产地加工与炮制一体化工艺对秦皮饮片抗炎作用的影响
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摘要
目的:比较秦皮饮片产地加工与炮制一体化加工工艺与传统加工方法的抗炎作用。方法:采用角叉菜胶诱导大鼠足肿胀模型,研究不同工艺的秦皮饮片水提物的抗炎消肿作用。采用高效液相测定2种秦皮饮片所含主要化学成分。结果:与空白组比较,2种秦皮饮片水提物均可降低大鼠足肿胀度,改善各项炎症指标,但以一体化加工秦皮饮片的抗炎消肿作用更为显著。一体化加工秦皮饮片中秦皮甲素、秦皮苷、秦皮乙素、秦皮素等4种主要有效成分的含量均高于传统方式加工的秦皮饮片,这四种香豆素类成分在一体化秦皮饮片的总量约为传统秦皮饮片水提物的1.5倍。结论:秦皮饮片一体化加工与传统加工在抗炎功效上作用相似,且具有减少有效成分流失的优越性,值得推广应用。
Objective: To compare the anti-inflammatory effect of Fraxini Cortex pieces between integrated production process and traditional processing method. Methods: The model of rat paw swelling induced by carrageenan was used to study the anti-inflammatory and swelling reliving effects of water extracts of Cortex Fraxini with different methods. The main chemical components of the 2 kinds of Cortex Fraxini herbal pieces were determined by high performance liquid phase. Results: Compared with the blank group, the water extracts of the 2 kinds of Cortex Fraxini could reduce the swelling of the rats and improve the various indicators of inflammation. However, the anti-inflammatory and swelling reliving effects of the integrated processing of Cortex Fraxini were more significant. The contents of 4 main active ingredients of esculine, fraxin, aesculetin and fraxetin in the integrated processing of Cortex Fraxini were higher than that of the traditionally processed Cortex Fraxini. The total amount of 4 kinds of coumarins in the integrated Cortex Fraxini was about 1.5 times that of the traditionally processed water extract of Cortex Fraxini. Conclusion: The integrated processing and traditional processing of Cortex Fraxini have similar effects on anti-inflammatory effects, and have the superiority of reducing the loss of active ingredients, which is worthy of popularization and application.
Objective: To compare the anti-inflammatory effect of Fraxini Cortex pieces between integrated production process and traditional processing method. Methods: The model of rat paw swelling induced by carrageenan was used to study the anti-inflammatory and swelling reliving effects of water extracts of Cortex Fraxini with different methods. The main chemical components of the 2 kinds of Cortex Fraxini herbal pieces were determined by high performance liquid phase. Results: Compared with the blank group, the water extracts of the 2 kinds of Cortex Fraxini could reduce the swelling of the rats and improve the various indicators of inflammation. However, the anti-inflammatory and swelling reliving effects of the integrated processing of Cortex Fraxini were more significant. The contents of 4 main active ingredients of esculine, fraxin, aesculetin and fraxetin in the integrated processing of Cortex Fraxini were higher than that of the traditionally processed Cortex Fraxini. The total amount of 4 kinds of coumarins in the integrated Cortex Fraxini was about 1.5 times that of the traditionally processed water extract of Cortex Fraxini. Conclusion: The integrated processing and traditional processing of Cortex Fraxini have similar effects on anti-inflammatory effects, and have the superiority of reducing the loss of active ingredients, which is worthy of popularization and application.
引文
1国家药典委员会.中华人民共和国药典(一部).北京:中国医药科技出版社, 2015:271.
2 方莲花,吕扬,杜冠华.秦皮的药理作用研究进展.中国中药杂志,2008, 33(23):2732-2734.
3 聂安政,林志健,张冰.秦皮化学成分和药理作用研究进展.中草药,2016, 47(18):3332-3341.
4 Yang L, Liu Y, Zu Y G, et al. Optimize the process of ionic liquidbased ultrasonic-assisted extraction of aesculin and aesculetin from Cortex Fraxini by response surface methodology. Chem Eng J, 2011, 175(1):539-547.
5 邓严冰,甘我挺,郭宝林,等.桂枝地骨皮等5种藤茎及皮类药材商品电子交易规格等级标准.中国现代中药, 2016, 18(11):1410-1415.
6 黎丹,姜巧芳,白吉庆,等.树龄对秦岭白蜡树树皮中秦皮甲素、秦皮乙素与秦皮素含量的影响.中南药学, 2016, 14(1):60-62.
7 王景媛,翟思程,王昌利,等.秦皮产地加工与炮制一体化技术研究.陕西中医学院学报, 2017, 40(5):79-81.
8 汤丽芝,史亚军,刘继平,等.秦皮提取物及秦皮甲素对大鼠类风湿关节炎的保护作用.中国药业, 2016, 25(16):27-30.
9 沈淑洁,水素芳,肖炳坤,等.基于~1H-NMR及LC-MS技术研究金铃子散对炎症大鼠模型调节机制的影响.中国中药杂志, 2017, 42(2):363-369.
10 白小军,杨锋,呼睿,等.消肿止痛膏对足肿胀模型大鼠足肿胀度的影响.陕西中医, 2017, 38(8):1144-1145.
11 冯伟红,王智民,张启伟,等.一测多评法测定秦皮药材与饮片中香豆素类成分的含量.中国中药杂志, 2011, 36(13):1782-1789.
12 李晓尧,张丹,汤丽芝,等. HPLC法同时测定秦皮中四种成分含量的研究.陕西中医, 2016, 37(9):1238-1240.
13 谢绍锋,曹雯,胡咏新,等.芪葵颗粒对2型糖尿病肾病患者蛋白尿及炎症因子的影响.世界科学技术-中医药现代化, 2017, 19(1):149-153.
14 顾亮亮,付国惠,张保朝.天麻钩藤颗粒联合左旋多巴治疗帕金森病的疗效及对血清炎性因子和SOD、MDA、Cys-C的影响.中西医结合心脑血管病杂志, 2018, 16(1):95-98.
15 罗玫,刘芳,邹建东,等.秦皮总香豆素对原发性急性痛风性关节炎止痛效应的探索性临床试验.中国临床药理学与治疗学, 2005(4):475-478.
16 江芮,吕浩,李申,等.秦皮素抑制NF-κB/COX-2信号通路改善PC12细胞损伤.沈阳药科大学学报, 2017, 34(6):513-518.
17 杨欢,程笑,王月华,等.秦皮甲素对角叉菜胶致小鼠足肿胀及炎症的作用.中国新药杂志, 2016, 25(20):2319-2322.
18 许冉达,邵天宇,贾绍华.秦皮乙素对荷瘤小鼠红细胞免疫功能的影响.哈尔滨商业大学学报(自然科学版), 2016, 32(2):146-149+153.
2 方莲花,吕扬,杜冠华.秦皮的药理作用研究进展.中国中药杂志,2008, 33(23):2732-2734.
3 聂安政,林志健,张冰.秦皮化学成分和药理作用研究进展.中草药,2016, 47(18):3332-3341.
4 Yang L, Liu Y, Zu Y G, et al. Optimize the process of ionic liquidbased ultrasonic-assisted extraction of aesculin and aesculetin from Cortex Fraxini by response surface methodology. Chem Eng J, 2011, 175(1):539-547.
5 邓严冰,甘我挺,郭宝林,等.桂枝地骨皮等5种藤茎及皮类药材商品电子交易规格等级标准.中国现代中药, 2016, 18(11):1410-1415.
6 黎丹,姜巧芳,白吉庆,等.树龄对秦岭白蜡树树皮中秦皮甲素、秦皮乙素与秦皮素含量的影响.中南药学, 2016, 14(1):60-62.
7 王景媛,翟思程,王昌利,等.秦皮产地加工与炮制一体化技术研究.陕西中医学院学报, 2017, 40(5):79-81.
8 汤丽芝,史亚军,刘继平,等.秦皮提取物及秦皮甲素对大鼠类风湿关节炎的保护作用.中国药业, 2016, 25(16):27-30.
9 沈淑洁,水素芳,肖炳坤,等.基于~1H-NMR及LC-MS技术研究金铃子散对炎症大鼠模型调节机制的影响.中国中药杂志, 2017, 42(2):363-369.
10 白小军,杨锋,呼睿,等.消肿止痛膏对足肿胀模型大鼠足肿胀度的影响.陕西中医, 2017, 38(8):1144-1145.
11 冯伟红,王智民,张启伟,等.一测多评法测定秦皮药材与饮片中香豆素类成分的含量.中国中药杂志, 2011, 36(13):1782-1789.
12 李晓尧,张丹,汤丽芝,等. HPLC法同时测定秦皮中四种成分含量的研究.陕西中医, 2016, 37(9):1238-1240.
13 谢绍锋,曹雯,胡咏新,等.芪葵颗粒对2型糖尿病肾病患者蛋白尿及炎症因子的影响.世界科学技术-中医药现代化, 2017, 19(1):149-153.
14 顾亮亮,付国惠,张保朝.天麻钩藤颗粒联合左旋多巴治疗帕金森病的疗效及对血清炎性因子和SOD、MDA、Cys-C的影响.中西医结合心脑血管病杂志, 2018, 16(1):95-98.
15 罗玫,刘芳,邹建东,等.秦皮总香豆素对原发性急性痛风性关节炎止痛效应的探索性临床试验.中国临床药理学与治疗学, 2005(4):475-478.
16 江芮,吕浩,李申,等.秦皮素抑制NF-κB/COX-2信号通路改善PC12细胞损伤.沈阳药科大学学报, 2017, 34(6):513-518.
17 杨欢,程笑,王月华,等.秦皮甲素对角叉菜胶致小鼠足肿胀及炎症的作用.中国新药杂志, 2016, 25(20):2319-2322.
18 许冉达,邵天宇,贾绍华.秦皮乙素对荷瘤小鼠红细胞免疫功能的影响.哈尔滨商业大学学报(自然科学版), 2016, 32(2):146-149+153.