α-单月桂酸甘油酯对致病菌的抑制作用
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摘要
试验旨在通过牛津杯抑菌实验和摇瓶实验探究α-单月桂酸甘油酯对大肠杆菌、沙门氏菌和金黄色葡萄球菌的抑菌效果。结果显示,浓度≤40 mg·mL~(-1)的α-单月桂酸甘油酯对大肠杆菌无明显抑菌作用,浓度≥0.156 mg·mL~(-1)的α-单月桂酸甘油酯对沙门氏菌有抑制作用,浓度≥0.078 mg·mL~(-1)的α-单月桂酸甘油酯对金黄色葡萄球菌有抑制作用。浓度为1 mg·mL~(-1)的α-单月桂酸甘油酯对金黄色葡萄球菌的抑制作用在培养4 h出现,对沙门氏菌的抑制作用在培养12 h出现。
This experiment aims to investigate the antibacterial effect of α-glycerol monolaurate on Escherichia coli, Salmonella and Staphylococcus aureus. The results show that: α-glycerol monolaurate with concentration ≤40 mg·mL~(-1) had no significant antibacterial effect on Escherichia coli, and α-glycerol monolaurate at a concentration of ≥0.156 mg·mL~(-1) inhibited Salmonella. α-glycerol monolaurate with a concentration of ≥0.078 mg·mL~(-1) inhibited Staphylococcus aureus. The inhibitory effect of α-glycerol monolaurate at a concentration of 1 mg·mL~(-1) on Staphylococcus aureus appeared four hours after culture, and the inhibitory effect on Salmonella occurred after 12 hours of culture.
This experiment aims to investigate the antibacterial effect of α-glycerol monolaurate on Escherichia coli, Salmonella and Staphylococcus aureus. The results show that: α-glycerol monolaurate with concentration ≤40 mg·mL~(-1) had no significant antibacterial effect on Escherichia coli, and α-glycerol monolaurate at a concentration of ≥0.156 mg·mL~(-1) inhibited Salmonella. α-glycerol monolaurate with a concentration of ≥0.078 mg·mL~(-1) inhibited Staphylococcus aureus. The inhibitory effect of α-glycerol monolaurate at a concentration of 1 mg·mL~(-1) on Staphylococcus aureus appeared four hours after culture, and the inhibitory effect on Salmonella occurred after 12 hours of culture.
引文
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[10]刘梦芸,王建莉,冯凤琴.饲粮添加单月桂酸甘油酯对蛋鸡生产性能、蛋品质、血清生化指标、免疫器官指数和腹脂形态的影响[J].中国家禽, 2017, 39(17):24-30.
[11]王唯霖.中草药提取物的抑菌机理及对大肠杆菌的耐药性研究[D].曲阜:曲阜师范大学, 2015.
[12] Bunkova L, Krejc J, Janis R, et al. Influence of monoacylglycerols on growth inhibition of micromycetes in vitro and on bread[J].European Journal of Lipid Science and Technology, 2010, 112(2):173-179.
[13] Desbois A P, Smith V J. Antibacterial free fatty acids:activities,mechanisms of action and biotechnological potential[J]. Applied Microbiology&Biotechnology, 2010, 85(6):1 629-1 642.
[14]蒋增良,张辉,杜鹃,等.月桂酸单甘油酯抑菌机理、影响因素及其复配体系的抑菌特性[J].中国食品学报, 2016, 16(3):146-151.
[15] Peterson M L, Schlievert P M. Glycerol monolaurate inhibits the effects of gram-positive select agents on eukaryotic cells[J]. Biochemistry, 2006, 45(7):2 387-2 397.
[2]张璐,张雪伟,袁宗辉,等.畜禽沙门氏菌病防制研究进展[J].中国畜牧兽医, 2017, 44(7):2 103-2 111.
[3]杜宗沛,黄银云.动物传染病[M].北京:中国林业出版社, 2012.
[4]林杰,陆俊贤,龚建森.规模化鸡场金黄色葡萄球菌的分离鉴定及药敏试验[J].中国家禽, 2011, 33(2):61.
[5]王晓,俞英.中国北方奶牛金葡菌乳房炎感染现状及耐药性和流行类型研究进展[J].畜牧兽医学报, 2015(9):1 477-1 488.
[6]蒋增良,杨明,杜鹃,等.月桂酸单甘油酯抑菌抗病毒特性及其在食品中的应用[J].中国粮油学报, 2015, 30(2):142-146.
[7]冯凤琴,杜鹃,王小刘.食品防腐乳化剂月桂酸单甘油酯及其在食品中的应用[J].中国食品添加剂, 2009(S1):173-177.
[8]张希,阙斐,宗红,等.月桂酸单甘油酯及其复配物对食品腐败菌的抑菌特性[J].中国食品学报, 2016(5):89-94.
[9]李涛,王春维,万志友,等.椰子油和单月桂酸甘油酯对断奶仔猪生产性能及粪便微生物的影响[J].粮食与饲料工业, 2013(6):54-57.
[10]刘梦芸,王建莉,冯凤琴.饲粮添加单月桂酸甘油酯对蛋鸡生产性能、蛋品质、血清生化指标、免疫器官指数和腹脂形态的影响[J].中国家禽, 2017, 39(17):24-30.
[11]王唯霖.中草药提取物的抑菌机理及对大肠杆菌的耐药性研究[D].曲阜:曲阜师范大学, 2015.
[12] Bunkova L, Krejc J, Janis R, et al. Influence of monoacylglycerols on growth inhibition of micromycetes in vitro and on bread[J].European Journal of Lipid Science and Technology, 2010, 112(2):173-179.
[13] Desbois A P, Smith V J. Antibacterial free fatty acids:activities,mechanisms of action and biotechnological potential[J]. Applied Microbiology&Biotechnology, 2010, 85(6):1 629-1 642.
[14]蒋增良,张辉,杜鹃,等.月桂酸单甘油酯抑菌机理、影响因素及其复配体系的抑菌特性[J].中国食品学报, 2016, 16(3):146-151.
[15] Peterson M L, Schlievert P M. Glycerol monolaurate inhibits the effects of gram-positive select agents on eukaryotic cells[J]. Biochemistry, 2006, 45(7):2 387-2 397.