutionary Microbiology,2010,60(4):866-870.
[20]Liu Q,Xin YH,Chen XL,Liu HC,Zhou YG,Chen WX.Cryobacterium aureum sp.nov.,a psychrophilic bacterium isolated from glacier ice collected from the ice tongue surface.International Journal of Systematic and Evolutionary Microbiology,2018,68(4):1173-1176.
[21]Liu Q,Liu HC,Wen Y,Zhou YG,Xin YH.Cryobacterium flavum sp.nov.and Cryobacterium luteum sp.nov.,isolated from glacier ice.International Journal of Systematic and Evolutionary Microbiology,2012,62(6):1296-1299.
[22]Wilhelm RC,Niederberger TD,Greer C,Whyte LG.Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian high arctic.Canadian Journal of Microbiology,2011,57(4):303-315.
[23]Singh P,Singh SM,Singh RN,Naik S,Roy U,Srivastava A,B?lter M.Bacterial communities in ancient permafrost profiles of Svalbard,Arctic.Journal of Basic Microbiology,2017,57(12):1018-1036.
[24]Goordial J,Altshuler I,Hindson K,Chan-Yam K,Marcolefas E,Whyte LG.In situ field sequencing and life detection in remote(79°26′N)Canadian high arctic permafrost ice wedge microbial communities.Frontiers in Microbiology,2017,8:2594.
[25]Cai QH,Ye XD,Chen B,Zhang BY.Complete genome sequence of Exiguobacterium sp.strain N4-1P,a psychrophilic bioemulsifier producer isolated from a cold marine environment in north Atlantic Canada.Genome Announcements,2015,5(44):e01248-17.
[26]Kasana RC,Pandey CB.Exiguobacterium?:an overview of a versatile genus with potential in industry and agriculture.Critical Reviews in Biotechnology,2018,38(1):141-156.
[27]Castro-Severyn J,Remonsellez F,Valenzuela SL,Salinas C,Fortt J,Aguilar P,Pardo-EstéC,Dorador C,Quatrini R,Molina F,Aguayo D,Castro-Nallar E,Saavedra CP.Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of Stress-related genes and arsenic resistance.Frontiers in Microbiology,2017,8:456.
[28]Chaudhary DK,Kim J.Sphingopyxis nepalensis sp.nov.,isolated from oil-contaminated soil.International Journal of Systematic and Evolutionary Microbiology,2018,68(1):364-370.
[29]Verma H,Rani P,Kumar Singh A,Kumar R,Dwivedi V,Negi V,Lal R.Sphingopyxis flava sp.nov.,isolated from a hexachlorocyclohexane(HCH)-contaminated soil.International Journal of Systematic and Evolutionary Microbiology,2015,65(10):3720-3726.
[30]Oelschl?gel M,Rückert C,Kalinowski J,Schmidt G,Schl?mann M,Tischler D.Sphingopyxis fribergensis sp.nov.,a soil bacterium with the ability to degrade styrene and phenylacetic acid.International Journal of Systematic and Evolutionary Microbiology,2015,65(9):3008-3015.
[31]Nkem BM,Halimoon N,Yusoff FM,Johari WLW,Zakaria MP,Medipally SR,Kannan N.Isolation,identification and diesel-oil biodegradation capacities of indigenous hydrocarbon-degrading strains of Cellulosimicrobium cellulans and Acinetobacter baumannii from tarball at Terengganu beach,Malaysia.Marine Pollution Bulletin,2016,107(1):261-268.
[32]Qin W,Fan FQ,Zhu Y,Huang XL,Ding AZ,Liu X,Dou JF.Anaerobic biodegradation of benzo(a)pyrene by a novel Cellulosimicrobium cellulans CWS2 isolated from polycyclic aromatic hydrocarbon-contaminated soil.Brazilian Journal of Microbiology,2018,49(2):258-268.
[33]Venil C,Nordin N,Zakaria Z,Ahmad W.Chryseobacterium artocarpi sp.nov.,isolated from the rhizosphere soil of Artocarpus integer.International Journal of Systematic and Evolutionary Microbiology,2014,64(9):3153-3159.
[34]Cai Y,Tao WZ,Ma YJ,Cheng J,Zhang MY,Zhang YX.Leifsonia flava sp.nov.,a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.Journal of Microbiology,2018,56(8):549-555.
[35]Pindi PK,Kishore KH,Reddy GSN,Shivaji S.Description of Leifsonia kafniensis sp.nov.and Leifsonia antarctica sp.nov.International Journal of Systematic and Evolutionary Microbiology,2009,59(6):1348-1352.
[36]Reddy GSN,Prakash JSS,Srinivas R,Matsumoto GI,Shivaji S.Leifsonia rubra sp.nov.and Leifsonia aurea sp.nov.,psychrophiles from a pond in Antarctica.International Journal of Systematic and Evolutionary Microbiology,2003,53(4):977-984.
[37]Kang SM,Asaf S,Kim SJ,Yun BW,Lee IJ.Complete genome sequence of plant growth-promoting bacterium Leifsonia xyli SE134,a possible gibberellin and auxin producer.Journal of Biotechnology,2016,239:34-38.
[38]Liu Y,Lai QL,Du J,Shao ZZ.Bacillus zhangzhouensis sp.nov.and Bacillus australimaris sp.nov.International Journal of Systematic and Evolutionary Microbiology,2016,66(3):1193-1199.
[39]Gos FMWR,Savi DC,Shaaban KA,Thorson JS,Aluizio R,Possiede YM,Rohr J,Glienke C.Antibacterial activity of endophytic actinomycetes isolated from the medicinal plant Vochysia divergens(Pantanal,Brazil).Frontiers in Microbiology,2017,8:1642.
[40]Gross H,Loper JE.Genomics of secondary metabolite production by Pseudomonas spp..Natural Product Reports,2009,26(11):1408-1446.
[41]Ghequire MGK,De Mot R.Ribosomally encoded antibacterial proteins and peptides from Pseudomonas.FEMS Microbiology Reviews,2014,38(4):523-568.
[42]Carrión VJ,van der Voort M,Arrebola E,Gutiérrez-Barranquero JA,de Vicente A,Raaijmakers JM,Cazorla FM.Mangotoxin production of Pseudomonas syringae pv.syringae is regulated by MgoA.BMCMicrobiology,2014,14:46.