摘要
自2011年下半年以来全国多个省份使用Bartha-K61疫苗(gE/gI双缺失)免疫的猪场陆续出现了母猪繁殖障碍、仔猪神经症状及死亡、伪狂犬gE抗体阴性猪群突然转阳的问题。同时有的猪场内出现犬、猫、鼠等死亡的现象,经检测都是由变异伪狂犬病病毒(vPRV)引起。为了解山东省猪伪狂犬病病毒(PRV)近期流行特征,本试验对2017年上半年来自山东省不同地区的12份伪狂犬疑似病料进行了PRV的分离鉴定,并对分离株的gE基因进行了序列测定及遗传变异分析。结果表明,共分离到9株PRV,其核苷酸和氨基酸序列的同源性分别为97.5%~99.4%和97.2%~99.1%,与参考毒株其核苷酸和氨基酸序列的同源性分别为97.5%~99.8%和96.8%~99.2%,并且9株分离株与国内报道的PRV变异株的核苷酸和氨基酸序列的同源性均高于欧美毒株。gE基因的遗传进化树分析表明,本试验中9株分离株相互之间遗传距离较近,与国内PRV变异株处在同一分支,而与欧美毒株遗传距离较远。
In latter 2011,pig farms in many provinces of China vaccinated with the Bartha-K61vaccine(gE/gI double deletion)showed reproductive disorders of sows,neurologic symptoms and death of piglets,and the sudden transformation of gE antibody from negative to positive.Meanwhile,there were the death of dogs,cats,mice and other in some pig farms,which were caused by the variant pseudorabies virus(vPRV)through detection.To understand the recent epidemic characteristics of pseudorabies virus(PRV)in Shandong province,our research conducted isolation,identification and phylogenetic analysis of gE gene of pseudorabies virus on 12 samples collected from parts of Shandong province in the first half of 2017.The results showed that 9strains of PRV were isolated and they shared nucleotide and amino acid homologies of 97.5%-99.4% and 97.2%-99.1%.While nucleotide and amino acid homologies were 97.5%-99.8% and 96.8%-99.2% when compared with the reference strains,and the 9isolates shared higher nucleotide and amino acid identities with Chinese variant strains than Euramerican strains.Phylogenetic analysis of gE gene showed that the 9isolates shared close genetic distance with vPRV and were clustered into the same branch,while were distant from Euramerican strains.
引文
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