光照对金叶风箱果叶色恢复及光系统Ⅱ的影响
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摘要
以3年生金叶风箱果(Physocarpus opulifolius‘Lutein’)为试材,先遮荫使其叶色变为绿色,然后再进行全光照处理,旨在探明光照对金叶风箱果叶色恢复及光系统Ⅱ的影响。结果表明:光照处理后,金叶风箱果叶片迅速恢复为黄色。叶绿素a、叶绿素b以及叶绿素总量均在第2天出现最大值,分别比处理前增加了27.79%、34.75%、28.97%,差异极显著(P<0.01);净光合速率(Pn)和气孔导度(gs)整体均呈现先上升后下降的趋势;随处理时间的延长,初始荧光(Fo)的值表现为先降低后升高,最大荧光(Fm)的值整体表现为下降的趋势,PSⅡ最大光化学效率(Fv/Fm)的值先升高后降低,在处理第1天时略有升高,比处理前增加了1.41%,无显著差异,后逐渐下降;见光后,与能量分配比率有关的快速叶绿素荧光参数φPo、Ψo、φEo的值均出现减小的趋势,而φDo的值则逐渐增大;光照处理后,单位反应中心吸收的能量(APS/RC)增大,单位反应中心用于热耗散的能量(DIo/RC)升高,表明单位PSⅡ反应中心所吸收的光能分配到电子传递的能量减少,而是更多以热耗散的形式散发出来。综上可知,充足的光照有利于金叶风箱果叶片呈色,但其光系统Ⅱ在强光照射下短期会受到一定损伤。
In order to study the effect of light on leaf color restoration and photosystemsⅡ,the three-year-old Physocarpus opulifolius‘Lutein'was used as the material in the experiment,and the leaves of Physocarpus opulifolius‘Lutein'were first shasded till turning green,and then exposed to full light.The results indicated that:the leaf color of this plant rapidly restored to yellow after the light treatment.The content of chlorophyll a,chlorophyll b and chlorophyll were all the largest at 2 nd day,which significantly increased by 27.79%,34.75%,and 28.97% compared with the pretreatment.The photosynthetic rate(Pn)and stomatal conductance(gs)both showed an increase first before the decrease.The initial fluorescence intensity(Fo)showed a decrease first before the increase.The maximal fluorescence intensity(Fm)decreased on the whole and the maximal phatochemical efficiency(Fv/Fm)was increased first and then decreased,which was slightly higher in the treatment of 1 st day with an increase of 1.41%compare to the pretreatment,and then gradually decreased with the treatment time prolonged.After being exposed to light,the value of chlorophyll fluorescence parametersφPo,ΨoandφEoall decreased,while the value ofφDoincreased.After the light treatment,the absorption flux per reaction center(ABS/RC)increased and the dissipated energy flux per reaction center(DIo/RC)increased significantly,which indicated the light energy of absorption flux per PSⅡreaction center was less distributed to the electron for transferring energy,but more transformed in the form of heat dissipation.It is considered that full light is beneficial to leaves coloration,but its photosystem Ⅱ can be damaged under strong light within a short period of time.
In order to study the effect of light on leaf color restoration and photosystemsⅡ,the three-year-old Physocarpus opulifolius‘Lutein'was used as the material in the experiment,and the leaves of Physocarpus opulifolius‘Lutein'were first shasded till turning green,and then exposed to full light.The results indicated that:the leaf color of this plant rapidly restored to yellow after the light treatment.The content of chlorophyll a,chlorophyll b and chlorophyll were all the largest at 2 nd day,which significantly increased by 27.79%,34.75%,and 28.97% compared with the pretreatment.The photosynthetic rate(Pn)and stomatal conductance(gs)both showed an increase first before the decrease.The initial fluorescence intensity(Fo)showed a decrease first before the increase.The maximal fluorescence intensity(Fm)decreased on the whole and the maximal phatochemical efficiency(Fv/Fm)was increased first and then decreased,which was slightly higher in the treatment of 1 st day with an increase of 1.41%compare to the pretreatment,and then gradually decreased with the treatment time prolonged.After being exposed to light,the value of chlorophyll fluorescence parametersφPo,ΨoandφEoall decreased,while the value ofφDoincreased.After the light treatment,the absorption flux per reaction center(ABS/RC)increased and the dissipated energy flux per reaction center(DIo/RC)increased significantly,which indicated the light energy of absorption flux per PSⅡreaction center was less distributed to the electron for transferring energy,but more transformed in the form of heat dissipation.It is considered that full light is beneficial to leaves coloration,but its photosystem Ⅱ can be damaged under strong light within a short period of time.
引文
[1]王振兴,于云飞,陈丽,等.彩叶植物叶片色素组成、结构以及光合特性的研究进展[J].植物生理学报,2016,52(1):1-7.
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[4]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.
[5]宋婷,张谧,高吉喜,等.快速叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J].生物学杂志,2011,28(6):81-86.
[6]贾浩,郝建博,曹洪波,等.遮荫对‘保佳红’桃树叶片快速叶绿素荧光诱导动力学曲线的影响[J].西北植物学报,2015,35(9):1861-1867.
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[12]杨露,李芸,梁文华,等.光照对金叶风箱果叶色恢复及光系统Ⅱ的影响[J].河北农业大学学报,2017,40(4):72-77+83.
[13]张智顺,张庆费,夏擂,等.遮阴对几种绿化植物光合特性和生长的影响[J].东北林业大学学报,2010,38(3):47-49+72.
[14]孙小玲,许岳飞,马鲁沂,等.植株叶片的光合色素构成对遮阴的响应[J].植物生态学报,2010,34(8):989-999.
[15]黄俊玲,王妍.干热河谷乡土树种叶绿素荧光特征对水分胁迫的响应[J].西北植物学报,2015,35(12):2505-2512.
[16]张诚君,王磊,段书延,等.‘巨峰’葡萄盛花后弱光胁迫对叶片光合生理及光合酶基因表达的影响[J].园艺学报,2017,44(8):1450-1462.
[17]李国栋,田曼青,沈仁芳.拟南芥独脚金内酷突变体叶绿素荧光特性分析[J].浙江农林大学学报,2017,34(1):36-41.
[18]黄秋娴,赵顺,刘春梅,等.遮荫处理对铁尾矿基质臭柏实生苗快速叶绿素荧光特性的影响[J].林业科学,2015,51(6):17-26.
[19]汤正辉,沈植国,罗颖,等.遮荫对风丹白光合特性的影响[J].西北林学院学报,2017,32(5):46-51.
[20]Dem ming A B,Adams W W,Baker D H,etal.U-sing chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation[J].Physiologic Plant,1996,98:253-264.
[21]李泽,谭晓风,卢馄,等.干旱胁迫对两种油桐幼苗生长、气体交换及叶绿素荧光参数的影响[J].生态学报,2017,37(5):1515-1524.
[22]胡文海,张斯斯,肖宜安,等.两种杜鹃花属植物对长期遮阴后全光照环境的生理响应及其光保护机制[J].植物生态学报,2015,39(11):1093-1100.
[2]王艺,韦小丽.不同光照对植物生长、生理生化和形态结构影响的研究进展关[J].山地农业生物学报,2010,29(4):353-359.
[3]Kim S J,Yu D J,Kim T C,et al.Growth and photosynthetic characteristics of blueberry(Vaccinium corymbosumcv.Bluecrop)under various shade levels[J].Scientia Horticulturae,2011,129(3):486-492.
[4]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.
[5]宋婷,张谧,高吉喜,等.快速叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J].生物学杂志,2011,28(6):81-86.
[6]贾浩,郝建博,曹洪波,等.遮荫对‘保佳红’桃树叶片快速叶绿素荧光诱导动力学曲线的影响[J].西北植物学报,2015,35(9):1861-1867.
[7]张天麟.园林树木1000种[M].北京学术出版社,1990.
[8]杜丽雁,刘春静,李永儒,等.金叶风箱果嫩枝扦插试验[J].辽宁林业科技,2006(2):26-27.
[9]王占龙.金叶风箱果组培技术研究[J].防护林科技,2013(8):26-27+37.
[10]刘晓东,翟晓宇,施冰.金叶风箱果和金叶风箱果的抗寒性[J].东北林业大学学报,2011,39(4):18-20.
[11]杨露,于晓跃,刘煜光,等.遮荫对2种彩叶风箱果叶色及光合特性的影响[J].河北农业大学学报,2016,39(5):75-81.
[12]杨露,李芸,梁文华,等.光照对金叶风箱果叶色恢复及光系统Ⅱ的影响[J].河北农业大学学报,2017,40(4):72-77+83.
[13]张智顺,张庆费,夏擂,等.遮阴对几种绿化植物光合特性和生长的影响[J].东北林业大学学报,2010,38(3):47-49+72.
[14]孙小玲,许岳飞,马鲁沂,等.植株叶片的光合色素构成对遮阴的响应[J].植物生态学报,2010,34(8):989-999.
[15]黄俊玲,王妍.干热河谷乡土树种叶绿素荧光特征对水分胁迫的响应[J].西北植物学报,2015,35(12):2505-2512.
[16]张诚君,王磊,段书延,等.‘巨峰’葡萄盛花后弱光胁迫对叶片光合生理及光合酶基因表达的影响[J].园艺学报,2017,44(8):1450-1462.
[17]李国栋,田曼青,沈仁芳.拟南芥独脚金内酷突变体叶绿素荧光特性分析[J].浙江农林大学学报,2017,34(1):36-41.
[18]黄秋娴,赵顺,刘春梅,等.遮荫处理对铁尾矿基质臭柏实生苗快速叶绿素荧光特性的影响[J].林业科学,2015,51(6):17-26.
[19]汤正辉,沈植国,罗颖,等.遮荫对风丹白光合特性的影响[J].西北林学院学报,2017,32(5):46-51.
[20]Dem ming A B,Adams W W,Baker D H,etal.U-sing chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation[J].Physiologic Plant,1996,98:253-264.
[21]李泽,谭晓风,卢馄,等.干旱胁迫对两种油桐幼苗生长、气体交换及叶绿素荧光参数的影响[J].生态学报,2017,37(5):1515-1524.
[22]胡文海,张斯斯,肖宜安,等.两种杜鹃花属植物对长期遮阴后全光照环境的生理响应及其光保护机制[J].植物生态学报,2015,39(11):1093-1100.