低温环境对民猪免疫应答的影响
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摘要
为研究低温环境条件下免疫应答对民猪激素水平的影响,选择健康的仔猪(民猪及大白猪),达到21日龄时,接种猪瘟兔化弱毒疫苗,达到48日龄时,检测的猪瘟抗体,根据结果选择体重、抗体水平接近的仔猪20头,大白猪仔猪20头,随机分组,分别为低温环境两组(1组、2组)及适温环境两组(3组、4组),其中1组为10头民猪,2组为10头大白猪,3组为10头民猪,4组为10头大白猪。试验期14 d,以猪瘟疫苗接种作为免疫应答手段,用ELISA方法对血清中方法检测干扰素-α(IFN-α)、白细胞介素6(IL-6)、猪瘟抗体(CSF)含量进行测定。结果表明:2组的IFN-α含量在14 d时、IL-6含量在7 d和14 d时、CSF在14 d时显著低于其他3组(P<0.05)。说明低温环境对民猪免疫应答的影响程度低于大白猪,民猪的抗冷应激能力强于大白猪。
In order to study the effect of immune response on the level of Min pig hormone in low temperature environment,clinical healthy Min piglets and Large White piglets were selected,were vaccinated with the swine plague vaccine at 21 days of age,blood samples were collected for detection of swine plague antibody levels at 48 days of age,20 Min piglets and 20 White piglets with similar antibody levels and similar body weight were used and were divided into four groups randomly:low and normal group,normal temperature group in the two groups,the first group of 10 Min pigs were raised at low temperature,the second group of 10 White pigs were feeding at low temperature,the third group of 10 Min pigs were raising in normal temperature,the fourth group of 10 white pigs were feeding in normal temperature.To declare ELISA result,Interferon-α(IFN-α),Interleukin-6(IL-6)and Swine plague antibody(CSF)were determined.The results showed that 14 days of immunization,IFN-αconcentrations of 2 group reduced than the other three groups respectively(P<0.05).IL-6 concentration significantly reduced in 7 days and 14 days immunization,CSF concentrations of 2 group was significantly reduced than the other three groups(P<0.05).The results showed that the immune response in low temperature environment had less influence on min pigs than the large white pigs,and the min pigs were more resistant to cold stress than the large white pigs.
In order to study the effect of immune response on the level of Min pig hormone in low temperature environment,clinical healthy Min piglets and Large White piglets were selected,were vaccinated with the swine plague vaccine at 21 days of age,blood samples were collected for detection of swine plague antibody levels at 48 days of age,20 Min piglets and 20 White piglets with similar antibody levels and similar body weight were used and were divided into four groups randomly:low and normal group,normal temperature group in the two groups,the first group of 10 Min pigs were raised at low temperature,the second group of 10 White pigs were feeding at low temperature,the third group of 10 Min pigs were raising in normal temperature,the fourth group of 10 white pigs were feeding in normal temperature.To declare ELISA result,Interferon-α(IFN-α),Interleukin-6(IL-6)and Swine plague antibody(CSF)were determined.The results showed that 14 days of immunization,IFN-αconcentrations of 2 group reduced than the other three groups respectively(P<0.05).IL-6 concentration significantly reduced in 7 days and 14 days immunization,CSF concentrations of 2 group was significantly reduced than the other three groups(P<0.05).The results showed that the immune response in low temperature environment had less influence on min pigs than the large white pigs,and the min pigs were more resistant to cold stress than the large white pigs.
引文
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[3] Mariusz C,Magdalena C,Ksiazek A,et al.Delayed effects of cold stress on immune response in laboratory mice[J].Proceeding of the Royal Society B:Biological Sciences,2002,269(1499):1493-1497.
[4] 李士泽,袁学军,杨玉英.冷暴露对健康雏鸡某些血液生化指标及胃肠道黏充血的影响[J].中国应用生理学杂志,2002,18(2):148-158.
[5] 吕彩霞,王永杰,李瑞刚.冷应激反应的神经机制及对免疫系统的影响[J].兽医导刊,2008(3):27.
[6] Klasing K C,Barnes D M. Decreased aminoacid requirements of growing chicks due to immunologic stress [J].The Journal of Nutrition,1988,118(9):1158-1164.
[7] Johnson R W.Inhibition of growth by pro-inflammatory cytokines:An integrated view[J].Journal of Animal Science,1997,75(5):1244-1255.
[8] 贾震虎.免疫应激鸡免疫器官病理组织学研究[J].畜禽业,2005(l):50-61.
[9] Meager A.Biologicai assays for interferons[J].Journal of Immunological Methods,2002,261(1/2):21-36.
[10] Johnson H M,Bazer F W,Szente B E,et al.How interferons fight disease[J].Scientific American,1994,270(5):68-75.
[11] 谢庆阁,翟中和.畜禽重大疫病免疫防制研究进展[M].北京:中国农业科技出版社,1996:64-92.
[12] Taga T,Kishimoto T.Gp130 and the interleukin-6 family of cytokines[J].Annual Review of Immunology,2003,15(21):797-819.
[13] Dienz O,Rincon M.The effect of IL-6 on CD4 T cell responses[J].Clinical Immunology,2009,130(1):27-33.
[14] 陈萍,杨焕民.急性冷暴露对仔猪血浆中IL-2、IL-6、ACTH和皮质醇水平的影响[J].应用与环境生物学报,2009.15(1):91-94.
[15] 彭福刚,孙金艳,李忠秋.低温环境下免疫应答对民猪血清激素水平的影响[J].黑龙江畜牧兽医,2018(10):50-52.