用mtDNA序列鉴定出上海海域须鲸科物种大村鲸
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摘要
测定了采自上海海域的一头死亡须鲸骨骼样本的线粒体DNA(mtDNA)细胞色素b基因(Cyt b)序列330 bp(登录号MK295815)、细胞色素C氧化酶Ⅰ基因(COⅠ)序列206 bp(登录号MK317953)和控制区(control region)序列231 bp(登录号MK317954)。通过GenBank中的BLAST分析,结果表明,样本的上述三部分序列与大村鲸(Balaenopteraomurai)对应序列的相似性均达到100%,基于最大似然法(ML)法构建的系统发育树与BLAST结果一致,故将标本鉴定为大村鲸,为上海海域首次记录。
The portions of the mitochondrial DNA(mtDNA) Cyt b gene(330 bp, GenBank accession:MK295815), cytochrome c oxidase subunit I(COⅠ)(206 bp, GenBank accession: MK317953) and mitochondrial DNA(mtDNA) control region(231 bp, GenBank accession: MK317954) of the skeleton sample from a dead baleen whale collected at the Shanghai seas were sequenced. The blasting result in Gen Bank shows that the three sequences have maximal similarity at 100% with those of Balaenoptera omurai(Table 1﹣3); and the phylogenetic trees based on maximum likelihood are consistent with the blasting result(Fig. 5﹣7). The result shows that the sample should be identified as B. omurai, and it is recorded for the first time from Shanghai Seas.
The portions of the mitochondrial DNA(mtDNA) Cyt b gene(330 bp, GenBank accession:MK295815), cytochrome c oxidase subunit I(COⅠ)(206 bp, GenBank accession: MK317953) and mitochondrial DNA(mtDNA) control region(231 bp, GenBank accession: MK317954) of the skeleton sample from a dead baleen whale collected at the Shanghai seas were sequenced. The blasting result in Gen Bank shows that the three sequences have maximal similarity at 100% with those of Balaenoptera omurai(Table 1﹣3); and the phylogenetic trees based on maximum likelihood are consistent with the blasting result(Fig. 5﹣7). The result shows that the sample should be identified as B. omurai, and it is recorded for the first time from Shanghai Seas.
引文
Baker C S,Cipriano F,Palumbi S R.1996.Molecular genetic identification of whale and dolphin products from commercial markets in Korea and Japan.Molecular Ecology,5(5):671-685.
Baker C S,Lento G M,Cipriano F,et al.2000.Scientific whaling:source of illegal products for market?Science,290(5497):1695-1696.
Baker C S,Palumbi S R.1994.Which whales are hunted?Amolecular genetic approach to monitoring whaling.Science,265(5178):1538-1540.
Cerchio S,Andrianantenaina B,Lindsay A,et al.2015.Omura's whales(Balaenoptera omurai)off northwest Madagascar:ecology,behaviour and conservation needs.Royal Society Open Science,2(10):150301.
Cypriano-Souza A L,Meirelles A C O,Carvalho V L,et al.2017.Rare or cryptic?The first report of an Omura's whale(Balaenoptera omurai)in the South Atlantic Ocean.Marine Mammal Science,33(1):80-95.
Dalebout M L,van Helden A,van Waerebeek K,et al.1998.Molecular genetic identification of southern hemisphere beaked whales(Cetacea:Ziphiidae).Molecular Ecology,7(6):687-694.
Darriba D,Taboada G,Doallo R,et al.2012.jModelTest 2:more models,new heuristics and parallel computing.Nature Methods,9(8):772-772.
Kim J H,Kim H W,Kim E M,et al.2018.First record of the Omura’s Whale(Balaenoptera omurai)in Korean Waters.Animal Systematics,Evolution and Diversity,34(3):162-167.
Kocher T D,Thomas W K,Meyer A,et al.1989.Dynamics of mitochondrial DNA evolution in animals:amplification and sequencing with conserved primers.Proceedings of the National Academy of Sciences of the United States of America,86(16):6196-6200.
Kumar S,Stecher G,Tamura K.2016.MEGA7:Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.Molecular Biology&Evolution,33(7):1870-1874.
Rosel P E,Wilcox L A.2014.Genetic evidence reveals a unique lineage of Bryde’s whales in the northern Gulf of Mexico.Endangered Species Research,25(1):19-34.
Wada S,Oishi M,Yamada T K.2003.A newly discovered species of living baleen whale.Nature,426(6964):278-281.
Xu M,Wang X,Miao X,et al.2017.A Stranding Record of Omura’s Whale(Balaenoptera omurai Yamada,2003)in the Taiwan Strait,China.Aquatic Mammals,43(3):289-298.
Yoshida H,Kato H.1999.Phylogenetic relationships of Bryde’s whales in the western North Pacific and adjacent waters inferred from mitochondrial DNA sequences.Marine Mammal Science,15(4):1269-1286.
马牧,祝茜,李响,等.2007.利用Cyt b基因由未知鲸骨鉴定出大村鲸.兽类学报,27(3):288-292.
王火根,范忠勇,沈宏,等.2006.中国大陆海域须鲸科的新纪录.水产科学,25(2):85-87.
王丕烈.2012.中国鲸类.北京:化学工业出版社,76-81.
杨光,刘海,周开亚,等.2002.用mt DNA序列鉴定一头小布氏鲸标本.动物学杂志,37(4):35-38.
朱小静,何鑫,向余劲攻,等.2018.用mtDNA控制区序列鉴定长须鲸标本.动物学杂志,53(2):234-242.
Baker C S,Lento G M,Cipriano F,et al.2000.Scientific whaling:source of illegal products for market?Science,290(5497):1695-1696.
Baker C S,Palumbi S R.1994.Which whales are hunted?Amolecular genetic approach to monitoring whaling.Science,265(5178):1538-1540.
Cerchio S,Andrianantenaina B,Lindsay A,et al.2015.Omura's whales(Balaenoptera omurai)off northwest Madagascar:ecology,behaviour and conservation needs.Royal Society Open Science,2(10):150301.
Cypriano-Souza A L,Meirelles A C O,Carvalho V L,et al.2017.Rare or cryptic?The first report of an Omura's whale(Balaenoptera omurai)in the South Atlantic Ocean.Marine Mammal Science,33(1):80-95.
Dalebout M L,van Helden A,van Waerebeek K,et al.1998.Molecular genetic identification of southern hemisphere beaked whales(Cetacea:Ziphiidae).Molecular Ecology,7(6):687-694.
Darriba D,Taboada G,Doallo R,et al.2012.jModelTest 2:more models,new heuristics and parallel computing.Nature Methods,9(8):772-772.
Kim J H,Kim H W,Kim E M,et al.2018.First record of the Omura’s Whale(Balaenoptera omurai)in Korean Waters.Animal Systematics,Evolution and Diversity,34(3):162-167.
Kocher T D,Thomas W K,Meyer A,et al.1989.Dynamics of mitochondrial DNA evolution in animals:amplification and sequencing with conserved primers.Proceedings of the National Academy of Sciences of the United States of America,86(16):6196-6200.
Kumar S,Stecher G,Tamura K.2016.MEGA7:Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.Molecular Biology&Evolution,33(7):1870-1874.
Rosel P E,Wilcox L A.2014.Genetic evidence reveals a unique lineage of Bryde’s whales in the northern Gulf of Mexico.Endangered Species Research,25(1):19-34.
Wada S,Oishi M,Yamada T K.2003.A newly discovered species of living baleen whale.Nature,426(6964):278-281.
Xu M,Wang X,Miao X,et al.2017.A Stranding Record of Omura’s Whale(Balaenoptera omurai Yamada,2003)in the Taiwan Strait,China.Aquatic Mammals,43(3):289-298.
Yoshida H,Kato H.1999.Phylogenetic relationships of Bryde’s whales in the western North Pacific and adjacent waters inferred from mitochondrial DNA sequences.Marine Mammal Science,15(4):1269-1286.
马牧,祝茜,李响,等.2007.利用Cyt b基因由未知鲸骨鉴定出大村鲸.兽类学报,27(3):288-292.
王火根,范忠勇,沈宏,等.2006.中国大陆海域须鲸科的新纪录.水产科学,25(2):85-87.
王丕烈.2012.中国鲸类.北京:化学工业出版社,76-81.
杨光,刘海,周开亚,等.2002.用mt DNA序列鉴定一头小布氏鲸标本.动物学杂志,37(4):35-38.
朱小静,何鑫,向余劲攻,等.2018.用mtDNA控制区序列鉴定长须鲸标本.动物学杂志,53(2):234-242.