三倍体古老月季在月季育种中的应用及其子代倍性分析
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摘要
为了探究三倍体中国古老月季的育种价值,本研究以4个三倍体中国古老月季为材料,通过杂交结实情况进行育性研究,并利用流式细胞术结合染色体压片对后代进行倍性检测来获得三倍体可育配子信息。结果显示:供试三倍体材料雌性不育,部分雄性可育,‘春水绿波’、‘羽士妆’可做父本且可产生大量后代;‘春水绿波’、‘羽士妆’做父本的4x♀×3x♂杂交组合中的后代为全部为四倍体,其为后代提供了2x配子;杂交子代可获得结实,可作为母本与三倍体回交。本研究表明‘春水绿波’、‘羽士妆’既可作为亲本也可作为中间材料将中国古老月季优秀基因传递到现代月季基因库中,并为后续三倍性育种研究提供理论基础。
In order to explore triploid old roses' value in hybridization, fertility of 4 triploid roses was estimated based on hybridization, and progenies of triploids were tested and analyzed by flow cytometry and chromosome compression method to obtain the information of triploid viable gametes. The results showed that 4 triploid was female-sterile but partly male-fertile, for Rosa 'Yushizhuang' and Rosa 'Chunshuilvbo' can produce many progenies;progenies of 4 x ♀ ×3 x ♂ combination which Rosa 'Yushizhuang' or Rosa 'Chunshuilvbo' as male parent are all tetraploid with offering 2 x pollen; obtained F1 are fertile and can backcross with triploids as female parent. This study proved Rosa 'Yushizhuang' and Rosa 'Chunshuilvbo' can not only be used as parents but also as intermediate materials to pass excellent genes of chinese old rose into gene pool of modern rose sand provided theoretical basis for future triploidy breeding.
In order to explore triploid old roses' value in hybridization, fertility of 4 triploid roses was estimated based on hybridization, and progenies of triploids were tested and analyzed by flow cytometry and chromosome compression method to obtain the information of triploid viable gametes. The results showed that 4 triploid was female-sterile but partly male-fertile, for Rosa 'Yushizhuang' and Rosa 'Chunshuilvbo' can produce many progenies;progenies of 4 x ♀ ×3 x ♂ combination which Rosa 'Yushizhuang' or Rosa 'Chunshuilvbo' as male parent are all tetraploid with offering 2 x pollen; obtained F1 are fertile and can backcross with triploids as female parent. This study proved Rosa 'Yushizhuang' and Rosa 'Chunshuilvbo' can not only be used as parents but also as intermediate materials to pass excellent genes of chinese old rose into gene pool of modern rose sand provided theoretical basis for future triploidy breeding.
引文
Adiwila ga K.D.,and Brown C.R.,1991,Use of 2n pollen-producing triploid hybrids to introduce tetraploid Mexican wild species germ plasm to cultivated tetraploid potato gene pool,Theor.Appl.Genet.,81(5):645-652
Bakry F.,and Horry J.P.,1992,Tetraploid hybrids from interploid3x/2x crosses in cooking bananas,Fruits,47(6):641-647
Barba-Gonzalez R.,Silfhout A.A.V.,Visser R.G.F.,Ramanna M.S.,and Tuyl J.M.V.,2006,Progenies of allotriploids of Oriental×Asiatic lilies(Lilium)examined by GISH analysis,E-uphytica,151(2):243-250
Cao Q.Z.,Gao X.,and Jia G.X.,2016,Selection of distant hybridization parents for triploid LA hybrid lilies,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),38(2):96-104(曹钦政,高雪,贾桂霞,2016,三倍体LA百合远缘杂交亲本的筛选,北京林业大学学报,38(2):96-104)
Castro S.,Loureiro J.,Rodriguez E.,Silveira P.,Navarro L.,and Santos C.,2007,Evaluation of polysomaty and estimatio n of genome size in Polygala vayredae and Plcalcarea using flow cytometry,Plant Science.,172(6):1131-1137
Farnham M.W.,Caniglia E.J.,and Thomas C.E.,1998,Efficient ploidy determination of anther-derived broccoli,HortScience,32(2):323-327
Ge B.B.,2016,Preliminary study on male gametophyte fertility of Rosa multiflora Thunb.and cultivar'Chunshui Lǜbo',Thesis for M.S.,Henan Agriculture University,Supervisor:Wu R.H.,pp.32(葛蓓蓓,2016,粉团蔷薇及‘春水绿波’月季雄配子可育性初步研究,硕士学位论文,河南农业大学,导师:武荣花,pp.32)
Hard M.L.,Verhagen S.,Winton L.,and Einspahr D.W.,1976,Tetraploid aspen production using unreduced pollen from triploid males,For.Sci.,22(3):329-330
Haunold A.,1970,Fertility studies and cytological analysis of the progeny of a triploid×diploid cross in hop,Humulus lupulus L.,Genome,12(3):582-588
He D.,Xie M.J.,LüB.Y.,Wang Z.,Liu Y.P.,and He S.L.,2017,Analysis of pollination affinity performance and its physiological mechanism,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University(Natural Science Edition)),45(10):129-136(贺丹,解梦珺,吕博雅,王政,刘艺平,何松林,2017,牡丹与芍药的授粉亲和性表现及其生理机制分析,西北农林科技大学学报(自然科学版),45(10):129-136)
Herklotz V.,Kovarík A.,LunerováJ.,Lippitsch S.,Groth M.,and Ritz C.M.,2018,The fate of ribosomal RNA genes in spontaneous polyploid dogrose hybrids(Rosa L.sect.Caninae(DC.)Ser.)exhibiting non-symmetrical meiosis,Plant J.,94(1):77-90
Huylenbroeck J.V.,Leus L.,and Bockstaele E.V.,2005,Interploidy crosses in roses;use of triploids,Acta Horticulturae,690:109-112
Jian H.Y.,Zhang H.,Wang Q.G.,Tang K.X.,Li S.F.,Qiu X.Q.,Yan H.J.,and Zhang T.,2010,Karylogical study of Chinese old garden roses,Yuanyi Xuebao(Acta Horticulturae Sinica),37(1):83-88(蹇洪英,张颢,王其刚,唐开学,李树发,邱显钦,晏慧君,张婷,2010,中国古老月季品种的核型研究,园艺学报,37(1):83-88)
Kang X.Y.,and Mao J.F.,2001,Gamete fertility and morphological variations in offsprings of triploid clones Populus tomentosa,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),23(4):20-23(康向阳,毛建丰,2001,三倍体毛白杨配子育性及其子代形态变异研究,北京林业大学学报,23(4):20-23)
Kikuchi S.,Iwasuna M.,Kobori A.,Tsutaki Y.,Yoshida A.,Murota Y.,Nishino E.,Sassa H.,and Koba T.,2014,Seed formation in triploid loquat(Eriobotrya japonica)through cross-hybridization with pollen of diploid cultivars,Breed Sci.,64(2):176-182
Liang W.J.,Xie K.D.,Xie Z.Z.,Xu Q.,Yi H.L.,and Guo W.W.,2015,Exploitation of polyploids from open-pollinated triploid grapefruit progenies and their genetic identification by SSR molecular markers,Guoshu Xuebao(Journal of Fruit Science),32(1):13-18(梁武军,解凯东,谢宗周,徐强,伊华林,郭文武,2015,三倍体葡萄柚实生后代多倍体的发掘与SSR遗传鉴定,果树学报,32(1):13-18)
Lim K.Y.,Werlemark G.,Matyasek R.,Bringloe J.B.,Sieber V.,and Mokadem H.E.,Meynet J.,Hemming J.,Leitch A.R.,and Roberts A.V.,2005,Evolutionary implications of permanent odd polyploidy in the stable sexual,pentaploid of Rosa canina L.,Heredity,94(5):501-506
Liu Q.M.,Wang Y.W.,and Wang X.S.,2012,Detection of chromosome ploidy level in Dactylis glomerata and Lolium multiflorum by flow cytometry,Caoye Kexue(Pratacultural Science),29(3):403-410(柳青慕,王赟文,王小山,2012,流式细胞仪快速检测鸭茅与多花黑麦草染色体倍性的研究,草业科学,29(3):403-410)
Luo L.,Zhang Q.X.,Bai J.R.,Pan H.T.,Li H.,and Wang Q.,2009,Karyotype analysis of sixteen Chinese traditional rose cultivars,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),31(5):90-95(罗乐,张启翔,白锦荣,潘会堂,李卉,王琼,2009,16个中国传统月季品种的核型分析,北京林业大学学报,31(5):90-95)
Lysák M.A.,Dole觩elováM.,Horry J.P.,Swennen R.,and Dole觩el J.,1999,Flow cytometric analysis of nuclear DNA content in Musa,Theor.Appl.Genet.,98(8):1344-1150
Mallón R.,Rodríguez-Oubi觡a J.,and González M.L.,2010,In vitro propagation of the endangered plant Centaurea ultreiae:assessment of genetic stability by cytological studies,flow cytometry and RAPD analysis,Plant Cell Tissue and Organ Culture,101(1):31-39
Peckert T.,and Jun J.C.,2006,Mating interactions between coexisting diploid,triploid and tetraploid cytotypes of Hieracium echioides(Asteraceae),Folia Geobotanica,41(3):323-334
Shi C.H.,Xu LL.,Wang X.Q.,and Luo J.,2014,Identification of kiwifruit polidy using flow cytometry,Zhiwu Yanjiu(Bulletin of Botanical Research),34(6):845-849(施春晖,徐兰兰,王晓庆,骆军,2014,流式细胞仪鉴定猕猴桃倍性技术研究,植物研究,34(6):845-849)
Sha Y.H.,Liu L.,Wang K.,Gong Y.Q.,Le C.T.,Zhang C.P.,and Liu L.W.,2009,Karyotype analysis of three types of radish germplasms with different tap-root skin colors,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1141-1145(沙玉辉,刘莉,王康,龚义勤,Le C.T.,张翠萍,柳李旺,2009,3种不同皮色萝卜种质染色体核型分析,基因组学与应用生物学,34(6):1141-1145)
Sun P.,Dai L.,Xu Z.H.,Sun F.,Yuan C.Q.,Hu R.Y.,Sun Y.H.,and Li Y.,2015,Distribution of Ca2+in the stigma and style of Robinia pseudoacacia during pollen germination and pollen tube elongation,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),37(11):59-68(孙鹏,戴丽,徐兆翮,孙非,袁存权,胡瑞阳,孙宇涵,李云,2015,刺槐花粉萌发和花粉管伸长生长过程中柱头与花柱内钙离子的分布特征,北京林业大学学报,37(11):59-68)
Wu R.H.,Ge B.B.,Wang M.L.,Zhou Y.,and Feng H.,2016,Estimation of genome size of eighteen Chinese old garden roses by flow eytometry,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),38(6):94-100(武荣花,葛蓓蓓,王茂良,周燕,冯慧,2016,应用流式细胞术测定18个中国古老月季基因组大小,北京林业大学学报,38(6):94-100)
Yuan G.L.,Xu P.,Gong H.X.,Zhou F.H.,and Zhou S.J.,2013,Lily(Lilium)introgression breeding using triploid cultivars as maternal parents,Nongye Shengwu Jishu Xuebao(Jour nal of Agricultural Biotechnology),21(11):1321-1327(袁国良,徐萍,宫红霞,周法华,周树军,2013,用三倍体作母本实现百合种质渗入育种,农业生物技术学报,21(11):1321-1327)
Zhang J.,Wu X.J.,Wang X.D.,Zhou K.D.,and Peng H.,2002,Cytological study on a special autotriploid rice SAR-3,Zuowu Xuebao(Acta Agronomica Sinica),28(5):704-708(张静,吴先军,汪旭东,周开达,彭海,2002,特异同源三倍体水稻材料SAR-3细胞学研究,作物学报,28(5):704-708)
Zhang X.,Cao Q.,Zhou P.,and Jia G.,2017,Meiotic chromosome behavior of the male-fertile allotriploid lily cultivar'Cocossa',Plant Cell Reports.,36(10):1641-1653
Zhou S.J.,Zhou G.X.,and Li K.H.,2011,Euploid endosperm of triploid×diploid/tetraploide crosses results in aneuploid embryo survival in Lilium,Hort.Science.,46(4):558-562
Bakry F.,and Horry J.P.,1992,Tetraploid hybrids from interploid3x/2x crosses in cooking bananas,Fruits,47(6):641-647
Barba-Gonzalez R.,Silfhout A.A.V.,Visser R.G.F.,Ramanna M.S.,and Tuyl J.M.V.,2006,Progenies of allotriploids of Oriental×Asiatic lilies(Lilium)examined by GISH analysis,E-uphytica,151(2):243-250
Cao Q.Z.,Gao X.,and Jia G.X.,2016,Selection of distant hybridization parents for triploid LA hybrid lilies,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),38(2):96-104(曹钦政,高雪,贾桂霞,2016,三倍体LA百合远缘杂交亲本的筛选,北京林业大学学报,38(2):96-104)
Castro S.,Loureiro J.,Rodriguez E.,Silveira P.,Navarro L.,and Santos C.,2007,Evaluation of polysomaty and estimatio n of genome size in Polygala vayredae and Plcalcarea using flow cytometry,Plant Science.,172(6):1131-1137
Farnham M.W.,Caniglia E.J.,and Thomas C.E.,1998,Efficient ploidy determination of anther-derived broccoli,HortScience,32(2):323-327
Ge B.B.,2016,Preliminary study on male gametophyte fertility of Rosa multiflora Thunb.and cultivar'Chunshui Lǜbo',Thesis for M.S.,Henan Agriculture University,Supervisor:Wu R.H.,pp.32(葛蓓蓓,2016,粉团蔷薇及‘春水绿波’月季雄配子可育性初步研究,硕士学位论文,河南农业大学,导师:武荣花,pp.32)
Hard M.L.,Verhagen S.,Winton L.,and Einspahr D.W.,1976,Tetraploid aspen production using unreduced pollen from triploid males,For.Sci.,22(3):329-330
Haunold A.,1970,Fertility studies and cytological analysis of the progeny of a triploid×diploid cross in hop,Humulus lupulus L.,Genome,12(3):582-588
He D.,Xie M.J.,LüB.Y.,Wang Z.,Liu Y.P.,and He S.L.,2017,Analysis of pollination affinity performance and its physiological mechanism,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University(Natural Science Edition)),45(10):129-136(贺丹,解梦珺,吕博雅,王政,刘艺平,何松林,2017,牡丹与芍药的授粉亲和性表现及其生理机制分析,西北农林科技大学学报(自然科学版),45(10):129-136)
Herklotz V.,Kovarík A.,LunerováJ.,Lippitsch S.,Groth M.,and Ritz C.M.,2018,The fate of ribosomal RNA genes in spontaneous polyploid dogrose hybrids(Rosa L.sect.Caninae(DC.)Ser.)exhibiting non-symmetrical meiosis,Plant J.,94(1):77-90
Huylenbroeck J.V.,Leus L.,and Bockstaele E.V.,2005,Interploidy crosses in roses;use of triploids,Acta Horticulturae,690:109-112
Jian H.Y.,Zhang H.,Wang Q.G.,Tang K.X.,Li S.F.,Qiu X.Q.,Yan H.J.,and Zhang T.,2010,Karylogical study of Chinese old garden roses,Yuanyi Xuebao(Acta Horticulturae Sinica),37(1):83-88(蹇洪英,张颢,王其刚,唐开学,李树发,邱显钦,晏慧君,张婷,2010,中国古老月季品种的核型研究,园艺学报,37(1):83-88)
Kang X.Y.,and Mao J.F.,2001,Gamete fertility and morphological variations in offsprings of triploid clones Populus tomentosa,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),23(4):20-23(康向阳,毛建丰,2001,三倍体毛白杨配子育性及其子代形态变异研究,北京林业大学学报,23(4):20-23)
Kikuchi S.,Iwasuna M.,Kobori A.,Tsutaki Y.,Yoshida A.,Murota Y.,Nishino E.,Sassa H.,and Koba T.,2014,Seed formation in triploid loquat(Eriobotrya japonica)through cross-hybridization with pollen of diploid cultivars,Breed Sci.,64(2):176-182
Liang W.J.,Xie K.D.,Xie Z.Z.,Xu Q.,Yi H.L.,and Guo W.W.,2015,Exploitation of polyploids from open-pollinated triploid grapefruit progenies and their genetic identification by SSR molecular markers,Guoshu Xuebao(Journal of Fruit Science),32(1):13-18(梁武军,解凯东,谢宗周,徐强,伊华林,郭文武,2015,三倍体葡萄柚实生后代多倍体的发掘与SSR遗传鉴定,果树学报,32(1):13-18)
Lim K.Y.,Werlemark G.,Matyasek R.,Bringloe J.B.,Sieber V.,and Mokadem H.E.,Meynet J.,Hemming J.,Leitch A.R.,and Roberts A.V.,2005,Evolutionary implications of permanent odd polyploidy in the stable sexual,pentaploid of Rosa canina L.,Heredity,94(5):501-506
Liu Q.M.,Wang Y.W.,and Wang X.S.,2012,Detection of chromosome ploidy level in Dactylis glomerata and Lolium multiflorum by flow cytometry,Caoye Kexue(Pratacultural Science),29(3):403-410(柳青慕,王赟文,王小山,2012,流式细胞仪快速检测鸭茅与多花黑麦草染色体倍性的研究,草业科学,29(3):403-410)
Luo L.,Zhang Q.X.,Bai J.R.,Pan H.T.,Li H.,and Wang Q.,2009,Karyotype analysis of sixteen Chinese traditional rose cultivars,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),31(5):90-95(罗乐,张启翔,白锦荣,潘会堂,李卉,王琼,2009,16个中国传统月季品种的核型分析,北京林业大学学报,31(5):90-95)
Lysák M.A.,Dole觩elováM.,Horry J.P.,Swennen R.,and Dole觩el J.,1999,Flow cytometric analysis of nuclear DNA content in Musa,Theor.Appl.Genet.,98(8):1344-1150
Mallón R.,Rodríguez-Oubi觡a J.,and González M.L.,2010,In vitro propagation of the endangered plant Centaurea ultreiae:assessment of genetic stability by cytological studies,flow cytometry and RAPD analysis,Plant Cell Tissue and Organ Culture,101(1):31-39
Peckert T.,and Jun J.C.,2006,Mating interactions between coexisting diploid,triploid and tetraploid cytotypes of Hieracium echioides(Asteraceae),Folia Geobotanica,41(3):323-334
Shi C.H.,Xu LL.,Wang X.Q.,and Luo J.,2014,Identification of kiwifruit polidy using flow cytometry,Zhiwu Yanjiu(Bulletin of Botanical Research),34(6):845-849(施春晖,徐兰兰,王晓庆,骆军,2014,流式细胞仪鉴定猕猴桃倍性技术研究,植物研究,34(6):845-849)
Sha Y.H.,Liu L.,Wang K.,Gong Y.Q.,Le C.T.,Zhang C.P.,and Liu L.W.,2009,Karyotype analysis of three types of radish germplasms with different tap-root skin colors,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1141-1145(沙玉辉,刘莉,王康,龚义勤,Le C.T.,张翠萍,柳李旺,2009,3种不同皮色萝卜种质染色体核型分析,基因组学与应用生物学,34(6):1141-1145)
Sun P.,Dai L.,Xu Z.H.,Sun F.,Yuan C.Q.,Hu R.Y.,Sun Y.H.,and Li Y.,2015,Distribution of Ca2+in the stigma and style of Robinia pseudoacacia during pollen germination and pollen tube elongation,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),37(11):59-68(孙鹏,戴丽,徐兆翮,孙非,袁存权,胡瑞阳,孙宇涵,李云,2015,刺槐花粉萌发和花粉管伸长生长过程中柱头与花柱内钙离子的分布特征,北京林业大学学报,37(11):59-68)
Wu R.H.,Ge B.B.,Wang M.L.,Zhou Y.,and Feng H.,2016,Estimation of genome size of eighteen Chinese old garden roses by flow eytometry,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),38(6):94-100(武荣花,葛蓓蓓,王茂良,周燕,冯慧,2016,应用流式细胞术测定18个中国古老月季基因组大小,北京林业大学学报,38(6):94-100)
Yuan G.L.,Xu P.,Gong H.X.,Zhou F.H.,and Zhou S.J.,2013,Lily(Lilium)introgression breeding using triploid cultivars as maternal parents,Nongye Shengwu Jishu Xuebao(Jour nal of Agricultural Biotechnology),21(11):1321-1327(袁国良,徐萍,宫红霞,周法华,周树军,2013,用三倍体作母本实现百合种质渗入育种,农业生物技术学报,21(11):1321-1327)
Zhang J.,Wu X.J.,Wang X.D.,Zhou K.D.,and Peng H.,2002,Cytological study on a special autotriploid rice SAR-3,Zuowu Xuebao(Acta Agronomica Sinica),28(5):704-708(张静,吴先军,汪旭东,周开达,彭海,2002,特异同源三倍体水稻材料SAR-3细胞学研究,作物学报,28(5):704-708)
Zhang X.,Cao Q.,Zhou P.,and Jia G.,2017,Meiotic chromosome behavior of the male-fertile allotriploid lily cultivar'Cocossa',Plant Cell Reports.,36(10):1641-1653
Zhou S.J.,Zhou G.X.,and Li K.H.,2011,Euploid endosperm of triploid×diploid/tetraploide crosses results in aneuploid embryo survival in Lilium,Hort.Science.,46(4):558-562