Profile of Prof. Weizhi Ji
|
摘要
<正>Prof. Weizhi Ji is a distinguished scientist in nonhuman primate reproduction and developmental biology. He is devoted to uncovering the regulation mechanisms of early embryo development and stem cell pluripotency, and to understanding the pathogenesis of human diseases using nonhuman primate (NHP) models. Through decades of research,Prof. Ji has developed a series of research systems and technologies in NHPs including in vitro fertilization, early embryo culture, gene editing and stem cell isolation and
引文
Ai,Z.,Xiang,Z.,Li,Y.,Liu,G.,Wang,H.,Zheng,Y.,Qiu,X.,Zhao,S.,Zhu,X.,Li,Y.,et al.(2016).Conversion of monkey fibroblasts to transplantable telencephalic neuroepithelial stem cells.Biomaterials 77,53-65.
Chen,H.,Wei,Q.,Zhang,J.,Xu,C.,Tan,T.,and Ji,W.(2010).Netrin-1signaling mediates NO-induced glial precursor migration and accumulation.Cell Res 20,238-241.
Chen,X.,Li,T.,Li,X.,Xie,Y.,Guo,X.,Ji,S.,Niu,Y.,Yu,Y.,Ding,C.,Yao,R.,et al.(2009).Neural progenitors derived from monkey embryonic stem cells in a simple monoculture system.Reprod Biomed Online 19,426-433.
Chen,Y.,Wang,Z.,Xie,Y.,Guo,X.,Tang,X.,Wang,S.,Yang,S.,Chen,K.,Niu,Y.,and Ji,W.(2012).Folic acid deficiency inhibits neural rosette formation and neuronal differentiation from rhesus monkey embryonic stem cells.J Neurosci Res 90,1382-1391.
Chen,Y.,Niu,Y.,Li,Y.,Ai,Z.,Kang,Y.,Shi,H.,Xiang,Z.,Yang,Z.,Tan,T.,Si,W.,et al.(2015a).Generation of cynomolgus monkey chimeric fetuses using embryonic stem cells.Cell Stem Cell 17,116-124.
Chen,Y.,Zheng,Y.,Kang,Y.,Yang,W.,Niu,Y.,Guo,X.,Tu,Z.,Si,C.,Wang,H.,Xing,R.,et al.(2015b).Functional disruption of the dystrophin gene in rhesus monkey using CRISPR/Cas9.Human Mol Genet 24,3764-3774.
Chen,Y.,Yu,J.,Niu,Y.,Qin,D.,Liu,H.,Li,G.,Hu,Y.,Wang,J.,Lu,Y.,Kang,Y.,et al.(2017).Modeling rett syndrome using TALEN-edited MECP2 mutant cynomolgus monkeys.Cell 169,945-955.e10.
Cyranoski,D.(2016).Monkey kingdom.Nature 532,300-302.
Cui,Y.,Niu,Y.,Zhou,J.,Chen,Y.,Cheng,Y.,Li,S.,Ai,Z.,Chu,C.,Wang,H.,Zheng,B.,et al.(2018).Generation of a precise Oct4-hrGFPknockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination.Cell Res 28,383-386.
Gao,F.,Niu,Y.,Sun,Y.E.,Lu,H.,Chen,Y.,Li,S.,Kang,Y.,Luo,Y.,Si,C.,Yu,J.,et al.(2017).De novo DNA methylation during monkey preimplantation embryogenesis.Cell Res 27,526-539.
Jiang,B.,Xiang,Z.,Ai,Z.,Wang,H.,Li,Y.,Ji,W.,and Li,T.(2015).Generation of cardiac spheres from primate pluripotent stem cells in a small molecule-based 3D system.Biomaterials 65,103-114.
Jin,L.,Ji,S.,Tang,X.,Guo,X.,Lu,Y.,Chen,H.,Deng,H.,Zhou,Q.,and Ji,W.(2009).Isolation and characterization of liver epithelial progenitor cells from normal adult rhesus monkeys(Macaca mulatta).Cell Res 19,268-270.
Kang,Y.,Zheng,B.,Shen,B.,Chen,Y.,Wang,L.,Wang,J.,Niu,Y.,Cui,Y.,Zhou,J.,Wang,H.,et al.(2015).CRISPR/Cas9-mediated Dax1knockout in the monkey recapitulates human AHC-HH.Hum Mol Genet 24,7255-7264.
Wang,S.,Zhang,X.,Li,J.,Jin,L.,Ma,Y.,Wolf,D.P.,Zhou,Q.,and Ji,W.(2005).Transplantable neural progenitor populations derived from rhesus monkey embryonic stem cells.Stem Cell 10,2004-2008.
Li,T.,Wang,S.,Xie,Y.,Lu,Y.,Zhang,X.,Wang,L.,Yang,S.,Wolf,D.,Zhou,Q.,and Ji,W.(2005a).Homologous feeder cells support undifferentiated growth and pluripotency in monkey embryonic stem cells.Stem Cell 23,1192-1199.
Li,T.,Zheng,J.,Xie,Y.,Wang,S.,Zhang,X.,Li,J.,Jin,L.,Ma,Y.,Wolf,D.P.,Zhou,Q.,et al.(2005b).Transplantable neural progenitor populations derived from rhesus monkey embryonic stem cells.Stem Cell 23,1295-1303.
Liu,H.,Chen,Y.,Niu,Y.,Zhang,K.,Kang,Y.,Ge,W.,Liu,X.,Zhao,E.,Wang,C.,Lin,S.,et al.(2014).TALEN-mediated gene mutagenesis in rhesus and cynomolgus monkeys.Cell Stem Cell 14,323-328.
Niu,Y.,Yang,S.,Yu,Y.,Ding,C.,Yang,J.,Wang,S.,Ji,S.,He,X.,Xie,Y.,Tang,X.,et al.(2008).Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos.Cloning Stem Cells 10,25-36.
Niu,Y.,Yu,Y.,Bernat,A.,Yang,S.,He,X.,Guo,X.,Chen,D.,Chen,Y.,Ji,S.,Si,W.,et al.(2010).Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector.Proc Natl Acad Sci USA 107,17663-17667.
Niu,Y.,Shen,B.,Cui,Y.,Chen,Y.,Wang,J.,Wang,L.,Kang,Y.,Zhao,X.,Si,W.,Li,W.,et al.(2014).Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos.Cell 156,836-843.
Niu,Y.,Guo,X.,Chen,Y.,Wang,C.,Gao,J.,Yang,W.,Kang,Y.,Si,W.,Wang,H.,Yang,S.,et al.(2015).Early Parkinson's disease symptoms inα-synuclein transgenic monkeys.Human Mol Genet 24,2308-2317.
Si,W.,Zheng,P.,Tang,X.,He,X.,Wang,H.,Bavister,B.D.,and Ji,W.(2000).Cryopreservation of rhesus macaque(Macaca mulatta)spermatozoa and their functional assessment by in vitro fertilization.Cryobiology 41,232-240.
Wang,H.,Tan,T.,Wang,J.,Niu,Y.,Yan,Y.,Guo,X.,Kang,Y.,Duan,Y.,Chang,S.,Liao,J.,et al.(2015).Rhesus monkey model of liver disease reflecting clinical disease progression and hepatic gene expression analysis.Sci Rep 5,15019.
Wang,S.,Tang,X.,Niu,Y.,Chen,H.,Li,B.,Li,T.,Zhang,X.,Hu,Z.,Zhou,Q.,and Ji,W.(2007).Generation and characterization of rabbit embryonic stem cells.Stem Cell 25,481-489.
Wang,S.,Shen,Y.,Yuan,X.,Chen,K.,Guo,X.,Chen,Y.,Niu,Y.,Li,J.,Xu,R.,Yan,X.,et al.(2008).Dissecting signaling pathways that govern self-renewal of rabbit embryonic stem cells.J Biol Chem 283,35929-35940.
Yang,J.,Yang,S.,Beaujean,N.,Niu,Y.,He,X.,Xie,Y.,Tang,X.,Wang,L.,Zhou,Q.,and Ji,W.(2006).Epigenetic marks in cloned rhesus monkey embryos:comparison with counterparts produced in vitro.Biol Reprod 76,36-42.
Yang,S.,He,X.,Hildebrandt,T.B.,Zhou,Q.,and Ji,W.(2007).Super ovulatory response to a low dose single-daily treatment of rhFSHdissolved in polyvinylpyrrolidone in rhesus monkeys.Am J Primatol69,1278-1284.
Yu,Y.,Ding,C.,Wang,E.,Chen,X.,Li,X.,Zhao,C.,Fan,Y.,Wang,L.,Beaujean,N.,Zhou,Q.,et al.(2007).Piezo-assisted nuclear transfer affects cloning efficiency and may cause apoptosis.Reproduction 133,947-954.
Zheng,P.(2001a).Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development.Human Reprod 16,300-305.
Zheng,P.,Bavister,B.D.,and Ji,W.(2001b).Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys.Mol Reprod Dev 58,348-355.
Zheng,P.,Bavister,B.,and Ji,W.(2002).Amino acid requirements for maturation of rhesus monkey(Macacca mulatta)oocytes in culture.Reproduction 124,515-522.
Zheng,P.(2003).17-Estradiol and progesterone improve in vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys.Human Reprod 18,2137-2144.
Zhou,Q.,Yang,S.,Ding,C.,He,X.,Xie,Y.,Hildebrandt,T.B.,Mitalipov,S.M.,Tang,X.,Wolf,D.P.,and Ji,W.(2006).A comparative approach to somatic cell nuclear transfer in the rhesus monkey.Human Reprod21,2564-2571.
Zhu,X.,Li,B.,Ai,Z.,Xiang,Z.,Zhang,K.,Qiu,X.,Chen,Y.,Li,Y.,Rizak,J.D.,Niu,Y.,et al.(2016).A robust single primate neuroepithelial cell clonal expansion system for neural tube development and disease studies.Stem Cell Rep 6,228-242.
Chen,H.,Wei,Q.,Zhang,J.,Xu,C.,Tan,T.,and Ji,W.(2010).Netrin-1signaling mediates NO-induced glial precursor migration and accumulation.Cell Res 20,238-241.
Chen,X.,Li,T.,Li,X.,Xie,Y.,Guo,X.,Ji,S.,Niu,Y.,Yu,Y.,Ding,C.,Yao,R.,et al.(2009).Neural progenitors derived from monkey embryonic stem cells in a simple monoculture system.Reprod Biomed Online 19,426-433.
Chen,Y.,Wang,Z.,Xie,Y.,Guo,X.,Tang,X.,Wang,S.,Yang,S.,Chen,K.,Niu,Y.,and Ji,W.(2012).Folic acid deficiency inhibits neural rosette formation and neuronal differentiation from rhesus monkey embryonic stem cells.J Neurosci Res 90,1382-1391.
Chen,Y.,Niu,Y.,Li,Y.,Ai,Z.,Kang,Y.,Shi,H.,Xiang,Z.,Yang,Z.,Tan,T.,Si,W.,et al.(2015a).Generation of cynomolgus monkey chimeric fetuses using embryonic stem cells.Cell Stem Cell 17,116-124.
Chen,Y.,Zheng,Y.,Kang,Y.,Yang,W.,Niu,Y.,Guo,X.,Tu,Z.,Si,C.,Wang,H.,Xing,R.,et al.(2015b).Functional disruption of the dystrophin gene in rhesus monkey using CRISPR/Cas9.Human Mol Genet 24,3764-3774.
Chen,Y.,Yu,J.,Niu,Y.,Qin,D.,Liu,H.,Li,G.,Hu,Y.,Wang,J.,Lu,Y.,Kang,Y.,et al.(2017).Modeling rett syndrome using TALEN-edited MECP2 mutant cynomolgus monkeys.Cell 169,945-955.e10.
Cyranoski,D.(2016).Monkey kingdom.Nature 532,300-302.
Cui,Y.,Niu,Y.,Zhou,J.,Chen,Y.,Cheng,Y.,Li,S.,Ai,Z.,Chu,C.,Wang,H.,Zheng,B.,et al.(2018).Generation of a precise Oct4-hrGFPknockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination.Cell Res 28,383-386.
Gao,F.,Niu,Y.,Sun,Y.E.,Lu,H.,Chen,Y.,Li,S.,Kang,Y.,Luo,Y.,Si,C.,Yu,J.,et al.(2017).De novo DNA methylation during monkey preimplantation embryogenesis.Cell Res 27,526-539.
Jiang,B.,Xiang,Z.,Ai,Z.,Wang,H.,Li,Y.,Ji,W.,and Li,T.(2015).Generation of cardiac spheres from primate pluripotent stem cells in a small molecule-based 3D system.Biomaterials 65,103-114.
Jin,L.,Ji,S.,Tang,X.,Guo,X.,Lu,Y.,Chen,H.,Deng,H.,Zhou,Q.,and Ji,W.(2009).Isolation and characterization of liver epithelial progenitor cells from normal adult rhesus monkeys(Macaca mulatta).Cell Res 19,268-270.
Kang,Y.,Zheng,B.,Shen,B.,Chen,Y.,Wang,L.,Wang,J.,Niu,Y.,Cui,Y.,Zhou,J.,Wang,H.,et al.(2015).CRISPR/Cas9-mediated Dax1knockout in the monkey recapitulates human AHC-HH.Hum Mol Genet 24,7255-7264.
Wang,S.,Zhang,X.,Li,J.,Jin,L.,Ma,Y.,Wolf,D.P.,Zhou,Q.,and Ji,W.(2005).Transplantable neural progenitor populations derived from rhesus monkey embryonic stem cells.Stem Cell 10,2004-2008.
Li,T.,Wang,S.,Xie,Y.,Lu,Y.,Zhang,X.,Wang,L.,Yang,S.,Wolf,D.,Zhou,Q.,and Ji,W.(2005a).Homologous feeder cells support undifferentiated growth and pluripotency in monkey embryonic stem cells.Stem Cell 23,1192-1199.
Li,T.,Zheng,J.,Xie,Y.,Wang,S.,Zhang,X.,Li,J.,Jin,L.,Ma,Y.,Wolf,D.P.,Zhou,Q.,et al.(2005b).Transplantable neural progenitor populations derived from rhesus monkey embryonic stem cells.Stem Cell 23,1295-1303.
Liu,H.,Chen,Y.,Niu,Y.,Zhang,K.,Kang,Y.,Ge,W.,Liu,X.,Zhao,E.,Wang,C.,Lin,S.,et al.(2014).TALEN-mediated gene mutagenesis in rhesus and cynomolgus monkeys.Cell Stem Cell 14,323-328.
Niu,Y.,Yang,S.,Yu,Y.,Ding,C.,Yang,J.,Wang,S.,Ji,S.,He,X.,Xie,Y.,Tang,X.,et al.(2008).Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos.Cloning Stem Cells 10,25-36.
Niu,Y.,Yu,Y.,Bernat,A.,Yang,S.,He,X.,Guo,X.,Chen,D.,Chen,Y.,Ji,S.,Si,W.,et al.(2010).Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector.Proc Natl Acad Sci USA 107,17663-17667.
Niu,Y.,Shen,B.,Cui,Y.,Chen,Y.,Wang,J.,Wang,L.,Kang,Y.,Zhao,X.,Si,W.,Li,W.,et al.(2014).Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos.Cell 156,836-843.
Niu,Y.,Guo,X.,Chen,Y.,Wang,C.,Gao,J.,Yang,W.,Kang,Y.,Si,W.,Wang,H.,Yang,S.,et al.(2015).Early Parkinson's disease symptoms inα-synuclein transgenic monkeys.Human Mol Genet 24,2308-2317.
Si,W.,Zheng,P.,Tang,X.,He,X.,Wang,H.,Bavister,B.D.,and Ji,W.(2000).Cryopreservation of rhesus macaque(Macaca mulatta)spermatozoa and their functional assessment by in vitro fertilization.Cryobiology 41,232-240.
Wang,H.,Tan,T.,Wang,J.,Niu,Y.,Yan,Y.,Guo,X.,Kang,Y.,Duan,Y.,Chang,S.,Liao,J.,et al.(2015).Rhesus monkey model of liver disease reflecting clinical disease progression and hepatic gene expression analysis.Sci Rep 5,15019.
Wang,S.,Tang,X.,Niu,Y.,Chen,H.,Li,B.,Li,T.,Zhang,X.,Hu,Z.,Zhou,Q.,and Ji,W.(2007).Generation and characterization of rabbit embryonic stem cells.Stem Cell 25,481-489.
Wang,S.,Shen,Y.,Yuan,X.,Chen,K.,Guo,X.,Chen,Y.,Niu,Y.,Li,J.,Xu,R.,Yan,X.,et al.(2008).Dissecting signaling pathways that govern self-renewal of rabbit embryonic stem cells.J Biol Chem 283,35929-35940.
Yang,J.,Yang,S.,Beaujean,N.,Niu,Y.,He,X.,Xie,Y.,Tang,X.,Wang,L.,Zhou,Q.,and Ji,W.(2006).Epigenetic marks in cloned rhesus monkey embryos:comparison with counterparts produced in vitro.Biol Reprod 76,36-42.
Yang,S.,He,X.,Hildebrandt,T.B.,Zhou,Q.,and Ji,W.(2007).Super ovulatory response to a low dose single-daily treatment of rhFSHdissolved in polyvinylpyrrolidone in rhesus monkeys.Am J Primatol69,1278-1284.
Yu,Y.,Ding,C.,Wang,E.,Chen,X.,Li,X.,Zhao,C.,Fan,Y.,Wang,L.,Beaujean,N.,Zhou,Q.,et al.(2007).Piezo-assisted nuclear transfer affects cloning efficiency and may cause apoptosis.Reproduction 133,947-954.
Zheng,P.(2001a).Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development.Human Reprod 16,300-305.
Zheng,P.,Bavister,B.D.,and Ji,W.(2001b).Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys.Mol Reprod Dev 58,348-355.
Zheng,P.,Bavister,B.,and Ji,W.(2002).Amino acid requirements for maturation of rhesus monkey(Macacca mulatta)oocytes in culture.Reproduction 124,515-522.
Zheng,P.(2003).17-Estradiol and progesterone improve in vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys.Human Reprod 18,2137-2144.
Zhou,Q.,Yang,S.,Ding,C.,He,X.,Xie,Y.,Hildebrandt,T.B.,Mitalipov,S.M.,Tang,X.,Wolf,D.P.,and Ji,W.(2006).A comparative approach to somatic cell nuclear transfer in the rhesus monkey.Human Reprod21,2564-2571.
Zhu,X.,Li,B.,Ai,Z.,Xiang,Z.,Zhang,K.,Qiu,X.,Chen,Y.,Li,Y.,Rizak,J.D.,Niu,Y.,et al.(2016).A robust single primate neuroepithelial cell clonal expansion system for neural tube development and disease studies.Stem Cell Rep 6,228-242.