溃结安康汤对溃疡性结肠炎模型大鼠MPO水平及ITF mRNA表达的影响
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摘要
目的:观察溃结安康汤对大鼠溃疡性结肠炎(UC)的治疗作用,并探讨其可能机制。方法:48只清洁级SD大鼠随机分为4组:正常组、模型组、溃结安康汤组、柳氮磺吡啶(SASP)组。造模并治疗21d后,进行疾病活动指数(DAI)评分,观察结肠HE染色病理涂片,髓过氧化物酶(MPO)水平,以及肠三叶因子(ITF)mRNA表达。结果:与模型组相比,溃结安康汤组DAI评分显著降低(P<0.01),结肠HE染色病理示炎症细胞明显减少,MPO水平显著降低(P<0.01),ITF mRNA表达显著升高(P<0.01)。结论:溃结安康汤可以显著改善UC大鼠症状、病理形态,通过降低MPO水平,上调ITF mRNA表达,从而达到抑制炎症的效果。
Objective: To observe the therapeutic effect of Kuijie Ankang Decoction on ulcerative colitis(UC) in rats and explore its possible mechanism. Methods: Forty-eight clean SD rats were randomly divided into four groups: normal group, model group, Kuijie Ankang Decoction group and SASP group. After 21 days of treatment, disease activity index(DAI) was scored to observe the expression of intestinal trefoil factor(ITF) and myeloperoxidase(MPO) in HE stained pathological smears of colon.Results: Compared with the model group, the DAI score of Kuijie Ankang Decoction group was lower(P<0.01), the inflammatory cells and MPO levels were significantly reduced(P<0.01), and the expression of ITF mRNA was significantly increased(P<0.01).Conclusion: Kuijie Ankang Decoction can significantly improve the symptoms and pathological morphology of UC rats. It can inhibit inflammation by lowering MPO level and increasing ITF gene expression.
Objective: To observe the therapeutic effect of Kuijie Ankang Decoction on ulcerative colitis(UC) in rats and explore its possible mechanism. Methods: Forty-eight clean SD rats were randomly divided into four groups: normal group, model group, Kuijie Ankang Decoction group and SASP group. After 21 days of treatment, disease activity index(DAI) was scored to observe the expression of intestinal trefoil factor(ITF) and myeloperoxidase(MPO) in HE stained pathological smears of colon.Results: Compared with the model group, the DAI score of Kuijie Ankang Decoction group was lower(P<0.01), the inflammatory cells and MPO levels were significantly reduced(P<0.01), and the expression of ITF mRNA was significantly increased(P<0.01).Conclusion: Kuijie Ankang Decoction can significantly improve the symptoms and pathological morphology of UC rats. It can inhibit inflammation by lowering MPO level and increasing ITF gene expression.
引文
[1]杨佳,范恒.复方苦参汤对溃疡性结肠炎大鼠炎性反应和氧化应激的影响.中华中医药杂志,2017,32(8):3457-3461
[2]中国中西医结合学会消化系统疾病专业委员会.溃疡性结肠炎中西医结合诊疗指南(草案).中国中西医结合消化杂志,2011,19(1):61-65
[3]柳越冬,陶弘武,田振国.慢性非特异性溃疡性结肠炎中西医结合治疗进展.中华中医药学刊,2009,27(5):1002-1004
[4]Wang Y H,Ge B,Yang X L,et al.Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with TNBS-induced recurrent ulcerative colitis.International Immunopharmacology,2011,11(10):1620-1627
[5]Li W G,Zhang X Y,Wu Y J,et al.Anti-inflammatory effect and mechanism of proanthocyanidins from grape seeds.Acta Pharmacologica Sinica,2001,22(12):1117-1120
[6]王坤,宣秀敏,王莲,等.溃疡性结肠炎大鼠外周血CD4+CD25+Treg的变化及相关性研究.安徽医科大学学报,2012,47(3):354-356
[7]吴崑,严红丽,穆敬平.MMP-2、MMP-9与溃疡性结肠炎患者肠黏膜通透性的相关性研究.临床与病理杂志,2016,36(4):384-388
[8]Sanchez-Munoz F,Dominguez-Lopez A,Yamamoto-Furusho J K.Role of cytokines in inflammatory bowel disease.World J Gastroenterol,2008,14(27):4280-4288
[9]陈美娅,张靖,任建林.三叶因子3研究进展.世界华人消化杂志,2008,16(20):2267-2273
[10]LiWang,JianLi,QingLi,et al.Morphological changes of cell proliferation and apoptosis in rat jejunal mucosa at different ages.World J Gastroenterol,2003,9(9):2060-2064
[2]中国中西医结合学会消化系统疾病专业委员会.溃疡性结肠炎中西医结合诊疗指南(草案).中国中西医结合消化杂志,2011,19(1):61-65
[3]柳越冬,陶弘武,田振国.慢性非特异性溃疡性结肠炎中西医结合治疗进展.中华中医药学刊,2009,27(5):1002-1004
[4]Wang Y H,Ge B,Yang X L,et al.Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with TNBS-induced recurrent ulcerative colitis.International Immunopharmacology,2011,11(10):1620-1627
[5]Li W G,Zhang X Y,Wu Y J,et al.Anti-inflammatory effect and mechanism of proanthocyanidins from grape seeds.Acta Pharmacologica Sinica,2001,22(12):1117-1120
[6]王坤,宣秀敏,王莲,等.溃疡性结肠炎大鼠外周血CD4+CD25+Treg的变化及相关性研究.安徽医科大学学报,2012,47(3):354-356
[7]吴崑,严红丽,穆敬平.MMP-2、MMP-9与溃疡性结肠炎患者肠黏膜通透性的相关性研究.临床与病理杂志,2016,36(4):384-388
[8]Sanchez-Munoz F,Dominguez-Lopez A,Yamamoto-Furusho J K.Role of cytokines in inflammatory bowel disease.World J Gastroenterol,2008,14(27):4280-4288
[9]陈美娅,张靖,任建林.三叶因子3研究进展.世界华人消化杂志,2008,16(20):2267-2273
[10]LiWang,JianLi,QingLi,et al.Morphological changes of cell proliferation and apoptosis in rat jejunal mucosa at different ages.World J Gastroenterol,2003,9(9):2060-2064