高效液相色谱法测定肠炎灌肠液中盐酸小檗碱含量
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摘要
目的建立测定肠炎灌肠液中盐酸小檗碱含量的高效液相色谱(HPLC)法。方法色谱柱为Kromasil 100-5 C18柱(250 mm×4. 6 mm,5μm),流动相为乙腈-0. 1%磷酸溶液(50∶50,V/V,每100 m L加十二烷基磺酸钠0. 1 g),流速为1 m L/min,检测波长为265 nm,柱温为40℃,进样量为10μL。结果盐酸小檗碱进样质量浓度在6. 72～215. 02 g/L范围内与峰面积线性关系良好,精密度、稳定性、重复性试验结果的RSD均小于2%,平均加样回收率为101. 91%,RSD为1. 53%(n=6)。结论该方法准确、灵敏,可用于肠炎灌肠液的质量控制。
Objective To establish an HPLC method for the content determination of berberine hydrochloride in enterocolenteral enema.Methods The chromatographic column was Kromasil 100-5 C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-0. 1% phosphoric acid solution( 50 ∶ 50,V/V,0. 1 g sodium dodecylsulphate was added into the 100 m L mobile phase),the flow rate was1 m L/min,the detection wavelength was 265 nm,the column temperature was 40 ℃ and the sample size was 10 μL. Results Berberine hydrochloride showed good relationship in the range of 6. 72-215. 02 g/L. RSDs of precision,repeatability and stability tests were less than 2%,the average recovery rate was 101. 91%,RSD was 1. 53%( n = 6). Conclusion The method is accurate,sensitive and can be used for the quality control of enterocolenteral enema.
Objective To establish an HPLC method for the content determination of berberine hydrochloride in enterocolenteral enema.Methods The chromatographic column was Kromasil 100-5 C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-0. 1% phosphoric acid solution( 50 ∶ 50,V/V,0. 1 g sodium dodecylsulphate was added into the 100 m L mobile phase),the flow rate was1 m L/min,the detection wavelength was 265 nm,the column temperature was 40 ℃ and the sample size was 10 μL. Results Berberine hydrochloride showed good relationship in the range of 6. 72-215. 02 g/L. RSDs of precision,repeatability and stability tests were less than 2%,the average recovery rate was 101. 91%,RSD was 1. 53%( n = 6). Conclusion The method is accurate,sensitive and can be used for the quality control of enterocolenteral enema.
引文
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[11]李静,钱江.高效液相色谱法同时测定复方黄连素片中芍药苷和盐酸小檗碱含量[J].中国药业,2016,25(7):61-63.
[12]彭晶玲,李芳平.高效液相色谱法测定糖肾康丸中盐酸小檗碱含量[J].亚太传统医药,2016,12(7):29-31.
[13]梁凤娟.高效液相色谱法测定尿酸胶囊中盐酸小檗碱的含量[J].临床和实验医学杂志,2007,6(8):32-33.
[2]徐红,吕灵仪,陈晓辉,等. 5-氨基水杨酸肠溶片治疗慢性肠炎的疗效[J].中国医药科学,2013,3(4):68-69.
[3]李茜.左氧氟沙星联合甲硝唑和山莨菪碱治疗慢性肠炎的临床观察[J].甘肃医药,2013,32(12):892-893.
[4]余腾江,陈卫东,李五生,等.白头翁汤联合康复新液灌肠治疗湿热型溃疡性结肠炎43例临床观察[J].云南中医中药杂志,2017,38(6):52-53.
[5]国家药典委员会.中华人民共和国药典(四部)[M].北京:中国医药科技出版社,2015:374-376.
[6]李彩虹,周克元.黄连活性成分的作用及机制研究进展[J].时珍国医国药,2010,21(2):466-468.
[7]陈阳峰,钟晓红.黄柏的药理作用及其活性成分提取[J].作物研究,2015,29(5):564-568.
[8]陈良.黄金膏中盐酸小檗碱的定性鉴别与含量测定[J].中国药房,2015,26(30):4262-4263.
[9]李茂森.复方黄连素片中盐酸小檗碱含量测定方法改进[J].中国药业,2013,22(14):68-69.
[10]刘佳,陆雪萍. HPLC测定止泻保童颗粒中盐酸小檗碱的含量[J].云南中医学院学报,2016,39(2):37-39.
[11]李静,钱江.高效液相色谱法同时测定复方黄连素片中芍药苷和盐酸小檗碱含量[J].中国药业,2016,25(7):61-63.
[12]彭晶玲,李芳平.高效液相色谱法测定糖肾康丸中盐酸小檗碱含量[J].亚太传统医药,2016,12(7):29-31.
[13]梁凤娟.高效液相色谱法测定尿酸胶囊中盐酸小檗碱的含量[J].临床和实验医学杂志,2007,6(8):32-33.