桔梗不同器官中多糖的分布与动态积累研究
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摘要
以D-无水葡萄糖为对照品,采用蒽酮-硫酸比色法测定不同生长年限桔梗中总多糖的含量,研究桔梗一年生长过程中不同器官总多糖的分布和动态积累规律。结果表明,D-无水葡萄糖在0. 002 5～0. 015 mg/mL范围内与吸光度线性关系良好(r=0. 999 6)。不去皮桔梗药材中多糖含量低于去皮桔梗。不去皮桔梗药材中总多糖含量随着生长年限的增长呈现先升再降再升的变化趋势,5年生多糖含量最高。去皮桔梗药材中总多糖的含量随着生长年限的延长呈现下降再略上升的趋势,即2～4年不断降低,至第5年略有上升。桔梗中多糖主要分布在根部,刚发芽时多糖含量最低,盛花期时多糖含量最高。该研究建立了桔梗中总多糖含量的测定方法,为桔梗资源的综合利用提供了一定的依据。
To determine the content of polysaccharides and to explore the distribution and dynamic accumulation of polysaccharides in Platycodon grandiflorum at various growth ages and in different organs.Through adopting method,the content of total polysaccharide in P.grandiflorum samples at various growth ages and in different organs was determined with glucose as reference control.The glucose contents showed the fine linear relation with absorbance in the range of 0. 002 5 ～ 0. 015 mg/mL,the relevance modulus r = 0. 999 6.The content of polysaccharide in unpeeled Platycodon is significantly lower than peeled polysaccharide.In the unpeeled p. grandiflorum,the content of total polysaccharide accumulation was significantly increased and then gradually decreased,increased finally and the 5-year-old is the highest.The content of total polysaccharide in the peeled P. grandiflorum decreased and then increased slightly,that is,it decreased continuously in 2 to 4 years,and increased slightly in the fifth year.The polysaccharides in P.grandiflorum are mainly distributed in the roots.The content of polysaccharides is the lowest when germination,and the highest in the flowering stage. This study established a method for determination of total polysaccharide content in platycodon grandiflorum,which provided a basis for comprehensive utilization of P.grandiflorum resources.
To determine the content of polysaccharides and to explore the distribution and dynamic accumulation of polysaccharides in Platycodon grandiflorum at various growth ages and in different organs.Through adopting method,the content of total polysaccharide in P.grandiflorum samples at various growth ages and in different organs was determined with glucose as reference control.The glucose contents showed the fine linear relation with absorbance in the range of 0. 002 5 ～ 0. 015 mg/mL,the relevance modulus r = 0. 999 6.The content of polysaccharide in unpeeled Platycodon is significantly lower than peeled polysaccharide.In the unpeeled p. grandiflorum,the content of total polysaccharide accumulation was significantly increased and then gradually decreased,increased finally and the 5-year-old is the highest.The content of total polysaccharide in the peeled P. grandiflorum decreased and then increased slightly,that is,it decreased continuously in 2 to 4 years,and increased slightly in the fifth year.The polysaccharides in P.grandiflorum are mainly distributed in the roots.The content of polysaccharides is the lowest when germination,and the highest in the flowering stage. This study established a method for determination of total polysaccharide content in platycodon grandiflorum,which provided a basis for comprehensive utilization of P.grandiflorum resources.
引文
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[11]张雁.不同年限桔梗与土壤水分时空分布的响应研究[J].农业开发与装备,2013,(9):61-62.
[12]孙清华,刘道杰.用于测定糖的衍生试剂的研究进展[J].化学试剂,2005,27(11):653-656.
[13]祝丽香,王建华,孙印石,等.桔梗开花后可溶性糖和淀粉分配特性的研究[J].园艺学报,2010,37(2):319-324.
[14]张文静,王鹏,陈香香,等.桔梗辣椒间作对桔梗根系生长及产量品质的影响[J].中国中药杂志,2018,(6):1 111-1 117.
[15]LYNCH D J,MATAMALA R,IVERSEN C M,et al.Stored carbon partly fuels fine-root respiration but is not used for production of new fine roots[J]. New Phytol.,2013,199(2):420-430.
[16]吴珍,潘婷婷,王新军.光质对桔梗根生长及有效成分积累的影响[J].西北林学院学报,2018,33(2):129-133.
[2]GAO W Y,LI Z L,XIAO P G.Advances in research of platycodon grandiflorum[J]. Res. Pract. Chin. Med.,1996,10(2):48-50.
[3]李喜凤,杜云锋,谢新年,等.不同产地桔梗药材HPLC指纹图谱及桔梗皂苷D含量测定研究[J].中成药,2010,32(4):529-532.
[4]吴梅青,刘佳佳.桔梗化学成分研究进展[J].黑龙江医药,2007,20(5):443-446.
[5]贾林,陆金健,卢德赵,等.桔梗多糖的分离纯化与含量测定[J].中国农学通报,2011,27(17):83-86.
[6]维彩云.桔梗多糖提取纯化和生物活性研究进展[J].农业科技与装备,2016,(11):67-68.
[7]杨晓杰,于侃超,李娜,等.桔梗多糖抗疲劳活性研究[J].天然产物研究与开发,2015,27(3):459-461.
[8]ZHAO X,WANG Y,YAN P,et al.Effects of polysaccharides from platycodon grandiflorum on immunity-enhancing activity in vitro[J].Molecules,2017,22(11):1 918.
[9]赵祥升,赵禹凯,师凤华.不同加工方法对桔梗根中多糖含量的影响[J].中国现代应用药学,2017,11(27):983-986.
[10]杨盼盼,李尚擘,李焰焰,等.不同种植年限桔梗的农艺性状与相关环境因子分析[J].生物学杂志,2016,33(3):66-69.
[11]张雁.不同年限桔梗与土壤水分时空分布的响应研究[J].农业开发与装备,2013,(9):61-62.
[12]孙清华,刘道杰.用于测定糖的衍生试剂的研究进展[J].化学试剂,2005,27(11):653-656.
[13]祝丽香,王建华,孙印石,等.桔梗开花后可溶性糖和淀粉分配特性的研究[J].园艺学报,2010,37(2):319-324.
[14]张文静,王鹏,陈香香,等.桔梗辣椒间作对桔梗根系生长及产量品质的影响[J].中国中药杂志,2018,(6):1 111-1 117.
[15]LYNCH D J,MATAMALA R,IVERSEN C M,et al.Stored carbon partly fuels fine-root respiration but is not used for production of new fine roots[J]. New Phytol.,2013,199(2):420-430.
[16]吴珍,潘婷婷,王新军.光质对桔梗根生长及有效成分积累的影响[J].西北林学院学报,2018,33(2):129-133.