锌离子螯合修饰河蚬抗氧化肽的工艺
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摘要
确定锌离子螯合修饰河蚬抗氧化肽的最佳工艺条件。以螯合率为响应值,通过单因素试验和响应曲面分析方法优化河蚬抗氧化肽螯合锌的工艺参数,确定最优螯合工艺条件:pH 5.07,抗氧化肽与锌离子质量比2.18︰1,螯合时间46.50 min,螯合温度52.65℃。在最优工艺条件下,河蚬抗氧化肽螯合锌的螯合率为81.78%。
The optimal chelation conditions of antioxidant peptides from Corbicula fluminea chelated with zinc ions were determined. Taking the chelation rate as the response value, the best chelation technology of Corbicula fluminea antioxidant peptides was optimized by single factor experiment and response surface methodology. The optimal chelation technological parameters were as follows: pH 5.07, Corbicula fluminea antioxidant peptides/zinc ions ratio 2.18︰1, reaction time 46.50 min, and reaction temperature 52.65 ℃. Under the optimal process conditions, the chelation rate was 81.78%.
The optimal chelation conditions of antioxidant peptides from Corbicula fluminea chelated with zinc ions were determined. Taking the chelation rate as the response value, the best chelation technology of Corbicula fluminea antioxidant peptides was optimized by single factor experiment and response surface methodology. The optimal chelation technological parameters were as follows: pH 5.07, Corbicula fluminea antioxidant peptides/zinc ions ratio 2.18︰1, reaction time 46.50 min, and reaction temperature 52.65 ℃. Under the optimal process conditions, the chelation rate was 81.78%.
引文
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[3]王子怀,胡晓,李来好,等.肽-金属离子螯合物的研究进展[J].食品工业科技,2014,35(8):359-363.
[4]刘温,楼乔明,杨文鸽.多肽金属元素螯合物研究进展[J].食品与发酵工业,2014,40(4):142-146.
[5]尤娟.鲢鱼鱼肉蛋白抗氧化肽的制备及其糖基化产物功能特性的研究[D].北京:中国农业大学,2014.
[6]DENG S G,HUO J C,XIE C.Preparation by enzymolysis and bioactivity of iron complex of fish protein hydrolysate(FeFPH)from low value fish[J].Chinese Journal of Oceanology and Limnology,2008,26(3):300-306.
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[9]李杨,齐宝坤,隋晓楠,等.超声辅助酶解制备红豆多肽及其抗氧化性的研究[J].食品工业科技,2016,1(37):232-237.
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