应用脉冲标记法对杉木富集~(13)C技术的初步研究
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摘要
通过盆栽试验对杉木幼苗进行13C同位素标记,并对杉木不同器官光合产物的分配进行研究,为木本植物的同位素标记与光合分配研究提供参考依据。结果表明,未标记杉木针叶、枝干的δ13C值随时间呈下降趋势,根呈现先下降后上升的趋势;标记后,杉木各器官δ13C值随时间呈现明显上升,达到高点后有一定下降。光合碳分配到杉木不同器官间的Atom%13C存在差异,大致呈现出当年生针叶最大,一年生针叶最小,根、枝干居中。13C标记使杉木针叶、枝干、根的δ13C由-25.185‰、-24.689‰、-25.326‰升为116.737‰、106.800‰、124.080‰。经过13C标记可获得富集13C的木本植物材料,可为研究土壤碳组分周转提供试验材料。
A pot experiment was conducted,by13 C pulse labeling technique,to characterize the enrichment of13 C in C. lanceolata plant and analyze the allocation of photosynthesis products among Chinese fir organs. The study aimed to provide a reference which about isotope labeling of woody plants and the allocation of photosynthesis carbon among plants results showed that the values of δ13C in needle and stem of unlabeled C. lanceolata decreased with time,and the values in root decreased at the beginning and then increased. The values of δ13C in different organs of labeled C. lanceolata initially increased and then decreased after the summit. The values of Atom%13C of photosynthetic carbon allocation were different in C. lanceolata organs,with the largest value of Atom%13C in current-needle,the smallest in 1-yearold needle,and the values in stem and root in the middle. After13 C pulse labeling,the stable carbon isotope ratios( δ13C values) in needle,stem and root increased from-25. 185‰,-24. 689‰,-25. 326‰ to 116. 737‰,106. 800‰,124. 080‰,respectively. The above results indicated that plant materials which enriched13 C could be obtained with13 C pulse labeling technique,and could be useful for the study of turnover dynamics of soil carbon components.
A pot experiment was conducted,by13 C pulse labeling technique,to characterize the enrichment of13 C in C. lanceolata plant and analyze the allocation of photosynthesis products among Chinese fir organs. The study aimed to provide a reference which about isotope labeling of woody plants and the allocation of photosynthesis carbon among plants results showed that the values of δ13C in needle and stem of unlabeled C. lanceolata decreased with time,and the values in root decreased at the beginning and then increased. The values of δ13C in different organs of labeled C. lanceolata initially increased and then decreased after the summit. The values of Atom%13C of photosynthetic carbon allocation were different in C. lanceolata organs,with the largest value of Atom%13C in current-needle,the smallest in 1-yearold needle,and the values in stem and root in the middle. After13 C pulse labeling,the stable carbon isotope ratios( δ13C values) in needle,stem and root increased from-25. 185‰,-24. 689‰,-25. 326‰ to 116. 737‰,106. 800‰,124. 080‰,respectively. The above results indicated that plant materials which enriched13 C could be obtained with13 C pulse labeling technique,and could be useful for the study of turnover dynamics of soil carbon components.
引文
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[20]平晓燕,周广胜,孙敬松.植物光合产物分配及其影响因子研究进展[J].植物生态学报,2010,34(1):100-111
[21]贺海波,李彦.干旱、盐胁迫条件下两种盐生植物生物量分配对策的研究[J].干旱区研究,2008,25(2):242-247
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[23]Lu Y H,Watanabe A,Kimura M.Carbon dynamics of rhizodeposits,root-and shoot-residues in a rice soil[J].Soil Biology and Biochemistry,2003,35(9):1223-1230
[24]Simard S W,Durall D M,Jones M D.Carbon allocation and carbon transfer between Betula papyrifera and Pseudotsuga menziesii seedlings using a13C pulse-labeling method[J].Plant and Soil,1997,191(1):41-55
[25]Glaser B,Benesch M,Dippold M,Zech W.In situ15N and13C labelling of indigenous and plantation tree species in a tropical mountain forest(Munessa,Ethiopia)for subsequent litter and soil organic matter turnover studies[J].Organic Geochemistry,2012,42(12):1461-1469
[26]Bromand S,Whalen J K,Janzen H H,Schjoerring J K,Ellert B H.A pulse-labeling method to generate13C-enriched plant materials[J].Plant and Soil,2001,235(2):253-257
[27]刘微,吕豪豪,陈英旭,吴伟祥.稳定碳同位素技术在土壤-植物系统碳循环中的应用[J].应用生态学报,2008,19(3):674-680
[2]王周锋,刘卫国,邓西平.冬小麦生长期氮同位素组成对温度的响应[J].核农学报,2011,25(1):11-14
[3]安婷婷,王景宽,李双异,付时丰,裴久渤,李慧.用13C脉冲标记方法研究施肥与地膜覆盖对玉米光合碳分配的影响[J].土壤通报,2013,50(5):948-955
[4]Li Z,Yagi K.Rice root-derived carbon input and its effect on decomposition of old soil carbon pool under elevated CO2[J].Soil Biology and Biochemistry,2004,36(12):1967-1973
[5]聂三安,周萍,葛体达,童成立,肖和艾,吴金水,张杨珠.水稻光合同化碳向土壤有机碳库输入的定量研究:14C连续标记法[J].环境科学,2012,33(4):1346-1351
[6]Lattanzi F A,Berone G D,Feneis W,Schnyder H.13C-labeling shows the effect of hierarchy on the carbon gain of individuals and functional groups in dense field stands[J].Ecology,2012,93(1):169-179
[7]Pumpanen J S,Heinonsalo J,Rasilo T,Hurme K R,Ilvesniemi H.Carbon balance and allocation of assimilated CO2in Scots pine,Norway spruce,and Silver birch seedlings determined with gas exchange measurements and14C pulse labeling[J].Trees,2009(3):611-621
[8]Kagawa A,Sugimoto A,Maximov T C.Seasonal course of translocation,storage and remobilization of13C pulse-labelled photoassimilate in naturally growing Larix gmelinii saplings[J].New Phytologist,2006,171(4):793-804
[9]方晰,田大伦.杉木人工林C库与C吸存的动态研究[J].广西植物,2006,26(5):516-522
[10]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,1999:13-169
[11]尹云锋,杨玉盛,高人,马红亮,卢茜.植物富集13C标记技术的初步研究[J].土壤学报,2010,47(4):790-793
[12]王丽霞,汪卫国,李心清,郭兰兰.中国北方干旱半干旱区表土的有机质碳同位素、磁化率与年降水量的关系[J].干旱区地理,2005,28(3):309-315
[13]Niklas K J.A phyletic perspective on the allometry of plant biomasspartitioning patterns and functionally equivalent organ-categories[J].New Phytologist,2006,171(1):27-40
[14]韦莉莉,张小全,侯振宏,徐德应,余雪标.杉木苗木光合作用及其产物分配对水分胁迫的响应[J].植物生态学报,2005,29(3):394-402
[15]陈俊伟,倪竹如,刘智宏,付志坚.利用14C示踪法研究杉木光合产物分配和杉木根系分泌物[J].核农学报,1994,8(3):167-171
[16]张小全,徐德应.温度对杉木中龄林针叶光合生理生态的影响[J].林业科学,2002,38(3):27-33
[17]乔云发,韩晓增,赵兰坡.长期定量施肥对玉米光合碳分配的影响[J].水土保持学报,2010,24(4):208-212
[18]韩兴国,严昌荣,陈灵芝,梅旭荣.暖温带地区几种木本植物碳稳定同位素的特点[J].应用生态学报,2000,11(4):497-500
[19]Vogel J G,Bond-Lamberty B P,Schuur E A G,Gower S T,Mack M C,O’Connell K E B,Valentine D W,Ruess R W.Carbon allocation in boreal black spruce forests across regions varying in soil temperature and precipitation[J].Global Change Biology,2008,14(7):1503-1516
[20]平晓燕,周广胜,孙敬松.植物光合产物分配及其影响因子研究进展[J].植物生态学报,2010,34(1):100-111
[21]贺海波,李彦.干旱、盐胁迫条件下两种盐生植物生物量分配对策的研究[J].干旱区研究,2008,25(2):242-247
[22]许育彬,沈玉芳,李世清.分期施氮与CO2浓度升高对小麦光合及其物质积累和产量的互作效应[J].西北植物学报,2012,32(5):1007-1012
[23]Lu Y H,Watanabe A,Kimura M.Carbon dynamics of rhizodeposits,root-and shoot-residues in a rice soil[J].Soil Biology and Biochemistry,2003,35(9):1223-1230
[24]Simard S W,Durall D M,Jones M D.Carbon allocation and carbon transfer between Betula papyrifera and Pseudotsuga menziesii seedlings using a13C pulse-labeling method[J].Plant and Soil,1997,191(1):41-55
[25]Glaser B,Benesch M,Dippold M,Zech W.In situ15N and13C labelling of indigenous and plantation tree species in a tropical mountain forest(Munessa,Ethiopia)for subsequent litter and soil organic matter turnover studies[J].Organic Geochemistry,2012,42(12):1461-1469
[26]Bromand S,Whalen J K,Janzen H H,Schjoerring J K,Ellert B H.A pulse-labeling method to generate13C-enriched plant materials[J].Plant and Soil,2001,235(2):253-257
[27]刘微,吕豪豪,陈英旭,吴伟祥.稳定碳同位素技术在土壤-植物系统碳循环中的应用[J].应用生态学报,2008,19(3):674-680