抗感RILs株系(岗46B/A232)主要农艺性状与三化螟抗性的相关分析
|
摘要
【目的】明确三化螟产卵趋性和幼虫取食趋性与水稻植株主要农艺性状的相关关系。【方法】本研究利用部分对三化螟存在抗感差异的重组自交系株系(岗46B×A232,F_9)为材料,对主要农艺性状和幼虫为害性状(枯心指数)及主要农艺性状和成虫产卵量(叶片着卵指数)进行了相关和通径分析。【结果】叶鞘长、播抽天数和倒二叶SPAD值对枯心指数的直接作用为正效应,分蘖数、钩毛和刺毛数及叶片着卵指数对枯心指数直接作用均为负效应,效应大小依次为:播抽天数(0.7852)>倒二叶SPAD值(0.4321)>叶鞘长(0.3986)>分蘖数(-0.3043)>叶片着卵指数(-0.7521)>钩毛和刺毛数(-1.7206);播抽天数和倒二叶SPAD值对叶片着卵指数直接作用为正效应,通过性状间接作用,最终使播抽天数和倒二叶SPAD值两性状与叶片着卵量指数表现为负相关关系;钩毛和刺毛数(400倍镜)对叶片着卵指数的直接作用为最大负效应,通过性状间接作用,叶片腹面上的钩毛和刺毛数最终仍与叶片着卵量性状表现为极显著负相关关系。【结论】本研究结果对水稻种质三化螟抗性评价及抗性品种选育具有指导意义。
【Objective】The relationship among resistance to scirpophaga incertulas and morphological characteristics of rice plants were analyzed.【Method】Using obviously different resistance lines from RILs( Gang46 B × A232,F_(10)),the relationship between main plant morphological characteristics and resistant character to Scirpophaga incertulas were investigated by correlation analysis and path analysis.【Result】To some degree,difference in some plant morphological characteristics also existed in the lines with different resistance. It implied that the resistant character to Scirpophaga incertulas was related to some morphological structure of plant. The correlation analysis and path analysis revealed that leaf sheath length,sow to heading days and top second leaf SPAD value direct contribution to the dead-heart index were positive effect,while No. of tillers,No. of hook hair and prick hair,leaf index of eggs' direct effect exerted negative effect to dead-heart index,all traits by direct contribution to dead-heart index were in order of sow to heading days(0. 7852) > top second leaf SPAD value(0. 4321) > leaf sheath length(0. 3986) > No. of tillers(-0. 3043) > leaf index of eggs(-0. 7521) > No. of hook hair and prick hair(-1. 7206). The results also showed that No. of tillers and top second leaf SPAD value were positive effect contributing to leaf index of eggs directly,by indirect effect of other traits,finally leaf index of eggs had a negative correlation with No. of tillers and top second leaf SPAD value;while No. of hook hair and prick hair had a large negative effect contributing to leaf index of eggs directly,by indirect effect of other traits,and finally leaf index of eggs had a obviously negative correlation with No. Of hook hair and prick hair.【Conclusion】These results could provide some useful information for resistance evaluation of rice germplasm and contribute to breeding rice varieties with resistance to Scirpophaga incertulas.
【Objective】The relationship among resistance to scirpophaga incertulas and morphological characteristics of rice plants were analyzed.【Method】Using obviously different resistance lines from RILs( Gang46 B × A232,F_(10)),the relationship between main plant morphological characteristics and resistant character to Scirpophaga incertulas were investigated by correlation analysis and path analysis.【Result】To some degree,difference in some plant morphological characteristics also existed in the lines with different resistance. It implied that the resistant character to Scirpophaga incertulas was related to some morphological structure of plant. The correlation analysis and path analysis revealed that leaf sheath length,sow to heading days and top second leaf SPAD value direct contribution to the dead-heart index were positive effect,while No. of tillers,No. of hook hair and prick hair,leaf index of eggs' direct effect exerted negative effect to dead-heart index,all traits by direct contribution to dead-heart index were in order of sow to heading days(0. 7852) > top second leaf SPAD value(0. 4321) > leaf sheath length(0. 3986) > No. of tillers(-0. 3043) > leaf index of eggs(-0. 7521) > No. of hook hair and prick hair(-1. 7206). The results also showed that No. of tillers and top second leaf SPAD value were positive effect contributing to leaf index of eggs directly,by indirect effect of other traits,finally leaf index of eggs had a negative correlation with No. of tillers and top second leaf SPAD value;while No. of hook hair and prick hair had a large negative effect contributing to leaf index of eggs directly,by indirect effect of other traits,and finally leaf index of eggs had a obviously negative correlation with No. Of hook hair and prick hair.【Conclusion】These results could provide some useful information for resistance evaluation of rice germplasm and contribute to breeding rice varieties with resistance to Scirpophaga incertulas.
引文
[1]顾正远,肖英方,王益民.水稻品种对二化螟抗性的研究[J].植物保护学报,1989,16(4):245-249.
[2]周祖铭.水稻品种抗二化螟鉴定初步研究[J].植物保护学报,1985,12(3):159-164.
[3]杨丽梅,黄秀清,帅应垣,等.水稻品种小青抗三化螟害的研究[J].中国农业科学,1985,18(5):58-63.
[4]刘光杰,黄和平,谢秀芳,等.早稻品种对二化螟的抗性及基生化基础研究[J].西南农业大学学报,1998,20(5):512-515.
[5]谢戎,李永洪,刘成元,等.籼稻A232及其RILs对三化螟产卵的抗性[J].应用昆虫学报,2015(3):728-735.
[6]李永洪,高方远,谢戎,等.利用重组自交系群体分析籼稻A232抗三化螟相关QTL[J].分子植物育种,2015,13(2):254-260.
[7]李永洪,谢戎,刘成元,等.海南自然诱发条件下籼稻A232抗螟性鉴定[J].西南农业学报,2011,24(6):2178-2180.
[8]腾凯.水稻抗二化螟数量性状基因QTL分析[D].杭州:浙江大学硕士学位论文,2002.
[9]林贤文.水稻抗螟虫及其相关性状的数量遗传分析与验证[D].杭州:浙江大学博士学位论文,2010.
[10]郝丽霞,韩永强,侯茂林,等.辽河流域栽培稻对二化螟的抗性[J].生态学报,2008,28(12):5987-5993.
[11]郝丽霞,韩永强,廖晓兰,等.二化螟与寄主植物间的互作关系[J].湖南农业科学,2009(4):84-87.
[2]周祖铭.水稻品种抗二化螟鉴定初步研究[J].植物保护学报,1985,12(3):159-164.
[3]杨丽梅,黄秀清,帅应垣,等.水稻品种小青抗三化螟害的研究[J].中国农业科学,1985,18(5):58-63.
[4]刘光杰,黄和平,谢秀芳,等.早稻品种对二化螟的抗性及基生化基础研究[J].西南农业大学学报,1998,20(5):512-515.
[5]谢戎,李永洪,刘成元,等.籼稻A232及其RILs对三化螟产卵的抗性[J].应用昆虫学报,2015(3):728-735.
[6]李永洪,高方远,谢戎,等.利用重组自交系群体分析籼稻A232抗三化螟相关QTL[J].分子植物育种,2015,13(2):254-260.
[7]李永洪,谢戎,刘成元,等.海南自然诱发条件下籼稻A232抗螟性鉴定[J].西南农业学报,2011,24(6):2178-2180.
[8]腾凯.水稻抗二化螟数量性状基因QTL分析[D].杭州:浙江大学硕士学位论文,2002.
[9]林贤文.水稻抗螟虫及其相关性状的数量遗传分析与验证[D].杭州:浙江大学博士学位论文,2010.
[10]郝丽霞,韩永强,侯茂林,等.辽河流域栽培稻对二化螟的抗性[J].生态学报,2008,28(12):5987-5993.
[11]郝丽霞,韩永强,廖晓兰,等.二化螟与寄主植物间的互作关系[J].湖南农业科学,2009(4):84-87.