化瘀消癥杀胚中药复方对人输卵管妊娠滋养细胞趋化因子受体CXCR4表达影响
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摘要
目的:探讨化瘀消癓杀胚中药复方对人输卵管妊娠滋养细胞趋化因子受体CXCR4表达的影响。方法:原代培养的输卵管妊娠滋养细胞与低、中、高不同剂量中药复方共同培养48 h后,用免疫细胞化学染色法检测各组细胞中CXCR4表达情况。结果:与对照组比较,中药低、中、高剂量组滋养细胞中CXCR4的相对光密度值分别为79.3%、59.2%、29.3%,表达水平均阳性下降。低剂量组滋养细胞CXCR4的表达水平与阴性对照组比较无统计学差异(P>0.05),中剂量组滋养细胞CXCR4的表达与阴性对照组比较差异有统计学意义(P<0.05),高剂量组滋养细胞CXCR4的表达与阴性对照组比较差异有显著统计学意义(P<0.01)。结论:化瘀消癓杀胚中药复方可显著降低输卵管妊娠滋养细胞中CXCR4的表达。
Objective :To explore the impact of the Huayu Xiaozheng Shapei Decoction on the expression of chemokine receptor CXCR4 in the human tubal gestational trophoblast cell. Methods :Primary cultured tubal gestational trophoblast cells were co-cultured with low,medium and high doses of Chinese herbal compound for 48 hours. The expression of CXCR4 in each group was detected using immunocytochemical staining. Results :Compared with the control group,the relative light density values of CXCR4 were 79.3%,59.2% and 29.3% respectively in the low,medium and high doses group,all showed decreased expression. There was no significant difference on the expression of CXCR4 between the control group and low doses group(P>0.05),and compared with the control group,the difference was statistically significant for the medium doses group(P<0.05)and high dose group(P<0.01). Conclusion :Huayu Xiaozheng Shapei Decoction can significantly decrease the expression of chemokine receptor CXCR4 in the human tubal gestational trophoblast cells.
Objective :To explore the impact of the Huayu Xiaozheng Shapei Decoction on the expression of chemokine receptor CXCR4 in the human tubal gestational trophoblast cell. Methods :Primary cultured tubal gestational trophoblast cells were co-cultured with low,medium and high doses of Chinese herbal compound for 48 hours. The expression of CXCR4 in each group was detected using immunocytochemical staining. Results :Compared with the control group,the relative light density values of CXCR4 were 79.3%,59.2% and 29.3% respectively in the low,medium and high doses group,all showed decreased expression. There was no significant difference on the expression of CXCR4 between the control group and low doses group(P>0.05),and compared with the control group,the difference was statistically significant for the medium doses group(P<0.05)and high dose group(P<0.01). Conclusion :Huayu Xiaozheng Shapei Decoction can significantly decrease the expression of chemokine receptor CXCR4 in the human tubal gestational trophoblast cells.
引文
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[12] REN L,LIU YQ,ZHOU WH,et al. Trophoblast-derived chemokine CX-CL12 promotes CXCR4 expression and invasion of human first-trimester decidual stromal cells[J].Hum Reprod,2012,27:366-374.
[2] Carson DD,Bagchi I,Dey SK,et al. Embryo implantation[J].Develop Biol,2000,223:217-237.
[3] Paria BC,Reese J,Das SK,et al. Deciphering the cross-talk of implantation:advances and challenges[J].Science,2002,296:2185-2188.
[4] Dey SK,Lim H,Das SK,et al. Molecular cues to implantation[J].Endocr Rev,2004,25:341-373.
[5]刘玲. HPLC-MS质控下化瘀消癥杀胚中药复方复方对输卵管妊娠影响的研究[D].广州:广州中医药大学,2012.
[6]袁烁,邓高丕.化瘀消癥杀胚复方含药血清对体外培养输卵管妊娠滋养细胞凋亡的影响[J].中华中医药杂志,2012,27(4):1003-1007.
[7]王瑞雪,邓高丕.化瘀消癥杀胚中药复方复方对体外培养输卵管妊娠滋养细胞凋亡能力的影响[J].中华中医药杂志,2014,29(5):1645-1649.
[8]刘玲,袁烁,邓高丕.化瘀消癥杀胚中药复方复方给药血浆的药物成分检测[J].时珍国医国药,2014,25(7):1759-1761.
[9] Dominguez F,Yanez-Mo M,Sanchez-Madrid F,et al. Embryonic implantation and leukocyte transendothelial migration:different processes with similar players?[J].FASEB J,2005,19(9):1056-1060.
[10] WU X,LI DJ,YUAN MM,et al. The expression of CXCR4/CXCL12 in first-trimester human trophoblast cells[J].Biol Reprod,2004,70(6):1877-1885.
[11] ZHOU WH,DU MR,DONG L,et al. Chemokine CXCL12promotes thecross-talk between trophoblasts and decidual stromal cells in human first-trimester pregnancy[J].Hum Reprod,2008,23:2669-2679.
[12] REN L,LIU YQ,ZHOU WH,et al. Trophoblast-derived chemokine CX-CL12 promotes CXCR4 expression and invasion of human first-trimester decidual stromal cells[J].Hum Reprod,2012,27:366-374.