高效脱氮菌的分离、鉴定及功能分析
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摘要
利用富集培养法从高氨氮废水中分离得到1株高效氨氮降解菌,命名为LSH2。通过形态、生理生化特性和16S rRNA基因序列分析,初步鉴定为产碱杆菌。脱氮实验结果显示:当溶解氧值(Dissolved Oxygen,DO)为4.0～6.0 mg/L,培养24 h时,可使初始浓度为121 mg/L的氨氮下降到17 mg/L,氨氮去除率达86%。以组合载体为填料,挂膜后对污水进行处理,停留时间48 h,氨氮去除率可达87%。针对该菌的研究对建立脱氮体系有积极意义。
A high-efficiency bacterial strain named LSH2 was isolated from the high ammonia nitrogen wastewater by enrichment culture.Strain LSH2 was identified as Alcaligenes according to morphological characteristics,physiological and biochemical characteristics,and the analysis of partial 16 S rRNA gene sequence. The result showed that when the DO was at the range of 4.0-6.0 mg/L,the ammonia nitrogen can be reduced from 121 mg/L to 17 mg/L,the removal rate reached 86% under incubation time of 24 h.The wastewater was treated by this bacterial which were packed in film,when HRT was 48 h,the removal rate was up to 87%. The study on the bacteria has positive significance for the establishment of nitrogen removal system.
A high-efficiency bacterial strain named LSH2 was isolated from the high ammonia nitrogen wastewater by enrichment culture.Strain LSH2 was identified as Alcaligenes according to morphological characteristics,physiological and biochemical characteristics,and the analysis of partial 16 S rRNA gene sequence. The result showed that when the DO was at the range of 4.0-6.0 mg/L,the ammonia nitrogen can be reduced from 121 mg/L to 17 mg/L,the removal rate reached 86% under incubation time of 24 h.The wastewater was treated by this bacterial which were packed in film,when HRT was 48 h,the removal rate was up to 87%. The study on the bacteria has positive significance for the establishment of nitrogen removal system.
引文
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[2]PARVANOVA-MANCHEVA T,BESCHKOV V.Microbial denitrification by immobilized bacteria Pseudomonas denitrificans stimulated by constant electric field[J].Biochemical Engineering Journal,2009,44(2-3):208-213.
[3]ARTS P A M,ROBERTSON L A,KUENEN J G.Nitrification and denitrification by Thiosphaera pantotropha in aerobic chemostat cultures[J].FEMS Microbiology Ecology,1988,18(4):305-315.
[4]MEIBERG J B M,BRUINENBERG P M,HARDER W.Effect of Dissolved Oxygen Tension on the Metabolism of Methylated Amines in Hyphomicrobium X in the Absence and Presence of Nitrate:Evidence for 'Aerobic' Denitrification[J].Microbiology,1980,120(2):453-463.
[5]RANDALL C W,BAMARD J I.,STENSEL H D.Design and retrofit of wastewater treatment plants for biological nutrient removal[M].Bosa Roca:Taylor & Francis Inc,1998.
[6]王洁,蓝江林,刘波.一株异养硝化细菌的分离鉴定和脱氮特性研究[J].农业环境科学报,2013,32(4):805-810.
[7]翟茜,汪萍,李秀婷,等.活性污泥中好氧反硝化菌的富集筛选及鉴别[J].环境科学与技术,2007,30(1):11-13.
[8]魏巍.微污染水源扬水曝气强化原位生物脱氮特性与试验研究[D].西安:西安建筑科技大学,2011.
[9]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:北京科学出版社,2001:370-390.
[10]黄延林,白士远,张海涵,等.一株贫营养异养硝化-好氧反硝化细菌的分离鉴定及脱氮特性[J].环境工程学报,2015,9(12):5665-5671.
[11]国家环境保护部.水质氨氮的测定蒸馏-中和滴定法:HJ537-2009 [S].北京:中国环境出版社,2009.
[12]王兆阳,陈国耀,姜珂,等.一株耐冷兼性嗜碱好氧反硝化菌的分离鉴定及反硝化特性[J].环境科学,2014,35(6):2341-2348.