基于SRY基因鉴定新疆野兔性别
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摘要
为得到形态上性别鉴定特征十分缺乏的新疆野兔样本的性别数据,本研究采用双重PCR法同时扩增SRY基因和APP基因,通过分析PCR产物,对采自新疆9个地区共121例未知性别的野兔样本进行性别鉴定。在验证PCR结果的可靠性后,鉴定出雌性样本69例,雄性样本52例,性别比例经统计学分析后接近1∶1,符合实际。这为后续研究新疆野兔的物种分类、群体遗传学和分子进化等方面提供了基础数据,也在兔类资源的管理保护和开发利用方面有着一定的实际意义。
Xinjiang hares lack features of external morphology that might enable gender determination. To identify the gender of Xinjiang hares,we applied the duplex PCR method to amplify the SRY and APP genes simultaneously. After verifying the correction and reliability of PCR results by gender-known samples,a total of 121 gender-unknown hare samples from 9 regions of Xinjiang were tested for gender determination. Sixty-nine females and 52 males were identified,and the sex ratio approached 1∶ 1 in statistical analysis. These results provide a foundation for future studies on species classification,population genetics,and molecular evolution of Xinjiang hares. We hope this will enhance management and protection of hare resources.
Xinjiang hares lack features of external morphology that might enable gender determination. To identify the gender of Xinjiang hares,we applied the duplex PCR method to amplify the SRY and APP genes simultaneously. After verifying the correction and reliability of PCR results by gender-known samples,a total of 121 gender-unknown hare samples from 9 regions of Xinjiang were tested for gender determination. Sixty-nine females and 52 males were identified,and the sex ratio approached 1∶ 1 in statistical analysis. These results provide a foundation for future studies on species classification,population genetics,and molecular evolution of Xinjiang hares. We hope this will enhance management and protection of hare resources.
引文
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[11]阿尔孜古力·沙塔尔,迪力夏提·阿不力孜,孜比尔妮莎·吾不力,等.扩增SRY基因鉴定塔里木兔性别的研究[J].新疆农业科学,2010,47(5):992-995.
[12]Bryja J,KoneˇcnA.Fast sex identification in wild mammals using PCR amplification of the SRY gene[J].Folia Zoologica,2003,52(3):269-274.
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[14]赵建军.兔早期胚胎PCR性别鉴定体系的建立[D].兰州:甘肃农业大学,2006.
[15]黄银花,胡晓湘,徐慰倬,等.影响多重PCR扩增效果的因素[J].遗传,2003,25(1):65-68.
[16]Rowan W,Keith L B.Reproductive potential and sex ratios of snowshoe hares in northern Alberta[J].Canadian Journal of Zoology,1956,34(4):273-281.
[17]Hewson R.Behaviour,population changes and dispersal of mountain hares(Lepus timidus)in Scotland[J].Journal of Zoology,1990,220(2):287-309.
[18]卢欣,郭东龙.野兔种群生态学研究概况[J].山西大学学报(自然科学版),1992,15(2):206-213.
[2]阿布里米提.国家级重点保护的新疆野生动物[J].干旱区研究,1990,7(2):24.
[3]Sinclair A H,Berta P,Palmer M S,et al.A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif[J].Nature,1990,346(6281):240-244.
[4]Koopman P,Gubbay J,Vivian N,et al.Male development of chromosomally female mice transgenic for Sry[J].Nature,1991,351(6322):117-121.
[5]张秀慧,毕为民.染色体检测技术的发展及其在遗传病诊断中的应用[J].医学研究杂志,2011,40(6):4-8;3.
[6]郭海燕,张昊,李拥军,等.性别控制技术及其在家畜生产中的应用[J].安徽农业科学,2016,44(25):116-118.
[7]孟醒,张正茂,赵强.家畜早期胚胎性别鉴定技术的研究进展[J].青海畜牧兽医杂志,2015,45(5):49-53.
[8]张秀华,吴登俊.性别决定机理与性别鉴定的研究进展[J].中国畜牧兽医,2004,31(7):23-26.
[9]Mengoni C,Mucci N,Randi E.Sex identification in four leporid species(Lepus corsicanus,Lepus europaeus,Lepus timidus and Lepus capensis mediterraneus)[J].Conservation Genetics Resources,2015,7(3):619-621.
[10]孙志明,徐琪,徐禄,等.基于CHD基因和EE0.6基因遗传多态鉴定白枕鹤、丹顶鹤性别研究报告[J].野生动物学报,2018,39(1):151-153.
[11]阿尔孜古力·沙塔尔,迪力夏提·阿不力孜,孜比尔妮莎·吾不力,等.扩增SRY基因鉴定塔里木兔性别的研究[J].新疆农业科学,2010,47(5):992-995.
[12]Bryja J,KoneˇcnA.Fast sex identification in wild mammals using PCR amplification of the SRY gene[J].Folia Zoologica,2003,52(3):269-274.
[13]Nalivaeva N N,Turner A J.The amyloid precursor protein:a biochemical enigma in brain development,function and disease[J].FEBS Letters,2013,587(13):2046-2054.
[14]赵建军.兔早期胚胎PCR性别鉴定体系的建立[D].兰州:甘肃农业大学,2006.
[15]黄银花,胡晓湘,徐慰倬,等.影响多重PCR扩增效果的因素[J].遗传,2003,25(1):65-68.
[16]Rowan W,Keith L B.Reproductive potential and sex ratios of snowshoe hares in northern Alberta[J].Canadian Journal of Zoology,1956,34(4):273-281.
[17]Hewson R.Behaviour,population changes and dispersal of mountain hares(Lepus timidus)in Scotland[J].Journal of Zoology,1990,220(2):287-309.
[18]卢欣,郭东龙.野兔种群生态学研究概况[J].山西大学学报(自然科学版),1992,15(2):206-213.