基于氮掺杂多孔碳及PoPD电化学免疫传感器的构建
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摘要
氮掺杂多孔碳具有良好的生物相容性,聚邻苯二胺具有丰富的氨基以及亚氨基,能提高电化学免疫传感器的性能。本文构建了一种具有较高选择性和稳定性以及灵敏性的无标记型电化学免疫传感器用于检测甲胎蛋白(AFP)。基于氮参杂多孔碳/聚邻苯二胺修饰电极表面,采用循环伏安(CV)及交流阻抗法(EIS)研究了修饰电极表面的电化学特性。在优化的条件下,该电化学免疫传感器的相应电流与AFP的浓度在0.005-100 ng/mL范围内呈现良好的线性关系,检测限为0.0006 ng/mL。新型电化学免疫传感器可为临床应用提供潜在的应用。
In this study, we constructed a label-free electrochemical immunosensor for highly selective detection, stability and sensitive detection of alpha-fetoprotein(AFP) based on PoPD and N-doped porous carbon. The electrochemical characteristics with the modified electrode surface were studied by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS)measurements. The using of N-doped porous carbon greatly enhanced the biology catalytic activity due to its large specific surface and excellent biocompatibility. PoPD has large amino which can improved its ability to fixed anti-AFP. Under the optimized conditions, the results show that the immunosensor have excellent electrochemical properties with a wide concentration range from 0.005 to 100 ng/mL and a detection limit of 0.0006 ng/mL. The new type of electrochemical immunosensor may provide potential applications for the clinic application.
In this study, we constructed a label-free electrochemical immunosensor for highly selective detection, stability and sensitive detection of alpha-fetoprotein(AFP) based on PoPD and N-doped porous carbon. The electrochemical characteristics with the modified electrode surface were studied by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS)measurements. The using of N-doped porous carbon greatly enhanced the biology catalytic activity due to its large specific surface and excellent biocompatibility. PoPD has large amino which can improved its ability to fixed anti-AFP. Under the optimized conditions, the results show that the immunosensor have excellent electrochemical properties with a wide concentration range from 0.005 to 100 ng/mL and a detection limit of 0.0006 ng/mL. The new type of electrochemical immunosensor may provide potential applications for the clinic application.
引文
[1]Wang H,He L,Zhang Y,et al.Label-free immunosensor based on Pd nanoplates for amperometric immunoassay of alpha-fetoprotein[J].Biosensors&Bioelectronics,2013,53C(6):305-309.
[2]Zhou H,Gan N,Li T,et al.The sandwich-type electrochemiluminescence immunosensor forα-fetoprotein based on enrichment by Fe3O4-Au magnetic nano probes and signal amplification by Cd S-Au composite nanoparticles labeled anti-AFP[J].Analytica chimica acta,2012,746:107-113.
[3]王知.甲胎蛋白生物传感器的制备和研究[D].苏州:苏州大学,2009.
[4]And E C,C J C.A Novel Self-Assembled Nanoparticulate Film for Covalent Attachment of Antibodies to Plastic[J].Langmuir,2003,19(10):4509-4511.
[5]Lin J,Ju H.Electrochemical and chemiluminescent immunosensors for tumor markers[J].Biosensors&Bioelectronics,2005,20(8):1461-1470.
[6]Bahadr E B,Sezgintürk M K.Applications of electrochemical immunosensors for early clinical diagnostics[J].Talanta,2015,132:162-174.
[7]Loomans E E M G,Roelen A J M,Van Damme H S,et al.Assessment of the functional affinity constant of monoclonal antibodies using an improved enzyme-linked immunosorbent assay[J].Journal of Immunological Methods,1995,184(2):207-217.
[8]Li Q,Zeng L,Wang J,et al.Magnetic Mesoporous Organic Inorganic Ni Co2O4Hybrid Nanomaterials for Electrochemical Immunosensors[J].Acs Appl mater interfaces,2011,3(4):1366-1373.
[9]Kroto H W,Heath J R,O'Brien S C,et al.C60:Buckminsterfullerene[J].Nature,1985,318(6042):162-163.
[10]Orikasa H,Akahane T,Okada M,Tong Y,Ozaki J-i,Kyotani T.Electrochemical Behavior of Carbon Nanorod Arrays Having Different Graphene Orientations and Crystallinity[J].J Mater Chem,2009,19(26):4615-4621.
[11]Sun X,Li Y.Colloidal Carbon Spheres and Their Core/Shell Structures with Noble-Metal Nanoparticles[J].Angew Chem Int Ed,2004,43:597-601.
[12]黄维垣.高技术有机高分子材料进展[M].北京:化学工业出版社,1994.
[13]Qiu J D,Huang H,Liang R P.Biocompatible and label-free amperometric immunosensor for hepatitis B surface antigen using a sensing film composed of poly(allylamine)-branched ferrocene and gold nanoparticles[J].Microchimica Acta,2011,174(1-2):97-105.
[14]Xiao S,Zheng C,Liang L,et al.One-Pot Synthesis and Catalyst Support Application of Mesoporous N-Doped Carbonaceous Materias[J].J Mater Chem,2012,22(24):12149-12154.
[15]Zhen Y L,Guo X Z,Zhi Y L,et al.One-Step Scalable Preparation of N-doped Nanoporous Carbon as High-Performance Electrocatalysts for Oxygen Reduction Reaction[J].Nano Res,2013,6(4):293-301.
[16]Ghalkhani M,Shahrokhian S.Application of carbon nanoparticle/chitosan modified electrode for the square-wave adsorptive anodic striping voltammetric determination of Niclosamide[J].Electrochemistry Communications,2010,12(1):66-69.
[2]Zhou H,Gan N,Li T,et al.The sandwich-type electrochemiluminescence immunosensor forα-fetoprotein based on enrichment by Fe3O4-Au magnetic nano probes and signal amplification by Cd S-Au composite nanoparticles labeled anti-AFP[J].Analytica chimica acta,2012,746:107-113.
[3]王知.甲胎蛋白生物传感器的制备和研究[D].苏州:苏州大学,2009.
[4]And E C,C J C.A Novel Self-Assembled Nanoparticulate Film for Covalent Attachment of Antibodies to Plastic[J].Langmuir,2003,19(10):4509-4511.
[5]Lin J,Ju H.Electrochemical and chemiluminescent immunosensors for tumor markers[J].Biosensors&Bioelectronics,2005,20(8):1461-1470.
[6]Bahadr E B,Sezgintürk M K.Applications of electrochemical immunosensors for early clinical diagnostics[J].Talanta,2015,132:162-174.
[7]Loomans E E M G,Roelen A J M,Van Damme H S,et al.Assessment of the functional affinity constant of monoclonal antibodies using an improved enzyme-linked immunosorbent assay[J].Journal of Immunological Methods,1995,184(2):207-217.
[8]Li Q,Zeng L,Wang J,et al.Magnetic Mesoporous Organic Inorganic Ni Co2O4Hybrid Nanomaterials for Electrochemical Immunosensors[J].Acs Appl mater interfaces,2011,3(4):1366-1373.
[9]Kroto H W,Heath J R,O'Brien S C,et al.C60:Buckminsterfullerene[J].Nature,1985,318(6042):162-163.
[10]Orikasa H,Akahane T,Okada M,Tong Y,Ozaki J-i,Kyotani T.Electrochemical Behavior of Carbon Nanorod Arrays Having Different Graphene Orientations and Crystallinity[J].J Mater Chem,2009,19(26):4615-4621.
[11]Sun X,Li Y.Colloidal Carbon Spheres and Their Core/Shell Structures with Noble-Metal Nanoparticles[J].Angew Chem Int Ed,2004,43:597-601.
[12]黄维垣.高技术有机高分子材料进展[M].北京:化学工业出版社,1994.
[13]Qiu J D,Huang H,Liang R P.Biocompatible and label-free amperometric immunosensor for hepatitis B surface antigen using a sensing film composed of poly(allylamine)-branched ferrocene and gold nanoparticles[J].Microchimica Acta,2011,174(1-2):97-105.
[14]Xiao S,Zheng C,Liang L,et al.One-Pot Synthesis and Catalyst Support Application of Mesoporous N-Doped Carbonaceous Materias[J].J Mater Chem,2012,22(24):12149-12154.
[15]Zhen Y L,Guo X Z,Zhi Y L,et al.One-Step Scalable Preparation of N-doped Nanoporous Carbon as High-Performance Electrocatalysts for Oxygen Reduction Reaction[J].Nano Res,2013,6(4):293-301.
[16]Ghalkhani M,Shahrokhian S.Application of carbon nanoparticle/chitosan modified electrode for the square-wave adsorptive anodic striping voltammetric determination of Niclosamide[J].Electrochemistry Communications,2010,12(1):66-69.