乙醛脱氢酶1和Numb在食管鳞癌患者中的表达及意义
摘要
目的探讨乙醛脱氢酶(ALDH) 1和Numb基因在食管鳞癌患者中的表达及意义。方法应用实时荧光定量PCR技术检测120例食管鳞癌及癌旁组织标本中ALDH1和Numb基因的表达情况,并且采用Western印迹检测ALDH1和Numb蛋白表达,探讨其与患者临床特征的关系。结果食管鳞癌组织中ALDH1 mRNA相对表达量(11. 07±1. 18)显著高于癌旁组织(7. 13±2. 05,P<0. 05);而食管鳞癌组织中Numb mRNA的相对表达量(2. 02±0. 02)显著低于癌旁组织(5. 87±0. 47,P<0. 05)。ALDH1蛋白和Numb蛋白表达与转移情况、浸润深度、最大直径、分化程度及Ki67指数密切相关(P<0. 05)。AL-DH1蛋白和Numb蛋白表达呈负相关(r=-0. 46,P=0. 034 9)。结论 ALDH1在食管癌的生物学过程中发挥致癌作用,Numb基因发挥抑癌的作用。
引文
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8 巩佃霞,李贵新,路中,等. ALDH1在胃癌组织中的表达及临床意义[J].澭坊医学院学报,2014; 36(1):36-8.
9 覃宇奇,覃冠平,蓝川,等.乙醛脱氢酶1、CD133在鼻咽癌组织中的表达及临床意义[J].疑难病杂志,2015; 14(6):590-2.
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12 王超,张楚瑶,冯炜炜. Numb在肿瘤中的作用[J].国际肿瘤学杂志,2012; 39(7):541-4.
13 陈小君,叶枫,陈怀增,等.细胞分裂方向的改变和Numb的表达增高在宫颈鳞癌中的作用[J].实用癌症杂志,2005; 20(5):455-7.
2 王孟,郝长青,赵德利,等. 2005-2009年中国食管癌高发区河南省林州市、山东省肥城市食管癌及癌前病变人群分布研究[J].中华预防医学杂志,2015; 49(8):677-82.
3 Shimadah H,Shiratori T,Okazumi S,et al. Have surgical outcomes of pathologic T4 esophageal squamous cell carcinoma really improved?Analysis of 268 cases during 45 years of experience[J]. Am Coll Surg,2008; 206(1):48-56.
4 Chen YC,Chen YW,Hsu HS,et al. Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer[J].Biochem Biophys Res Commun,2009; 385(3):307-3013.
5 Colaluca IN,Tosoni D,Nuciforo P,et al. NUMB controls p53 tumour suppressor activity[J].Nature,2008; 451(7174):76-80.
6 Chute JP,Muramoto GG,Whitesides J,et al.Inhibition of aldehyde dehydrogenase and retinoid signaling induces the expansion of human hematopoietic stem cells[J]. Proc Natl Acad Sci U S A,2006; 103(31):11707-12.
7 Ginestier C,Hur MH,Charafe-Jauffret E,et al.ALDH1 is a marker of normal and ma-lignant human mammary stem cells and a predictor of poor clinical outcome[J].Cell Stem Cell,2007; 1(5):555-67.
8 巩佃霞,李贵新,路中,等. ALDH1在胃癌组织中的表达及临床意义[J].澭坊医学院学报,2014; 36(1):36-8.
9 覃宇奇,覃冠平,蓝川,等.乙醛脱氢酶1、CD133在鼻咽癌组织中的表达及临床意义[J].疑难病杂志,2015; 14(6):590-2.
10 Yabuuchi S,Pai SG,Campbell NR,et al. Notch signaling pathway targeted therapy suppresses tumor progression and metastatic spread in pancreatic cancer[J].Cancer Lett,2013; 335(1):41-51.
11 Artavanis-Tsakonsa S,Rand MD,Lake RJ. Notch signaling:cell fate control and signal integration in developmen[tJ].Science,1999; 284(5415):770-6.
12 王超,张楚瑶,冯炜炜. Numb在肿瘤中的作用[J].国际肿瘤学杂志,2012; 39(7):541-4.
13 陈小君,叶枫,陈怀增,等.细胞分裂方向的改变和Numb的表达增高在宫颈鳞癌中的作用[J].实用癌症杂志,2005; 20(5):455-7.