重庆武隆高山甘蓝根肿病气象预测研究
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摘要
为防御和减轻重庆地区高山甘蓝根肿病的发生,选择武隆高山甘蓝为研究对象,采用该区2010—2014年气象资料和高山甘蓝田间试验资料,分析气象因子与根肿病发病率的关系。筛选出对根肿病影响显著的气象因子为定植—成活期的累积雨量、定植后第12～14日平均气温,采用回归方法建立甘蓝根肿病气象预测模型。利用2015—2016年试验资料对模型进行验证和试用,确定系数R2为0.68,说明该预测方法具有较好的客观性和适用性,可应用于重庆高山地区甘蓝根肿病发生的气象条件预测。气象防治和生物防治相结合,将为高山甘蓝根肿病的综合防治提供新的理论依据。
To prevent and reduce the occurrence of clubroot in mountainous areas of Chongqing, the cabbage of Wulong district was selected as the research object. The relationship between the meteorological factors and the incidence of clubroot was analyzed by using meteorological data of 2010-2014 and field examination data of mountain cabbage in this area. The accumulated precipitation at the transplanting survival period and the mean temperature at 12-14 days after field planting were used to establish a meteorological prediction model for cabbage clubroot. The regression model was verified and tested by 2015-2016 examination data, the coefficient of determination R2 was 0.68. It proves that the prediction method has good objectivity and applicability, and can be applied to the forecast of meteorological conditions of the occurrence of cabbage clubroot in Chongqing mountainous areas. The combination of meteorological control and biological control will provide a new theoretical basis for the integrated control of clubroot in mountainous areas.
To prevent and reduce the occurrence of clubroot in mountainous areas of Chongqing, the cabbage of Wulong district was selected as the research object. The relationship between the meteorological factors and the incidence of clubroot was analyzed by using meteorological data of 2010-2014 and field examination data of mountain cabbage in this area. The accumulated precipitation at the transplanting survival period and the mean temperature at 12-14 days after field planting were used to establish a meteorological prediction model for cabbage clubroot. The regression model was verified and tested by 2015-2016 examination data, the coefficient of determination R2 was 0.68. It proves that the prediction method has good objectivity and applicability, and can be applied to the forecast of meteorological conditions of the occurrence of cabbage clubroot in Chongqing mountainous areas. The combination of meteorological control and biological control will provide a new theoretical basis for the integrated control of clubroot in mountainous areas.
引文
[1] Dixon G R. The biology of Plasmodiophora brassicae Wor.-are view of recent advances[J]. Acta Horticulturea,2006(6):271-282.
[2]孙保亚,沈向群,郭海峰.十字花科植物根肿病及抗病育种研究进展[J].中国蔬菜,2005(4):34-37
[3]章元寿.植物病理生理学[M].南京:江苏科学技术出版社,1993:43.
[4]杨字红,邹立君,张雄飞.大白菜根肿病的药剂防治[J].中国蔬菜,1994(3):18-20.
[5]刘亚培,王芳展,胡帅,等.十字花科蔬菜根肿病及其综合防治技术[J].浙江农业科学,2012(5):694-697.
[6]卢晓霞,康晓慧,付菊梅,等.油菜根肿病对油菜光合作用及酶活性的影响[J],广东农业科学,2013(24):71-73.
[7]王靖,黄云,李小兰,等.十字花科根肿病研究进展[J].植物保护,2011,37(6):153-158.
[8]唐文华.北京十字花科蔬菜根肿病的发生和鉴定[J].植物保护,1990,16(1):17-18.
[9]李晓敏.武隆县白菜根肿病的发生原因及防治措施[J].南方农业,2011,5(11):33-34.
[10] Bradshaw J E, Dorothy J G, Wilson R N. Transfer of resistamce to clubroot(Plasmodiophora brassicae)to swedes(Brassica napus L.var. napobrassica Peterm)from B. rapa[J]. Annals of applied biology,1997,130(2):337-348.
[11] Manzanares-Dauleux M J, Delourme R, Baron F, et al. Mapping of one major gene and of QTLs involved in resistance to clubroot in Brassica napus[J]. Theoretical and Applied Genetics,2000,101(5-6):885-891.
[12]符明联,杨玉珠,李根泽,等.不同油菜品种对根肿病的抗性分析[J].华中农业大学报,2011,30(4):443-447.
[13]谭翀,岳艳玲.不同大白菜品种对根肿病的抗性鉴定[J].中国蔬菜,200(4):91-94.
[14]胡靖锋,吴丽艳,林良斌,等.用菌±接种法鉴定云南省主要十字花科作物对根肿病的抗性[J].中国蔬菜,2010(14):71-74.
[15] Lahlali R, Peng G, Mc Gregor L, et al. Mechanisms of the biofungicide Serenade(Bacillus subtilis QST713)in suppressing clubroot[J]. Biocontrol Science and Technology,2011,21(11):1351-1362.
[16] Wang Jing, Huang Yun, Lin Shan, et al. A strain of Streptomyces griseoruber isolated from rhizospheric soil of Chinese cabbage as antagonist to Plasmodiophora brassicae[J]. Annals of microbiology,2012,62(1):247-253.
[17] Narisawa K, Tokumasu S. Hashiba Suppression of clubroot formation in Chinese cabbage by the root endophytic fungus,Heteroconium chaetospira[J]. Plant Pathology,1998,47(2):206-210.
[18] Guo Shengye, Li Xingyu, He Pengfei, et al. Whole-genome sequencing of Bacillus subtilis XF-1 reveals mechanisms for biological control and multiple beneficial properties in plants[J]. J Ind Microbiol Biotechnol,2015,42:925-937.
[19] Lee S O, Choi G J, Choi Y H, et al. Isolation and characterization of endophytic actinomycetes from Chinese cabbage roots as antagonists to Plasmodiophora brassicae[J]. Journal of Microbiology&Biotechnology,2008,18(11):1741-1746.
[20]王会军,陈卓君,吴毅献,等.防治十字花科作物根肿病的油菜内生细菌分离与鉴定[J].中国油料作物学报,2014,36(1):92-97.
[21]肖崇刚,郭向华.甘蓝根肿病菌的生物学特性研究[J].菌物系统,2002,21(4):597-603.
[22]黄云,马淑青,李晓琴,等.油菜根肿病菌的形态和休眠孢子的生物学特性[J].中国农业科学,2007,40(7):1388-1394.
[23] Murakami H, Tsushima S, Shishido Y. Factors affecting the pattern of the dose response curve of clubroot disease caused by Plasmodiophora brassicae[J]. Soil Science and Plant Nutrition,2002,48(3):421-427.
[24] Sharmaa K, Gossenb B D, McDonalda M R. Effect of temperature on primary infection by Plasmodiophora brassicae and initiation of clubroot symptoms[J]. Plant Pathology,2011,60(5):830-838.
[25] Webster. pH and nutritional effects on infection by Plasmodiophora brassicae Wor. and on clubroot symptoms[D]. University of Aberdeen,1986.
[26] Sharmaa K, Gossenb B D, McDonalda M R. Effect of temperature on cortical infection by Plasmodiophora brassicae and clubroot severity[J]. Phytopathology,2011,101(12):1424-1432.
[27]李惠明,潘月华,沈明龙.十字花科根肿病发生规律及测报防治技术[J].长江蔬菜,1999(9):15-16.
[28]余仕金.十字花科作物根肿病发生规律及防治研究[J].云南农业,2009(3):45-46.
[29]王旭炜,彭洪江,高明泉,等.茎瘤芥(榨菜)根肿病病源初步鉴定及发病影响因素[J].西南农业学报,2002,15(4):75-81.
[30]杨明英,李向东,孙道旺,等.白菜根肿病菌致病性因素研究[J].西南农业大学学报,2011,24(2):612-615.
[31]余汉清,华秀丽.大白菜根肿病发生条件与防治[J].长江蔬菜,1996(10):18-19.
[32] Buczacki S T, Ockendon J G, Free-man G H. An analysis of some effects of light and soil temperature on club root disease[J]. Ann Appl Biol,1978,88:229-238.
[33]尹全,宋君,刘勇,等.土壤根肿病菌休眠孢子PCR快速检测方法的建立[J].西南农业学报,2010,23(2):390-392.
[34]杨家莺,孙道旺,杨明英,等.75%达克宁防治白莱根肿病产量损失测定及残留分析[J].西南农业学报,2002,17(2):189-191.
[35]杨佩文,尚慧,董丽英,等.大白菜根肿病化学防治技术[J].植物保护,2009,35(6):157-159.
[36]王贵斌,王新生,卢惠芝,等.氰霜唑预防大白菜根肿效果好[J].农药市场信息,2012,28:41.
[37] Hiroharu Murakami, Seiya Tsushima, Yuldko Kuroyanagi, et al.Reduction of resting spore density of Plasmodiophora brasicae and clubroot disease severity by lining[J]. Soil science and plant nutrition.2002,48(5):685-691.
[38]戴玉华,杨莲,李顺德,等.调节土壤p H防治白菜根肿病技术探索[J].中国植保导刊,2004,24(8):20-22.
[39]李壮,牛应泽,吴永成,等.根肿病的研究进展[J].西南农业学报,2013(4):1733-1737.
[40] Buczacki S T, Ockendon J G, Freeman G H. An analysis of some effects of light and soil temperature on clubroot disease[J]. Ann Appl Biol,1978(88):229-238.
[41] Buczacki T, Moxham S E. Structure of the resting spore wall of Plasmodiophora brassicae revealed by electron microscopy and chemical digestion[J]. Transactions of the British Mycological Society,1983,81(2):221-231
[42]郭向华.甘蓝根肿病菌的生物学特性及致病研究[D].重庆:西南农业大学,2001.
[43] Dixon G R. Variation in Plasmodiophora brassicae[J]. Annals of Applied Biology,1980,94(2):278-280
[44]马淑青,黄云,王铮一,等.油菜根肿病菌形态及生物学特性研究[J].四川农业大学学报,2006(2):161-165.
[45] Macfarlane I. Germination of resting spores of Plasmodiophora brassicae[J]. Transactions of the British Mycological Society,1970,55(1):97-112.
[46]李明远.十字花科根肿病的发生及防治[J].北京农业科学,1990,8(4):11-13.
[47]谢文瑞.十字花科蔬菜根瘤病菌之生态[A].作物绝对寄生真菌病害研讨会专刊[C].1992:145-146.
[48]吕理燊.十字花科蔬菜根肿病[J].云南农业大学学报,2002,17(2):134-136.
[2]孙保亚,沈向群,郭海峰.十字花科植物根肿病及抗病育种研究进展[J].中国蔬菜,2005(4):34-37
[3]章元寿.植物病理生理学[M].南京:江苏科学技术出版社,1993:43.
[4]杨字红,邹立君,张雄飞.大白菜根肿病的药剂防治[J].中国蔬菜,1994(3):18-20.
[5]刘亚培,王芳展,胡帅,等.十字花科蔬菜根肿病及其综合防治技术[J].浙江农业科学,2012(5):694-697.
[6]卢晓霞,康晓慧,付菊梅,等.油菜根肿病对油菜光合作用及酶活性的影响[J],广东农业科学,2013(24):71-73.
[7]王靖,黄云,李小兰,等.十字花科根肿病研究进展[J].植物保护,2011,37(6):153-158.
[8]唐文华.北京十字花科蔬菜根肿病的发生和鉴定[J].植物保护,1990,16(1):17-18.
[9]李晓敏.武隆县白菜根肿病的发生原因及防治措施[J].南方农业,2011,5(11):33-34.
[10] Bradshaw J E, Dorothy J G, Wilson R N. Transfer of resistamce to clubroot(Plasmodiophora brassicae)to swedes(Brassica napus L.var. napobrassica Peterm)from B. rapa[J]. Annals of applied biology,1997,130(2):337-348.
[11] Manzanares-Dauleux M J, Delourme R, Baron F, et al. Mapping of one major gene and of QTLs involved in resistance to clubroot in Brassica napus[J]. Theoretical and Applied Genetics,2000,101(5-6):885-891.
[12]符明联,杨玉珠,李根泽,等.不同油菜品种对根肿病的抗性分析[J].华中农业大学报,2011,30(4):443-447.
[13]谭翀,岳艳玲.不同大白菜品种对根肿病的抗性鉴定[J].中国蔬菜,200(4):91-94.
[14]胡靖锋,吴丽艳,林良斌,等.用菌±接种法鉴定云南省主要十字花科作物对根肿病的抗性[J].中国蔬菜,2010(14):71-74.
[15] Lahlali R, Peng G, Mc Gregor L, et al. Mechanisms of the biofungicide Serenade(Bacillus subtilis QST713)in suppressing clubroot[J]. Biocontrol Science and Technology,2011,21(11):1351-1362.
[16] Wang Jing, Huang Yun, Lin Shan, et al. A strain of Streptomyces griseoruber isolated from rhizospheric soil of Chinese cabbage as antagonist to Plasmodiophora brassicae[J]. Annals of microbiology,2012,62(1):247-253.
[17] Narisawa K, Tokumasu S. Hashiba Suppression of clubroot formation in Chinese cabbage by the root endophytic fungus,Heteroconium chaetospira[J]. Plant Pathology,1998,47(2):206-210.
[18] Guo Shengye, Li Xingyu, He Pengfei, et al. Whole-genome sequencing of Bacillus subtilis XF-1 reveals mechanisms for biological control and multiple beneficial properties in plants[J]. J Ind Microbiol Biotechnol,2015,42:925-937.
[19] Lee S O, Choi G J, Choi Y H, et al. Isolation and characterization of endophytic actinomycetes from Chinese cabbage roots as antagonists to Plasmodiophora brassicae[J]. Journal of Microbiology&Biotechnology,2008,18(11):1741-1746.
[20]王会军,陈卓君,吴毅献,等.防治十字花科作物根肿病的油菜内生细菌分离与鉴定[J].中国油料作物学报,2014,36(1):92-97.
[21]肖崇刚,郭向华.甘蓝根肿病菌的生物学特性研究[J].菌物系统,2002,21(4):597-603.
[22]黄云,马淑青,李晓琴,等.油菜根肿病菌的形态和休眠孢子的生物学特性[J].中国农业科学,2007,40(7):1388-1394.
[23] Murakami H, Tsushima S, Shishido Y. Factors affecting the pattern of the dose response curve of clubroot disease caused by Plasmodiophora brassicae[J]. Soil Science and Plant Nutrition,2002,48(3):421-427.
[24] Sharmaa K, Gossenb B D, McDonalda M R. Effect of temperature on primary infection by Plasmodiophora brassicae and initiation of clubroot symptoms[J]. Plant Pathology,2011,60(5):830-838.
[25] Webster. pH and nutritional effects on infection by Plasmodiophora brassicae Wor. and on clubroot symptoms[D]. University of Aberdeen,1986.
[26] Sharmaa K, Gossenb B D, McDonalda M R. Effect of temperature on cortical infection by Plasmodiophora brassicae and clubroot severity[J]. Phytopathology,2011,101(12):1424-1432.
[27]李惠明,潘月华,沈明龙.十字花科根肿病发生规律及测报防治技术[J].长江蔬菜,1999(9):15-16.
[28]余仕金.十字花科作物根肿病发生规律及防治研究[J].云南农业,2009(3):45-46.
[29]王旭炜,彭洪江,高明泉,等.茎瘤芥(榨菜)根肿病病源初步鉴定及发病影响因素[J].西南农业学报,2002,15(4):75-81.
[30]杨明英,李向东,孙道旺,等.白菜根肿病菌致病性因素研究[J].西南农业大学学报,2011,24(2):612-615.
[31]余汉清,华秀丽.大白菜根肿病发生条件与防治[J].长江蔬菜,1996(10):18-19.
[32] Buczacki S T, Ockendon J G, Free-man G H. An analysis of some effects of light and soil temperature on club root disease[J]. Ann Appl Biol,1978,88:229-238.
[33]尹全,宋君,刘勇,等.土壤根肿病菌休眠孢子PCR快速检测方法的建立[J].西南农业学报,2010,23(2):390-392.
[34]杨家莺,孙道旺,杨明英,等.75%达克宁防治白莱根肿病产量损失测定及残留分析[J].西南农业学报,2002,17(2):189-191.
[35]杨佩文,尚慧,董丽英,等.大白菜根肿病化学防治技术[J].植物保护,2009,35(6):157-159.
[36]王贵斌,王新生,卢惠芝,等.氰霜唑预防大白菜根肿效果好[J].农药市场信息,2012,28:41.
[37] Hiroharu Murakami, Seiya Tsushima, Yuldko Kuroyanagi, et al.Reduction of resting spore density of Plasmodiophora brasicae and clubroot disease severity by lining[J]. Soil science and plant nutrition.2002,48(5):685-691.
[38]戴玉华,杨莲,李顺德,等.调节土壤p H防治白菜根肿病技术探索[J].中国植保导刊,2004,24(8):20-22.
[39]李壮,牛应泽,吴永成,等.根肿病的研究进展[J].西南农业学报,2013(4):1733-1737.
[40] Buczacki S T, Ockendon J G, Freeman G H. An analysis of some effects of light and soil temperature on clubroot disease[J]. Ann Appl Biol,1978(88):229-238.
[41] Buczacki T, Moxham S E. Structure of the resting spore wall of Plasmodiophora brassicae revealed by electron microscopy and chemical digestion[J]. Transactions of the British Mycological Society,1983,81(2):221-231
[42]郭向华.甘蓝根肿病菌的生物学特性及致病研究[D].重庆:西南农业大学,2001.
[43] Dixon G R. Variation in Plasmodiophora brassicae[J]. Annals of Applied Biology,1980,94(2):278-280
[44]马淑青,黄云,王铮一,等.油菜根肿病菌形态及生物学特性研究[J].四川农业大学学报,2006(2):161-165.
[45] Macfarlane I. Germination of resting spores of Plasmodiophora brassicae[J]. Transactions of the British Mycological Society,1970,55(1):97-112.
[46]李明远.十字花科根肿病的发生及防治[J].北京农业科学,1990,8(4):11-13.
[47]谢文瑞.十字花科蔬菜根瘤病菌之生态[A].作物绝对寄生真菌病害研讨会专刊[C].1992:145-146.
[48]吕理燊.十字花科蔬菜根肿病[J].云南农业大学学报,2002,17(2):134-136.