基于中医方证代谢组学的中药质量标志物发现研究
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摘要
中药质量是影响中医药传承和发展的重要因素,然而由于中药成分的复杂性和复方应用的特殊性,现今的中药质量标准存在难以保障临床疗效的问题。刘昌孝院士提出中药质量标志物的概念和基本属性,为中药质量标准研究指明了方向。中医方证代谢组学是整合了中药血清药物化学与代谢组学技术,在方证对应并显效的情况下,发现与临床疗效相关、体现方剂配伍、可追溯体内代谢和制备过程的中药药效物质基础的研究策略,是发现中药质量标志物的有效方法。利用中医方证代谢组学方法已经对茵陈蒿汤、六味地黄丸等方剂及男仕胶囊、AS1350等中药保健品质量标志物的发现研究进行了探索。
The quality of Chinese materia medica(CMM) has become an important factor affecting the inheritance and development of traditional Chinese medicine. However, due to the complexity of CMM ingredients and the specificity of prescription application, the current quality standards of CMM are difficult to guarantee clinical efficacy. Academician Changxiao Liu proposed the concept of the quality marker(Q-marker) of CMM and its basic attributes, which provided a direction for the study of CMM quality standards. Chinmedomics, integrating serum pharmacochemistry and metabolomics technology, under the conditions of correspondence of prescription-syndrome with therapeutic effect, can be used to find the Q-markers of CMM which are related to the clinical efficacy, and reflect the compatibility of prescriptions, and trace the in vivo metabolism and preparation process. The research strategy based on Chinmedomics is an effective method for discovering the Q-markers of CMM, it has been applied to discovery Q-markers of Yinchenhao Decoction, Liuwei Dihuang Pill, Nan Shi Capsule, AS1350, and other CMM formula as well as the health products.
The quality of Chinese materia medica(CMM) has become an important factor affecting the inheritance and development of traditional Chinese medicine. However, due to the complexity of CMM ingredients and the specificity of prescription application, the current quality standards of CMM are difficult to guarantee clinical efficacy. Academician Changxiao Liu proposed the concept of the quality marker(Q-marker) of CMM and its basic attributes, which provided a direction for the study of CMM quality standards. Chinmedomics, integrating serum pharmacochemistry and metabolomics technology, under the conditions of correspondence of prescription-syndrome with therapeutic effect, can be used to find the Q-markers of CMM which are related to the clinical efficacy, and reflect the compatibility of prescriptions, and trace the in vivo metabolism and preparation process. The research strategy based on Chinmedomics is an effective method for discovering the Q-markers of CMM, it has been applied to discovery Q-markers of Yinchenhao Decoction, Liuwei Dihuang Pill, Nan Shi Capsule, AS1350, and other CMM formula as well as the health products.
引文
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[22]李先娜,邹迪,李冰冰,等.基于体内过程的关黄柏在知柏地黄丸等类方配伍环境下血中移行成分分析[J].世界科学技术—中医药现代化,2016,18(10):1730-1739.
[23]Li X N,Zhang A,Wang M,et al.Screening the active compounds of Phellodendri Amurensis Cortex for treating prostate cancer by high-throughput chinmedomics[J].Sci Rep,2017,doi:10.1038/srep46234.
[24]张天雷,邱时,李先娜,等.基于细胞代谢组学技术的关黄柏潜在有效成分木兰碱对前列腺癌细胞的治疗作用研究[J].世界科学技术—中医药现代化,2016,18(10):1740-1748.
[25]Li X,Zhang A,Sun H,et al.Metabolic characterization and pathway analysis of berberine protects against prostate cancer[J].Oncotarget,2017,8(39):65022-65041.
[26]Wang L,Yan G,Zhang A,et al.Fingerprinting and simultaneous determination of alkaloids and limonins in Phellodendri Amurensis Cortex from different locations by high-performance liquid chromatography with diode array detection[J].J Chromatogr Sci,2015,53(1):161-166.
[2]刘昌孝.从中药资源-质量-质量标志物认识中药产业的健康发展[J].中草药,2016,47(18):3149-3154.
[3]Liu C X,Cheng Y Y,Guo D A,et al.A new concept on quality marker for quality assessment and process control of chinese medicines[J].Chin Herb Med,2017,9(1):3-13.
[4]张铁军,白钢,陈常青,等.中药质量标志物——基于“五原则”的复方中药质量标志物(Q-marker)研究路径[J].中草药,2018,49(1):1-13.
[5]张铁军,王杰,陈常青,等.基于中药属性和作用特点的中药质量标志物研究与质量评价途径[J].中草药,2017,48(6):1051-1060.
[6]王喜军.中药药效物质基础研究的系统方法学——中医方证代谢组学[J].中国中药杂志,2015,40(1):13-17.
[7]Wang X J,Zhang A,Sun H,et al.Discovery and development of innovative drug from traditional medicine by integrated chinmedomics strategies in the post-genomic era[J].Tr AC Trends Anal Chem,2016,doi:10.1016/j.trac.2015.11.010.
[8]Wang X J,Zhang A,Yan G,et al.UHPLC-MS for the analytical characterization of traditional Chinese medicines[J].Tr AC Trends Anal Chem,2014,63:180-187.
[9]Wang X J.Chinmedomics[M].San Diego:Academic Press,Elsevier,2015.
[10]Wang X J.Serum Pharmacochemistry of Traditional Chinese Medicine[M].San Diego:Academic Press,Elsevier,2017.
[11]Wang X,Zhang A,Zhou X,et al.An integrated chinmedomics strategy for discovery of effective constituents from traditional herbal medicine[J].Sci Rep,2016,6:18997.
[12]Zhang A,Sun H,Wang X J.Mss spectrometry-driven drug discovery for development of herbal medicine[J].Mass Spectrom Rev,2018,37(3):307-320.
[13]Wang X,Zhang A,Sun H.Future perspectives of Chinese medical formulae:Chinmedomics as an effector[J].OMICS,2012,16(7/8):414-421.
[14]Liu C X.Chinmedomics builds a bridge from traditional to modern research of traditional Chinese medicine[J].Chin Herb Med,2016,8(4):297-298.
[15]Wang X,Zhang A,Wang P,et al.Metabolomics coupled with proteomics advancing drug discovery toward more agile development of targeted combination therapies[J].Mol Cell Proteomics,2013,12(5):1226-1238.
[16]Wang X,Sun W,Sun H,et al.Analysis of the constituents in the rat plasma after oral administration of Yin Chen Hao Tang by UPLC/Q-TOF-MS/MS[J].J Pharm BiomedAnal,2008,46(3):477-490.
[17]张爱华,孙晖,闫广利,等.中医方证代谢组学——中医药研究的新策略[J].中国中药杂志,2015,40(4):569-576.
[18]Wang X,Lv H,Sun H,et al.Development of a rapid and validated method for investigating the metabolism of scoparone in rat using ultra-performance liquid chromatography/electrospray ionization quadruple timeof-flight mass spectrometry[J].Rapid Commun Mass Spectrom,2007,21(23):3883-3890.
[19]李秋菊,王萍,王美佳,等.基于中医方证代谢组学技术的六味地黄丸干预脑瘫大鼠模型研究[J].世界科学技术—中医药现代化,2016,18(10):1684-1696.
[20]刘琦,赵宏伟,张爱华,等.基于中医方证代谢组学研究男仕胶囊治疗肾阳虚证的药效物质基础及作用机制[J].中国中药杂志,2016,41(15):2901-2914.
[21]Liu Q,Zhang A,Wang L,et al.High-throughput chinmedomics-based prediction of effective components and targets from herbal medicine AS1350[J].Sci Rep,2016,doi:10.1038/srep38437.
[22]李先娜,邹迪,李冰冰,等.基于体内过程的关黄柏在知柏地黄丸等类方配伍环境下血中移行成分分析[J].世界科学技术—中医药现代化,2016,18(10):1730-1739.
[23]Li X N,Zhang A,Wang M,et al.Screening the active compounds of Phellodendri Amurensis Cortex for treating prostate cancer by high-throughput chinmedomics[J].Sci Rep,2017,doi:10.1038/srep46234.
[24]张天雷,邱时,李先娜,等.基于细胞代谢组学技术的关黄柏潜在有效成分木兰碱对前列腺癌细胞的治疗作用研究[J].世界科学技术—中医药现代化,2016,18(10):1740-1748.
[25]Li X,Zhang A,Sun H,et al.Metabolic characterization and pathway analysis of berberine protects against prostate cancer[J].Oncotarget,2017,8(39):65022-65041.
[26]Wang L,Yan G,Zhang A,et al.Fingerprinting and simultaneous determination of alkaloids and limonins in Phellodendri Amurensis Cortex from different locations by high-performance liquid chromatography with diode array detection[J].J Chromatogr Sci,2015,53(1):161-166.