紫叶李嫩枝扦插生根过程中生理生化指标分析
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摘要
对紫叶李(Prunus cerasifera)嫩枝扦插生根影响因素:外源调节物质种类(IBA、NAA、6-BA)、外源调节物质浓度(100、200、400mg/L)、外源调节物质作用时间(0.5、1.0、2.0h)进行了正交试验分析,测定了插穗韧皮部在生根过程中的可溶性糖、可溶性淀粉、可溶性蛋白、生长素(IAA)、脱落酸(ABA)、赤酶素(GA3)含量及过氧化物酶(POD)、多酚氧化酶(PPO)酶活性的变化。结果表明,处理后扦插第27天时,可溶性糖含量、过氧化物酶(POD)和多酚氧化酶(PPO)酶活性以及内源GA3与IAA含量由高到低的处理依次为IBA>NAA>6-BA,插穗生根率高低变化也有类似的规律。内源ABA含量的变化与其他两种激素相反,且对紫叶李插穗生根具有抑制作用。
This project aims to analyze the influencing factors on Prunus cerasiferatwig cutting and rooting:types of exogenous regulation substances(IBA,NAA,6-BA),concentration of exogenous regulation substances(100,200,400 mg/L),exogenous regulating substances duration(0.5,1.0,2.0 h)in the orthogonal experiment to measure the changes of the phloem in the rooting process of cuttings soluble sugar,soluble starch,soluble protein,auxin(IAA),abscisic acid(ABA),red(GA3)enzyme element content and peroxidase(POD),polyphenol oxidase(PPO).The results showed that 27 days after cuttings,the order of soluble sugar content and peroxidase(POD)and polyphenol oxidase(PPO)activity and endogenous GA3 and IAA content from high to low degrees was IBA>NAA>6-BA,and cuttings rooting rate changes demonstrated similar laws.The change of endogenous ABA content was opposite to the other two hormones and showed inhibitory effect on the rooting of plum cuttings.
This project aims to analyze the influencing factors on Prunus cerasiferatwig cutting and rooting:types of exogenous regulation substances(IBA,NAA,6-BA),concentration of exogenous regulation substances(100,200,400 mg/L),exogenous regulating substances duration(0.5,1.0,2.0 h)in the orthogonal experiment to measure the changes of the phloem in the rooting process of cuttings soluble sugar,soluble starch,soluble protein,auxin(IAA),abscisic acid(ABA),red(GA3)enzyme element content and peroxidase(POD),polyphenol oxidase(PPO).The results showed that 27 days after cuttings,the order of soluble sugar content and peroxidase(POD)and polyphenol oxidase(PPO)activity and endogenous GA3 and IAA content from high to low degrees was IBA>NAA>6-BA,and cuttings rooting rate changes demonstrated similar laws.The change of endogenous ABA content was opposite to the other two hormones and showed inhibitory effect on the rooting of plum cuttings.
引文
[1]徐福慧.不同施肥处理对紫叶李生长生理特性的影响[D].晋中:山西农业大学,2015.
[2]王景章,丁振芬.日本落叶松、杂种落叶松嫩枝全光喷雾扦插的研究[J].东北林业大学学报,1990,18(3):9-17.
[3]朴楚炳,张有富,苗锡臣,等.促进红松插穗生根能力的研究[J].林业科技,1996,21(6):5-8.
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[17]侯凯,陈郡雯,申浩,等.川白芷内源激素的提取纯化和高效液相色谱法同步测定[J].核农学报,2013,27(5):653-657.
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[20]吴海东.六种植物的嫩枝扦插繁殖及生长调节剂对插条生理生化代谢调控的研究[D].大连:辽宁师范大学,2005.
[21]张忠微.金露梅微体快繁与扦插技术及其生根机理研究[D].保定:河北农业大学,2007.
[22]郭英超.兴安圆柏扦插生根过程中相关内源激素特性分析[J].中国农学通报,2012,28(1):44-48.
[23]郭英超.兴安圆柏嫩枝扦插过程中相关生理特性分析[J].中国园艺文摘,2012(8):7-9.
[24] Calderon Baltierra X V.Changes in peroxidase activity during root formation by Eucalyptus globules shoots raisd in vitro[J].Plant Perox Newlett,1994(4):27-29.
[25] Fitzpatrick M K,Frankel H L,Mcmaster J,et al.Integration of concurrent trauma registry and performance improvement programs[J].Journal of Trauma Nursing the Official Journal of the Society of Trauma Nurses,2000,7(4):92-97.
[26]沈永宝.秤锤树扦插繁殖技术及生根机理的研究[D].南京:南京林业大学,2009.
[2]王景章,丁振芬.日本落叶松、杂种落叶松嫩枝全光喷雾扦插的研究[J].东北林业大学学报,1990,18(3):9-17.
[3]朴楚炳,张有富,苗锡臣,等.促进红松插穗生根能力的研究[J].林业科技,1996,21(6):5-8.
[4]李庆卫.金叶锦熟黄杨、金叶侧柏扦插繁殖与离体快繁技术研究[D].北京:北京林业大学,2000.
[5] Syros T,Yupsanis T,Zafiriadis H,et al.Activity and isoforms of peroxidases,lignin and anatomy,during adventitious rooting in cuttings of Ebenus cretica L.[J].Journal of Plant Physiology,2004,161(1):69-77.
[6] Hatzilazarou S P,Syros T D,Yupsanis T A,et al.Peroxidases,lignin andanatomy during in vitro and exvitro rooting of gardenia(Gardenia jasminoides Ellis)microshoots[J].Journal of Plant Physiology,2006,163(8):827-836.
[7]曹兵.希蒙得木的扦插繁殖技术[J].南京林业大学学报(自然科学版),2003,27(4):62-66.
[8]唐前瑞.红檵木叶色变化的生理生化研究[J].林业科学,2006(2):111-115.
[9]史宝胜.紫叶李叶色生理变化及影响因素研究[D].哈尔滨:东北林业大学,2007.
[10]刘从霞.四种李属彩叶植物抗寒生理研究[D].保定:河北农业大学,2007.
[11]李单.红叶李杂交子代叶色性状及遗传多样性研究[D].保定:河北农业大学,2011.
[12]李彦慧.紫叶李叶片色素含量及叶绿素荧光动力学参数对SO2胁迫的响应[J].环境科学学报,2008,28(11):2236-2242.
[13]刘萍,王从亮,王凤格,等.鲜食糯玉米不同品种授粉后籽粒品质主要成分的变化[J].中国农业科学,2007,40(8):1817-1821.
[14]刘君,高俊凤,王燕凌.渗透胁迫下小麦幼苗根质膜ATP酶活性与膜透性的关系[J].新疆农业大学学报,2006,29(3):59-63.
[15]郝建军,黄春花,卢环,等.不同小豆品种抗旱生理指标比较的研究[J].辽宁农业科学,2012(5):21-25.
[16]刘萍,陆卫平,王凤格,等.超甜玉米品质差异及适宜采收指标的研究[J].扬州大学学报(农业与生命科学版),2007,28(1):72-76.
[17]侯凯,陈郡雯,申浩,等.川白芷内源激素的提取纯化和高效液相色谱法同步测定[J].核农学报,2013,27(5):653-657.
[18]潘健.三种柃木属植物扦插生根机理研究[D].南京:南京林业大学,2007.
[19]李焕勇.两种耐盐植物扦插繁殖技术及生根生理的研究[D].北京:林业科学研究院,2014.
[20]吴海东.六种植物的嫩枝扦插繁殖及生长调节剂对插条生理生化代谢调控的研究[D].大连:辽宁师范大学,2005.
[21]张忠微.金露梅微体快繁与扦插技术及其生根机理研究[D].保定:河北农业大学,2007.
[22]郭英超.兴安圆柏扦插生根过程中相关内源激素特性分析[J].中国农学通报,2012,28(1):44-48.
[23]郭英超.兴安圆柏嫩枝扦插过程中相关生理特性分析[J].中国园艺文摘,2012(8):7-9.
[24] Calderon Baltierra X V.Changes in peroxidase activity during root formation by Eucalyptus globules shoots raisd in vitro[J].Plant Perox Newlett,1994(4):27-29.
[25] Fitzpatrick M K,Frankel H L,Mcmaster J,et al.Integration of concurrent trauma registry and performance improvement programs[J].Journal of Trauma Nursing the Official Journal of the Society of Trauma Nurses,2000,7(4):92-97.
[26]沈永宝.秤锤树扦插繁殖技术及生根机理的研究[D].南京:南京林业大学,2009.