Non-invasive Observation of Human Tissue Samples with Full Field Optical Coherence Tomography
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摘要
In this work, we report on non-invasive observation of human esophagus, liver, and uterus samples with Full field optical coherence tomography(FFOCT).ln imaging process, fresh human samples were fixed in formalin immediately after excision and then imaged directly without staining and cutting the samples into a serial of thin slices. Tissue microstructures of each type of normal or cancerous tissue as well as their changes with the increase of the depth beneath tissue surface can be identified in the depth-resolved images. The results demonstrate the potential of applications of the en face images in clinic practice.
In this work, we report on non-invasive observation of human esophagus, liver, and uterus samples with Full field optical coherence tomography(FFOCT).ln imaging process, fresh human samples were fixed in formalin immediately after excision and then imaged directly without staining and cutting the samples into a serial of thin slices. Tissue microstructures of each type of normal or cancerous tissue as well as their changes with the increase of the depth beneath tissue surface can be identified in the depth-resolved images. The results demonstrate the potential of applications of the en face images in clinic practice.
In this work, we report on non-invasive observation of human esophagus, liver, and uterus samples with Full field optical coherence tomography(FFOCT).ln imaging process, fresh human samples were fixed in formalin immediately after excision and then imaged directly without staining and cutting the samples into a serial of thin slices. Tissue microstructures of each type of normal or cancerous tissue as well as their changes with the increase of the depth beneath tissue surface can be identified in the depth-resolved images. The results demonstrate the potential of applications of the en face images in clinic practice.
引文
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[22]P.B.Garcia-Allende,I.Amygdalos,H.Dhanapala,et al.,“Morphological analysis of optical coherence tomography images for automated classification of gastrointestinal tissues”,Biomedical Optics Express,Vol.2,No.10,pp.2821-2836,2011.
[23]N.M.Israelsen,M.Maria,M.Mogensen,et al.,“The value of ultrahigh resolution OCT in dermatology-Delineating the dermo-epidermal junction,capillaries in the dermal papillae and vellus hairs,”Biomedical Optics Express,Vol.9,No.5,pp.2240-2265,2018.
[24]C.Magnain,J.C.Augustinack,E.Konukoglu,et al.,“Optical coherence tomography visualizes neurons in human entorhinal cortex,”Neurophotonics,Vol.2,No.1,DOI:10.1117/1.NPh.2.1.015004,2015.
[25]V.J.Srinivasan,H.Radhakrishnan,J.Y.Jiang,et al.,“Optical coherence microscopy for deep tissue imaging of the cerebral cortex with intrinsic contrast,”Optics Express,Vol.20,No.3,pp.2220-2239,2012.
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[27]G.Farhat,V.X.D.Yang,G.J.Czarnot,et al.,“Detecting cell death with optical coherence tomography and envelope statistics”,Journal of Biomedical Optics,Vol.16,No.2,DOI:10.117/1.3544543,2011.
[28]X.Qi,Y.Pan,S.V.Sivak,et al.,“Image analysis for classification of dysplasia in Barrett’s esophagus using endoscopic optical coherence tomography”,Biomedical Optics Express,Vol.1,No.3,pp.825-847,2010.
[29]B.Baumann,A.Woehrer,G.Ricken,et al.,“Visualization of neuritic plaques in Alzheimer’s disease by polarizationsensitive optical coherence microscopy”,Scientific Reports,Vol.7,Article No.43477,2017.
[30]M.Villiger,D.Lorenser,R.A.Mc Laughlin,et al.,“Deep tissue volume imaging of birefringence through fibre-optic needle probes for the delineation of breast tumour”,Scientific Reports,Vol.6,DOI:10.1038/srep28771,2016.
[31]M.Goodwin,B.Br?uer,S.Lewis,et al.,“Quantifying birefringence in the bovine model of early osteoarthritis using polarisation-sensitive optical coherence tomography and mechanical indentation”,Scientific Reports,Vol.8,No.1,DOI:10.1038/s41598-018-25982-9,2018.
[32]Y.Zhu,W.Gao,Y.Zhou,et al.,“Rapid and high-resolution imaging of human liver specimens by full-field optical coherence tomography”,Journal of Biomedical Optics,.Vol.20,No.11,DOI:10.1117/1.JBO.20.11.116010,2015.
[33]A.Bruhat,M.Combrinck,E.Dalimier,et al.,“Full-field optical coherence tomography(FFOCT)for evaluation of endometrial cancer”,Photonic Therapeutics and Diagnostics XI.International Society for Optics and Photonics,Wu Han,Hu Bei,China,Vol.9303,DOI:10.1117/12.2078537,2015.
[34]V.Mazlin,P.Xiao,E.Dalimier,et al.,“In vivo high resolution human corneal imaging using full-field optical coherence tomography”,Biomedical Optics Express,Vol.9,No.2,pp.557-568,2018.
[35]P.Gueye,“Optical coherence tomography:Technology and applications”,Medical Physics,Vol.36,No.10,pp.4842-4843,2009.
[36]A.Safrani and I.Abdulhalim,“Spatial coherence effect on layer thickness determination in narrowband full-field optical coherence tomography”,Applied Optics,Vol.50,No.18,pp.3021-3027,2011.
[37]Y.Zhu,W.Gao and Y.Guo,“A method of improving imaging quality of full-field optical coherence tomography,”Acta Optica Sinica,Vol.35,No.5,pp.285-297,2015.(in Chinese)
[38]Y.Chen,A.D.Aguirre,P.-L.Hsiung,et al.,“Effects of axial resolution improvement on optical coherence tomography(OCT)imaging of gastrointestinal tissues”,Optics Express,Vol.16,No.4,pp.2469-2485,2008.
[2]X.Y.Tang,L.Wang and W.F.Liu,“A comprehensive interpolation for medical slices based on combination of linear and matching interpolation”,Chinese Journal of Electronics,Vol.20,No.1,pp.82-84,2011.
[3]K.Lu,N.He and J.Xue,“A new geometric deformable model for medical image segmentation”,Chinese Journal of Electronics,Vol.18,No.2,pp.200-204,2009.
[4]X.Yuan,L.Wang,W.D.Geng,et al.,“Fully automatic liver segmentation from multi-slice spiral computed tomographic images”,Chinese Journal of Electronics,Vol.18,No.4,pp.677-679,2009.
[5]Q.Guo,L.Wang and S.T.Shen,“Multiple-channel local binary fitting model for medical image segmentation”,Chinese Journal of Electronics,Vol.24,No.4,pp.802-806,2015.
[6]T.Ge,N.Mu and L.Li,“A brain tumor segmentation method based on softmax regression and graph cut”,Acta Electronica Sinica,Vol.45,No.3,pp.644-649,2017.(in Chinese)
[7]X.S.Liu,H.M.Wu,W.B.Xiao,et al.,“Improved double sigmoid function variable step size adaptive algorithm and its application in OCT”,Acta Electronica Sinica,Vol.47,No.1,pp.234-240,2019.(in Chinese)
[8]X.P.Luo and J.Tian,“A modified interactive segmentation of medical image series”,Acta Electronica Sinica,Vol.31,No.1,pp.29-32,2003.(in Chinese)
[9]M.Jain,N.Narula,B.Salamoon,et al.,“Full-field optical coherence tomography for the analysis of fresh unstained human lobectomy specimens”,Journal of Pathology Informatics,Vol.4,No.1,Article No.26,2013.
[10]D.Huang,E.A.Swanson,C.P.Lin,et al.,“Optical coherence tomography”,Science,Vol.254,No.5035,pp.1178-1181,1991.
[11]A.Dubois,L.Vabre,A.C.Boccara,et al.,“Highresolution full-field optical coherence tomography with a Linnik microscope”,Applied Optics,Vol.41,No.4,pp.805-812,2002.
[12]W.Gao and Y.Zhu,“Fractal analysis of en face tomographic images obtained with full field optical coherence tomography”,Annalen der Physik,Vol.529,No.3,DOI:10.1002/andp.201600216,2016.
[13]W.Gao，“Effects of temporal and spatial coherence on resolution in full-field optical coherence tomography”,Journal of Modern Optics,Vol.62,No.21,pp.1764-1774,2015.
[14]W.Gao,“Image contrast reduction mechanism in fullfield optical coherence tomography”,Journal of Microscopy,Vol.261,No.3,pp.199-216,2016.
[15]W.Gao and X.Wu,“Differences between time domain and Fourier domain optical coherence tomography in imaging tissues”,Journal of Microscopy,Vol.268,No.2,pp.119-128,2017.
[16]W Gao，“Fourier spectrum analysis of full-field optical coherence tomography for tissue imaging”,Proceedings of the Royal Society A:Mathematical,Physical and Engineering Science,Vol.471,No.2179,DOI:10.1098/rspa.2015.0099,2015.
[17]W.Gao,“Quantitative depth-resolved microcirculation imaging with optical coherence tomography angiography(Part I):Blood flow velocity imaging”,Microcirculation,Vol.25,No.6,DOI:10.111/micc.12375,2018.
[18]W.Gao,“Quantitative depth-resolved microcirculation imaging with optical coherence tomography angiography(Part II):Microvascular network imaging”,Microcirculation,Vol.25,No.6,DOI:10.111/micc.12376,2018.
[19]L.Liu,J.A Gardecki,S.K Nadkarni,et al.,“Imaging the subcellular structure of human coronary atherosclerosis using micro-optical coherence tomography”,Nature Medicine,Vol.17,No.8,pp.1010-1014,2010.
[20]A.Federici and A.Dubois,“Full-field optical coherence microscopy with optimized ultrahigh spatial resolution”,Optics Letters,Vol.40,No.22,pp.5347-5350,2015.
[21]C.Apelian,F.Harms,O.Thouvenin,et al.,“Dynamic full field optical coherence tomography:subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis,”Biomedical Optics Express,Vol.7,No.4,pp.1511-1524,2016.
[22]P.B.Garcia-Allende,I.Amygdalos,H.Dhanapala,et al.,“Morphological analysis of optical coherence tomography images for automated classification of gastrointestinal tissues”,Biomedical Optics Express,Vol.2,No.10,pp.2821-2836,2011.
[23]N.M.Israelsen,M.Maria,M.Mogensen,et al.,“The value of ultrahigh resolution OCT in dermatology-Delineating the dermo-epidermal junction,capillaries in the dermal papillae and vellus hairs,”Biomedical Optics Express,Vol.9,No.5,pp.2240-2265,2018.
[24]C.Magnain,J.C.Augustinack,E.Konukoglu,et al.,“Optical coherence tomography visualizes neurons in human entorhinal cortex,”Neurophotonics,Vol.2,No.1,DOI:10.1117/1.NPh.2.1.015004,2015.
[25]V.J.Srinivasan,H.Radhakrishnan,J.Y.Jiang,et al.,“Optical coherence microscopy for deep tissue imaging of the cerebral cortex with intrinsic contrast,”Optics Express,Vol.20,No.3,pp.2220-2239,2012.
[26]C.Apelian,F.Harms,O.Thouvenin,et al.,“Dynamic full field optical coherence tomography:Subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis”,Biomedical Optics Express,Vol.7,No.4,pp.1511-1524,2016.
[27]G.Farhat,V.X.D.Yang,G.J.Czarnot,et al.,“Detecting cell death with optical coherence tomography and envelope statistics”,Journal of Biomedical Optics,Vol.16,No.2,DOI:10.117/1.3544543,2011.
[28]X.Qi,Y.Pan,S.V.Sivak,et al.,“Image analysis for classification of dysplasia in Barrett’s esophagus using endoscopic optical coherence tomography”,Biomedical Optics Express,Vol.1,No.3,pp.825-847,2010.
[29]B.Baumann,A.Woehrer,G.Ricken,et al.,“Visualization of neuritic plaques in Alzheimer’s disease by polarizationsensitive optical coherence microscopy”,Scientific Reports,Vol.7,Article No.43477,2017.
[30]M.Villiger,D.Lorenser,R.A.Mc Laughlin,et al.,“Deep tissue volume imaging of birefringence through fibre-optic needle probes for the delineation of breast tumour”,Scientific Reports,Vol.6,DOI:10.1038/srep28771,2016.
[31]M.Goodwin,B.Br?uer,S.Lewis,et al.,“Quantifying birefringence in the bovine model of early osteoarthritis using polarisation-sensitive optical coherence tomography and mechanical indentation”,Scientific Reports,Vol.8,No.1,DOI:10.1038/s41598-018-25982-9,2018.
[32]Y.Zhu,W.Gao,Y.Zhou,et al.,“Rapid and high-resolution imaging of human liver specimens by full-field optical coherence tomography”,Journal of Biomedical Optics,.Vol.20,No.11,DOI:10.1117/1.JBO.20.11.116010,2015.
[33]A.Bruhat,M.Combrinck,E.Dalimier,et al.,“Full-field optical coherence tomography(FFOCT)for evaluation of endometrial cancer”,Photonic Therapeutics and Diagnostics XI.International Society for Optics and Photonics,Wu Han,Hu Bei,China,Vol.9303,DOI:10.1117/12.2078537,2015.
[34]V.Mazlin,P.Xiao,E.Dalimier,et al.,“In vivo high resolution human corneal imaging using full-field optical coherence tomography”,Biomedical Optics Express,Vol.9,No.2,pp.557-568,2018.
[35]P.Gueye,“Optical coherence tomography:Technology and applications”,Medical Physics,Vol.36,No.10,pp.4842-4843,2009.
[36]A.Safrani and I.Abdulhalim,“Spatial coherence effect on layer thickness determination in narrowband full-field optical coherence tomography”,Applied Optics,Vol.50,No.18,pp.3021-3027,2011.
[37]Y.Zhu,W.Gao and Y.Guo,“A method of improving imaging quality of full-field optical coherence tomography,”Acta Optica Sinica,Vol.35,No.5,pp.285-297,2015.(in Chinese)
[38]Y.Chen,A.D.Aguirre,P.-L.Hsiung,et al.,“Effects of axial resolution improvement on optical coherence tomography(OCT)imaging of gastrointestinal tissues”,Optics Express,Vol.16,No.4,pp.2469-2485,2008.