盐胁迫下野牛草相连分株根尖钙离子流变化
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摘要
克隆植物对异质生境具有不同的可塑性反应。本研究以克隆植物野牛草(Buchloe dactyloides)相连姊妹分株为材料,利用非损伤微测技术,通过测定正常条件和NaCl胁迫下,野牛草克隆片段姊妹株根尖Ca~(2+)净流速量的变化规律,研究克隆植物根尖Ca~(2+)流对NaCl胁迫的响应,及生理整合对相连克隆构件根尖Ca~(2+)流的调控作用。结果表明,NaCl胁迫促进了野牛草姊株和妹株根尖Ca~(2+)外流,且Ca~(2+)外流主要通过位于质膜上的电压依赖型钙泵完成。野牛草克隆分株间存在NaCl胁迫信号的整合,调控了NaCl胁迫下野牛草分株根尖Ca~(2+)流的变化。Ca~(2+)参与克隆分株对异质营养的感知和应答,调控了克隆分株间的营养生理整合。
Clonal plants exhibit different phenotypic responses to habitat heterogeneity.Ca~(2+) net flux response of root tips to NaCl stress and resultant modification of physiological integration were studied by measuring Ca~(2+) net fluxes of older and younger ramet root tips of Buchloe dactyloides under different NaCl stress treatments using non-invasive micro-test technique.The results showed that NaCl stress increases outflow of Ca~(2+) via voltage-dependent calcium pumps in the plasma membrane.Ca~(2+) net fluxes of root tips were modified by physiological integration of NaCl stress signals between older and younger ramets.Physiological integration was modified by the Ca~(2+) signal of ramets in response to nutrient heterogeneity.
Clonal plants exhibit different phenotypic responses to habitat heterogeneity.Ca~(2+) net flux response of root tips to NaCl stress and resultant modification of physiological integration were studied by measuring Ca~(2+) net fluxes of older and younger ramet root tips of Buchloe dactyloides under different NaCl stress treatments using non-invasive micro-test technique.The results showed that NaCl stress increases outflow of Ca~(2+) via voltage-dependent calcium pumps in the plasma membrane.Ca~(2+) net fluxes of root tips were modified by physiological integration of NaCl stress signals between older and younger ramets.Physiological integration was modified by the Ca~(2+) signal of ramets in response to nutrient heterogeneity.
引文
[1]VAN GROENENDAEL J,DE KROON H.Clonal Growth in Plants:Regulation and Function.SPB Academic.1990,12:56-61.
[2]LECHOWICZ M J,BELL G.The ecology and genetics of fitness in forest plants.II.Microspatial heterogeneity of the edaphic environment.Journal of Ecology,1991,79(3):687-696.
[3]JACKSON R B,CALDWELL M M.Geostatistical patterns of soil heterogeneity around individual perennial plants.Journal of Ecology,1993,81(4):683-692.
[4]CAIN M L,SUBLER S,EVANS J P.Sampling spatial and temporal variation in soil nitrogen.Oecologia,1999,118(4):397-404.
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[6]董鸣.异质生境中的植物克隆生长:风险分滩.植物生态学报,1996a,20(6):543-548.DONG M.Plant clonal growth in heterogeneous habitats:Risks-spreading.Acta Phytoecologica Sinica,1996a,20(6):543-548.
[7]董鸣.资源异质性环境的植物克隆生长:觅食行为.植物学报,1996b,38(10):828-835.DONG M.Clonal growth in plants in relation to resource heterogeneity:Foraging behavior.Acta Botanica Sinica,1996b,38(10):828-835.
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[10]SAITOH T,SEIWA K,NISHIWAKI A.Importance of physiological integration of dwarf bamboo to persistence in forest under storey:A field experiment.Journal of Ecology,2002,90:78-85.
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[12]WIJESINGHE D K,HANDEL S T.Advantages of clonal growth in heterogeneous habitats:An experiment with Potentilla simplex.Journal of Ecology,1994,82:495-502.
[13]ALPERT P.Nitrogen sharing in natural clonal fragments of Fragaria chiloensis.Ecology,1996,84(3):395-406.
[14]ZHANG L L,HE W M.Consequences of ramets helping ramets:No damage and increased nutrient use efficiency in nurse ramets of Glechoma hederacea.Flora,2009,204(3):182-188.
[15]STUEFER J F.Potential and limitations of current concepts regarding the response of clonal plants to environmental heterogeneity.Vegetatio,1996,127:55-70.
[16]HUBER H,FIJAN A,During H.A comparative study of spacer plasticity in erect and stoloniferous herbs.Oikos,1998,81(3):576-586.
[17]DE KROON H,HUBER H,STUEFER J F,VAN GROENENDACL J M.A modular concept of phenotypic plasticity in plants.New Phytologist,2005,166:73-82.
[18]MARSHALL C,SAGAR G R.The distribution of assimilates in Lolium multiflorum Lam.following differential defoliation.Annual of Botany,1968,32:715-719.
[19]MARSHALL C,SAGAR G R.The interdependence of tillers in Lolium multiflorum Lam.:A quantitative assessment.Journal of Experimental Botany,1968b,19:785-794.
[20]QIAN Y Q,LI D Y,HAN L,SUN Z Y.Inter-ramet physiological integration detected for lipid peroxidation and antioxidant enzymes activities in buffalograss(Buchloe dactyloides(Nutt.)Engelm.)under water stress.Korean Journal of Turfgrass Science,2009,23(2):331-344.
[21]QIAN Y Q,LI D Y,HAN L,SUN Z Y.Inter-ramet photosynthate translocation in buffalograss under differential water stress.Journal of the American Society for Horticultural Science,2010,135(4):308-314.
[22]潘瑞炽.植物生理学.北京:高等教育出版社.2004.PAN R Z.Plant Physiology.Beijing:Advanced Education Publishing House.2004.
[23]YANG T B,POOVAIAH B W.Calcium/calmodulin mediated signal network in plants.Plant Science,2003,8(10):505-512.
[24]HETHERINGTON A M,BROWNLEE C.The generation of calcium signals in plants:A review.Plant Biology,2004,55:401-427.
[25]KUHTREIBER W,JAFFE L.Detection of extracellular calcium gradients with a calcium-specific vibrating electrode.Journal of Cell Biology,1990,110(5):1565-1573.
[26]印莉萍,上官宇,许越.非损伤性扫描离子选择电极技术及其在高等植物研究中的应用.自然科学进展,2006,16(3):262-266.YIN L P,SHANGGUAN Y,XU Y.Non-invasive scanning ion selective electrode technology and its application in higher plant research.Advances in Nature Sciences,2006,16(3):262-266.
[27]丁亚男,许越.非损伤微测技术及其在生物医学研究中的应用.物理,2007,36(7):548-558.DING Y N,XU Y.Non-invasive micro-test technology and its applications in biology and medicine.Physics,2007,36(7):548-558.
[28]DODD A N,KUDLA J,SANDERS D.The language of calcium signaling.Annual Review of Plant Biology,2010,61(1):593-620.
[29]BIRCH C P D,HUTCHINGS M J.Exploitation of patchily distributed soil resources by the clonal herb Glechoma hederacea.Journal of Ecology,1994,82(3):653-664.
[2]LECHOWICZ M J,BELL G.The ecology and genetics of fitness in forest plants.II.Microspatial heterogeneity of the edaphic environment.Journal of Ecology,1991,79(3):687-696.
[3]JACKSON R B,CALDWELL M M.Geostatistical patterns of soil heterogeneity around individual perennial plants.Journal of Ecology,1993,81(4):683-692.
[4]CAIN M L,SUBLER S,EVANS J P.Sampling spatial and temporal variation in soil nitrogen.Oecologia,1999,118(4):397-404.
[5]HUTCHINGS M J,DE KROON H.Foraging in plants:The role of morphological plasticity in resource acquisition.Advances in Ecology Research,1994,25:159-238.
[6]董鸣.异质生境中的植物克隆生长:风险分滩.植物生态学报,1996a,20(6):543-548.DONG M.Plant clonal growth in heterogeneous habitats:Risks-spreading.Acta Phytoecologica Sinica,1996a,20(6):543-548.
[7]董鸣.资源异质性环境的植物克隆生长:觅食行为.植物学报,1996b,38(10):828-835.DONG M.Clonal growth in plants in relation to resource heterogeneity:Foraging behavior.Acta Botanica Sinica,1996b,38(10):828-835.
[8]PIGLIUCCI M.Evolution of phenotypic plasticity:Where are we going now.Trends in Ecology and Evolution,2005,20:486.
[9]SLADE A J,HUTCHINGS M J.An analysis of the costs and benefits of physiological integration between ramets in the clonal perennial herb Glechoma hederacea.Oecologia,1987,73:425-431.
[10]SAITOH T,SEIWA K,NISHIWAKI A.Importance of physiological integration of dwarf bamboo to persistence in forest under storey:A field experiment.Journal of Ecology,2002,90:78-85.
[11]SCHMID B.Some ecological and evolutionary consequences of modular organization and clonal growth in plants.Evolutionary Trends in Plants,1990,4(1):25-34.
[12]WIJESINGHE D K,HANDEL S T.Advantages of clonal growth in heterogeneous habitats:An experiment with Potentilla simplex.Journal of Ecology,1994,82:495-502.
[13]ALPERT P.Nitrogen sharing in natural clonal fragments of Fragaria chiloensis.Ecology,1996,84(3):395-406.
[14]ZHANG L L,HE W M.Consequences of ramets helping ramets:No damage and increased nutrient use efficiency in nurse ramets of Glechoma hederacea.Flora,2009,204(3):182-188.
[15]STUEFER J F.Potential and limitations of current concepts regarding the response of clonal plants to environmental heterogeneity.Vegetatio,1996,127:55-70.
[16]HUBER H,FIJAN A,During H.A comparative study of spacer plasticity in erect and stoloniferous herbs.Oikos,1998,81(3):576-586.
[17]DE KROON H,HUBER H,STUEFER J F,VAN GROENENDACL J M.A modular concept of phenotypic plasticity in plants.New Phytologist,2005,166:73-82.
[18]MARSHALL C,SAGAR G R.The distribution of assimilates in Lolium multiflorum Lam.following differential defoliation.Annual of Botany,1968,32:715-719.
[19]MARSHALL C,SAGAR G R.The interdependence of tillers in Lolium multiflorum Lam.:A quantitative assessment.Journal of Experimental Botany,1968b,19:785-794.
[20]QIAN Y Q,LI D Y,HAN L,SUN Z Y.Inter-ramet physiological integration detected for lipid peroxidation and antioxidant enzymes activities in buffalograss(Buchloe dactyloides(Nutt.)Engelm.)under water stress.Korean Journal of Turfgrass Science,2009,23(2):331-344.
[21]QIAN Y Q,LI D Y,HAN L,SUN Z Y.Inter-ramet photosynthate translocation in buffalograss under differential water stress.Journal of the American Society for Horticultural Science,2010,135(4):308-314.
[22]潘瑞炽.植物生理学.北京:高等教育出版社.2004.PAN R Z.Plant Physiology.Beijing:Advanced Education Publishing House.2004.
[23]YANG T B,POOVAIAH B W.Calcium/calmodulin mediated signal network in plants.Plant Science,2003,8(10):505-512.
[24]HETHERINGTON A M,BROWNLEE C.The generation of calcium signals in plants:A review.Plant Biology,2004,55:401-427.
[25]KUHTREIBER W,JAFFE L.Detection of extracellular calcium gradients with a calcium-specific vibrating electrode.Journal of Cell Biology,1990,110(5):1565-1573.
[26]印莉萍,上官宇,许越.非损伤性扫描离子选择电极技术及其在高等植物研究中的应用.自然科学进展,2006,16(3):262-266.YIN L P,SHANGGUAN Y,XU Y.Non-invasive scanning ion selective electrode technology and its application in higher plant research.Advances in Nature Sciences,2006,16(3):262-266.
[27]丁亚男,许越.非损伤微测技术及其在生物医学研究中的应用.物理,2007,36(7):548-558.DING Y N,XU Y.Non-invasive micro-test technology and its applications in biology and medicine.Physics,2007,36(7):548-558.
[28]DODD A N,KUDLA J,SANDERS D.The language of calcium signaling.Annual Review of Plant Biology,2010,61(1):593-620.
[29]BIRCH C P D,HUTCHINGS M J.Exploitation of patchily distributed soil resources by the clonal herb Glechoma hederacea.Journal of Ecology,1994,82(3):653-664.