高效液相法测定蒙成药健脾五味丸中没食子酸的含量
|
摘要
目的建立健脾五味丸中没食子酸的含量测定方法。方法高效液相色谱法。Diamonsil C18柱(250mm×4.6mm,5μm);流动相为甲醇-0.1%磷酸(7:93);流速为1.0mL·min-1;检测波长为270nm;柱温30℃。结果没食子酸在45 ng～452 ng范围内与峰面积积分值呈良好的线性关系(γ=1.0000),回归方程为y=3.8164x-6.7537,平均回收率为98.0%,RSD%=1.23%(n=9)。结论该方法精密、准确、复性好,可作为健脾五味丸中没食子酸的含量测定方法。
Objective To establish the determination method of gallic acid in Jianpi Wuwei Pills.Methods Diamonsil C18 column(250 mm×4.6 mm, 5 μm) was used in HPLC assay; mobile phase was methanol-0.1% phosphoric acid(7:93); flow rate was 1.0 mL·min-1; detection wavelength was 270 nm;column temperature was 30℃. Results The Calibration curve of gallic acid was linear in the range of45 ng～452 ng(γ=1.0000),the average recovery rate was 98.0%,the regresstion equation was y=3.8164 x-6.7537,RSD was 1.23%(n=9). Conclusion The method was accurate,precise and had good reproducibility, which can be used as determination method of gallic acid in Jianpi Wuwei Pills.
Objective To establish the determination method of gallic acid in Jianpi Wuwei Pills.Methods Diamonsil C18 column(250 mm×4.6 mm, 5 μm) was used in HPLC assay; mobile phase was methanol-0.1% phosphoric acid(7:93); flow rate was 1.0 mL·min-1; detection wavelength was 270 nm;column temperature was 30℃. Results The Calibration curve of gallic acid was linear in the range of45 ng～452 ng(γ=1.0000),the average recovery rate was 98.0%,the regresstion equation was y=3.8164 x-6.7537,RSD was 1.23%(n=9). Conclusion The method was accurate,precise and had good reproducibility, which can be used as determination method of gallic acid in Jianpi Wuwei Pills.
引文
[1]霍生青.HPLC测定十三味菥冥丸中的没食子酸[J].华西药学杂志,2012,27(1):92-93.
[2]张雅,曹蔚,谢艳华,等.HPLC法测定复方烧伤膏中没食子酸的含量[J].西北药学杂志,2012,27(5):425-426.
[3]诸明娜,陆兔林,毛春芹,等. H P L C测定山茱萸不同炮制品中5-羟甲基糠醛和没食子酸的含量[J].中国实验方剂学杂志,2012,18(7):64-67.
[4]李小曲.RP-HPLC法测定诃子五味胶囊中没食子酸的含量[J].中国药师,2010,13(2):219-220.
[5]阿依努尔·阿木提,司马义江·阿布都热西提.HPLC法测定维吾尔药制剂清涩比黑马尔江散中没食子酸的含量[J].中国民族民间医药,2012,21(15):11-12.
[6]诸明娜,陆兔林,毛春芹,等. H P L C测定山茱萸不同炮制品中5-羟甲基糠醛和没食子酸的含量[J].中国实验方剂学杂志,2012,18(7):64-67.
[7]张雅,曹蔚,谢艳华,等.HPLC法测定复方烧伤膏中没食子酸的含量[J].西北药学杂志,2012,27(5):425-426.
[2]张雅,曹蔚,谢艳华,等.HPLC法测定复方烧伤膏中没食子酸的含量[J].西北药学杂志,2012,27(5):425-426.
[3]诸明娜,陆兔林,毛春芹,等. H P L C测定山茱萸不同炮制品中5-羟甲基糠醛和没食子酸的含量[J].中国实验方剂学杂志,2012,18(7):64-67.
[4]李小曲.RP-HPLC法测定诃子五味胶囊中没食子酸的含量[J].中国药师,2010,13(2):219-220.
[5]阿依努尔·阿木提,司马义江·阿布都热西提.HPLC法测定维吾尔药制剂清涩比黑马尔江散中没食子酸的含量[J].中国民族民间医药,2012,21(15):11-12.
[6]诸明娜,陆兔林,毛春芹,等. H P L C测定山茱萸不同炮制品中5-羟甲基糠醛和没食子酸的含量[J].中国实验方剂学杂志,2012,18(7):64-67.
[7]张雅,曹蔚,谢艳华,等.HPLC法测定复方烧伤膏中没食子酸的含量[J].西北药学杂志,2012,27(5):425-426.