维药阿那其根提取物对过氧化氢致PC12细胞损伤的保护研究
|
摘要
目的研究维药阿那其根提取物对过氧化氢诱导细胞凋亡的影响。方法培养PC12细胞,建立过氧化氢导致细胞损伤的实验模型,将细胞随机分为正常对照组、模型组、阿那其根水提物组、阿那其根醇提物组、阳性对照组。通过CCK-8法检测细胞增殖以及不同加药组细胞的活力。用Hochest33342染料观看细胞形态改变,(PE)染色流式细胞术测出各组细胞凋亡率。结果与模型组比较,不同浓度的阿那齐根提取物组对细胞活力有明显的增殖作用,差异有统计学意义(P<0.05)。Hochest33342染色结果显示不同浓度的加药组细胞的形态明显区别于模型组,尤其是高剂量组细胞形态与数量接近于正常组细胞。流式细胞术证明模型组细胞早期凋亡率高于正常对照组,不同浓度给药组细胞的早期凋亡率低于模型组。结论阿那其根提取物可增加细胞存活率,并抑止细胞凋亡率升高。
Objective To investigate the effects of Anacyclus pyrethrum extracts on Hydrogen peroxide-induced apoptosis in PC12 cells. Methods PC12 cells were divided into groups and measured cell viability by CCK-8 cell proliferation assay and were also characterized the degree of PC12 cell apoptosis induced by H2O2 stained with Hoechst 33342 and PE Annexin V. Results Compared with the model group,the value of cell proliferation of different amounts of Anacyclus pyrethrum extract had statistically significant( P < 0. 05). Hochest 33342 staining found that cell morphology and number of cells that were treated with different concentrations of extract were close to the normal group and different from model group. Annexin-V / PE double staining showed that apoptosis rate of the model group was higher than the normal group and the early apoptosis rate of the cells in different concentration extract was less than the model group. Conclusion Anacyclus Pyrethrum clearly reduces percentage of apoptosis PC12 cells induced by hydrogen peroxide.
Objective To investigate the effects of Anacyclus pyrethrum extracts on Hydrogen peroxide-induced apoptosis in PC12 cells. Methods PC12 cells were divided into groups and measured cell viability by CCK-8 cell proliferation assay and were also characterized the degree of PC12 cell apoptosis induced by H2O2 stained with Hoechst 33342 and PE Annexin V. Results Compared with the model group,the value of cell proliferation of different amounts of Anacyclus pyrethrum extract had statistically significant( P < 0. 05). Hochest 33342 staining found that cell morphology and number of cells that were treated with different concentrations of extract were close to the normal group and different from model group. Annexin-V / PE double staining showed that apoptosis rate of the model group was higher than the normal group and the early apoptosis rate of the cells in different concentration extract was less than the model group. Conclusion Anacyclus Pyrethrum clearly reduces percentage of apoptosis PC12 cells induced by hydrogen peroxide.
引文
[1]易沙克江·马合穆德.中国医学百科全说.维吾尔医学[M].上海:上海科学技术出版社,2005:9.
[2]高琳琳,李福荣,康莉,等.蚤休醇提物对H2O2损伤的ECV304细胞的细胞周期与凋亡的影响[J].中国药理学通报,2008,24(11):1513-1517.
[3]陈瑾歆,陈建业.自由基与衰老关系的研究进展[J].川北医学院学报,2004,19(1):207-209.
[4]热孜亚·艾买提,麦合苏木·艾克木,毕晓娟,等.维药异常黑胆质成熟剂多酚类提取物对神经细胞氧化损伤的预防作用[J].新疆医科大学报,2015,38(5):543-547.
[5]买迪娜·吐尔逊,热娜古丽·艾则孜,努尔买买提·艾买提,等.葡萄糖代谢异常与阿尔茨海默病发病机制的研究进展[J].中华老年心脑血管病杂志,2014,16(11):1224-1226.
[6]张筱,杨泽.阿尔茨海默病遗传学研究进展[J].中国老年保健医学,2015,13(1):19-23.
[7]热孜亚·艾买提,祖丽菲亚·吾斯曼,努尔买买提·艾买提.天然多酚类抗氧化成分及治疗阿尔茨海默病的潜力[J].医学综述,2015,21(9):1537-1540.
[8]安丽荣,郭艳芹,金连弘,等.丙酮酸对过氧化氢诱发PC12细胞损伤的保护作用[J].中国老年学杂志,2009,29(13):1615-1617.
[9]刘晓峰,吴迪,吴岩,等.干细胞治疗阿尔茨海默病的现状及未来[J].中国组织工程研究,2013,17(40):7132-7137.
[10]Karch CM,Goate AM.Alzheimer’s disease risk genes and mechanisms of disease pathogenesis[J].Biological Psychiatry,2015,77(1):43-51.
[11]唐红梅,白雪.阿尔茨海默病发病机制及治疗研究进展[J].2010,33(12):1455-1457.
[12]马英,曹云鹏.阿尔茨海默病的神经免疫炎症发病机制及其治疗研究进展[J].中国老年学杂志,2010,30(21):3200-3204.
[13]朱贝贝,刘萍萍,李淑玲,等.当归红芪超滤膜提取物对过氧化氢致PC12细胞凋亡的影响[J].中国中医药信息杂志,2016,23(6):68-72.
[14]塔依尔·吐尔松,买吾拉尼江·依孜布拉,买地尼也提·尼亚孜,等.异常黏液质成熟剂颗粒对APP/PS1转基因小鼠行为学和海马组织病理形态学的影响[J].中国中医药信息杂志,2016,23(4):41-44.
[15]文金隆.天麻酚性成分对H2O2诱导PC12细胞氧化损伤的保护作用研究[D].昆明:云南中医学院,2013.
[2]高琳琳,李福荣,康莉,等.蚤休醇提物对H2O2损伤的ECV304细胞的细胞周期与凋亡的影响[J].中国药理学通报,2008,24(11):1513-1517.
[3]陈瑾歆,陈建业.自由基与衰老关系的研究进展[J].川北医学院学报,2004,19(1):207-209.
[4]热孜亚·艾买提,麦合苏木·艾克木,毕晓娟,等.维药异常黑胆质成熟剂多酚类提取物对神经细胞氧化损伤的预防作用[J].新疆医科大学报,2015,38(5):543-547.
[5]买迪娜·吐尔逊,热娜古丽·艾则孜,努尔买买提·艾买提,等.葡萄糖代谢异常与阿尔茨海默病发病机制的研究进展[J].中华老年心脑血管病杂志,2014,16(11):1224-1226.
[6]张筱,杨泽.阿尔茨海默病遗传学研究进展[J].中国老年保健医学,2015,13(1):19-23.
[7]热孜亚·艾买提,祖丽菲亚·吾斯曼,努尔买买提·艾买提.天然多酚类抗氧化成分及治疗阿尔茨海默病的潜力[J].医学综述,2015,21(9):1537-1540.
[8]安丽荣,郭艳芹,金连弘,等.丙酮酸对过氧化氢诱发PC12细胞损伤的保护作用[J].中国老年学杂志,2009,29(13):1615-1617.
[9]刘晓峰,吴迪,吴岩,等.干细胞治疗阿尔茨海默病的现状及未来[J].中国组织工程研究,2013,17(40):7132-7137.
[10]Karch CM,Goate AM.Alzheimer’s disease risk genes and mechanisms of disease pathogenesis[J].Biological Psychiatry,2015,77(1):43-51.
[11]唐红梅,白雪.阿尔茨海默病发病机制及治疗研究进展[J].2010,33(12):1455-1457.
[12]马英,曹云鹏.阿尔茨海默病的神经免疫炎症发病机制及其治疗研究进展[J].中国老年学杂志,2010,30(21):3200-3204.
[13]朱贝贝,刘萍萍,李淑玲,等.当归红芪超滤膜提取物对过氧化氢致PC12细胞凋亡的影响[J].中国中医药信息杂志,2016,23(6):68-72.
[14]塔依尔·吐尔松,买吾拉尼江·依孜布拉,买地尼也提·尼亚孜,等.异常黏液质成熟剂颗粒对APP/PS1转基因小鼠行为学和海马组织病理形态学的影响[J].中国中医药信息杂志,2016,23(4):41-44.
[15]文金隆.天麻酚性成分对H2O2诱导PC12细胞氧化损伤的保护作用研究[D].昆明:云南中医学院,2013.