脚板薯的组织培养与快速繁殖研究
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摘要
为建立一套完整的脚板薯离体快速繁殖体系,以脚板薯茎段为试验材料,研究脚板薯茎段表面灭菌、丛生芽诱导、无菌苗增殖及移栽等。结果表明,脚板薯茎段丛生芽诱导的最佳培养基为MS+NAA 0.4 mg/L+6-BA 3.2 mg/L+烯效唑2.9 mg/L+活性炭1.2 g/L,诱导率为93.6%;丛生芽增殖的最佳培养基为MS+NAA 0.7 mg/L+6-BA 0.8 mg/L+烯效唑2.9 mg/L+活性炭1.6 g/L+蔗糖40 g/L,增殖倍数为4.61,组培苗炼苗成活率达70%以上。本研究建立了脚板薯的离体快速繁殖技术体系,研究结果可为脚板薯的工业化育苗提供技术参考。
引文
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[6]叶文峰,周秀玲. 超声波-酸解法制备脚板薯抗性淀粉的工艺条件[J]. 江苏农业科学,2015,43(7):281-283.
[7]Oh P S,Lim K T. Glycoprotein isolated from Dioscorea batatas Decne modulates expressions of IL-4 and IL-10 in primary-cultured mouse lymphocytes[J]. Cell biochemistry and function,2009,27(5):316-322.
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[9]Kim S,Jwa H,Yanagawa Y,et al. Extract from Dioscorea batatas ameliorates insulin resistance in mice fed a high-fat diet[J]. Journal of Medicinal Food,2012,15(6):527.
[10]娄丽娜,刘哲,许园园,等. 萝卜与芜菁异源三倍体杂种的获得及鉴定[J]. 江苏农业学报,2017,33(4):881-889.
[11]Malaurie B,Pungu O,Trouslot M F. Effect of growth regulators concentrations on morphological development of meristem-tips in Dioscorea cayenensis-D.rotundata complex and D. praehensilis[J]. Plant cell,tissue and organ culture,1995,41 (3):229-235.
[12]Jasik J,Mantell S. Effects of jasmonic acid and its methylester on in vitro microtuberisation of three food yam (Dioscorea) species[J]. Plant Cell Reports,2000,19 (9):863-867.
[13]Alizadeh S,Mantell S H,Mariaviana A. In vitro shoot culture and microtuber induction in the steroid yam Dioscorea composita Hemsl[J]. Plant Cell,Tissue and Organ Culture,1998,53 (2):107-112.
[14]王应想,杨薇,杨柏云,等. 脚板薯脱毒试管苗的快繁及零余子的诱导[C]. 第二届全国植物组织培养、脱毒快繁及工厂化生产学术研讨会,2004.
[2]Duan Y,Kim G H,Kim H S. Study on physiologically active compounds and antioxidant activity of Korean yam (Dioscorea batatas Decne.)[J]. J of Korean Oil Chemists' Soc,2016,33(2):342-351.
[3]Jin M,Suh S J,Yang J H,et al. Anti-inflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-κB and ERK1/2 inactivation[J]. Food and Chemical Toxicology,2010,48 (11):3073-3079.
[4]Kang M K,Kim J S,Kim G C,et al. Quality characteristics of pancake premix with Dioscorea batatas powder by steaming process[J]. Korean Journal of Food and Cookery Science,2016,32(5):593-599.
[5]Kim S,Shin M Y,Son K H,et al. Yam (Dioscorea batatas) root and bark extracts stimulate osteoblast mineralization by increasing Ca and P accumulation and alkaline phosphatase activity[J]. Prev Nutr Food Sci,2014,19(3):194-203.
[6]叶文峰,周秀玲. 超声波-酸解法制备脚板薯抗性淀粉的工艺条件[J]. 江苏农业科学,2015,43(7):281-283.
[7]Oh P S,Lim K T. Glycoprotein isolated from Dioscorea batatas Decne modulates expressions of IL-4 and IL-10 in primary-cultured mouse lymphocytes[J]. Cell biochemistry and function,2009,27(5):316-322.
[8]Choi E M,Koo S J,Hwang J-K. Immune cell stimulating activity of mucopolysaccharide isolated from yam (Dioscorea batatas)[J]. Journal of ethnopharmacology,2004,91 (1):1-6.
[9]Kim S,Jwa H,Yanagawa Y,et al. Extract from Dioscorea batatas ameliorates insulin resistance in mice fed a high-fat diet[J]. Journal of Medicinal Food,2012,15(6):527.
[10]娄丽娜,刘哲,许园园,等. 萝卜与芜菁异源三倍体杂种的获得及鉴定[J]. 江苏农业学报,2017,33(4):881-889.
[11]Malaurie B,Pungu O,Trouslot M F. Effect of growth regulators concentrations on morphological development of meristem-tips in Dioscorea cayenensis-D.rotundata complex and D. praehensilis[J]. Plant cell,tissue and organ culture,1995,41 (3):229-235.
[12]Jasik J,Mantell S. Effects of jasmonic acid and its methylester on in vitro microtuberisation of three food yam (Dioscorea) species[J]. Plant Cell Reports,2000,19 (9):863-867.
[13]Alizadeh S,Mantell S H,Mariaviana A. In vitro shoot culture and microtuber induction in the steroid yam Dioscorea composita Hemsl[J]. Plant Cell,Tissue and Organ Culture,1998,53 (2):107-112.
[14]王应想,杨薇,杨柏云,等. 脚板薯脱毒试管苗的快繁及零余子的诱导[C]. 第二届全国植物组织培养、脱毒快繁及工厂化生产学术研讨会,2004.