蛋氨酸缺乏对雏鸡盲肠扁桃体T淋巴细胞亚群和细胞因子的影响
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摘要
为了研究蛋氨酸缺乏对雏鸡盲肠扁桃体T淋巴细胞亚群和细胞因子的影响,试验选取100只1日龄健康Cobb(科宝)雏鸡分为对照组和蛋氨酸缺乏组,分别采食对照日粮和蛋氨酸缺乏日粮,试验期为42 d,分别于14、28和42日龄对雏鸡进行剖检,取盲肠扁桃体样品进行酶联免疫吸附试验(ELISA)和实时荧光定量PCR(qRT-PCR)检测T淋巴细胞亚群和细胞因子。结果表明:与对照组相比,蛋氨酸缺乏组中盲肠扁桃体CD3+、CD3++CD4+、CD3++CD8+以及CD4+/CD8+比例均显著或极显著降低(P<0.05或P<0.01)。细胞因子白介素2(IL-2)、IL-6、IL-10、IL-17、IFN-γ、TNF-α(LITAF)的含量及mRNA表达水平也呈现不同程度的下降(P<0.05或P<0.01)。说明蛋氨酸缺乏会导致盲肠扁桃体T淋巴细胞亚群和细胞因子下降,进而影响雏鸡的特异性免疫功能。
To study the effects of methionione deficiency on T lymphocyte population and cytokines on cecal tonsil in broilers, 100 one-day-age healthy Cobb broilers were selected and divided into two groups and fed with a control diet and Met-deficient diet for 42 days. During the period of experiment, the T lymphocyte population and cytokines of cecal tonsil were detected by enzyme-linked immunosorbent assays(ELISA) and quantitative real-time PCR(qRT-PCR) at 14,28 and 42 days of age. The result showed that the population of CD3+, CD3++CD4+, CD3++CD8+and the rate of CD4+/CD8+were decreased or significantly decreased(P<0.05 or P<0.01) in Met deficiency group when compared with those of the control group. The IL-2, IL-6, IL-10, IL-17, IFN-γ, TNF-α(LITAF) mRNA expression level were also reduced or significantly reduced in Met deficiency group(P<0.05 or P<0.01).It′s indicated that Methionine deficiency might cause the decrease on T lymphocyte subgroup and cytokines on caecal tonsil, and then thespecificimmune function of broilers was affected.
To study the effects of methionione deficiency on T lymphocyte population and cytokines on cecal tonsil in broilers, 100 one-day-age healthy Cobb broilers were selected and divided into two groups and fed with a control diet and Met-deficient diet for 42 days. During the period of experiment, the T lymphocyte population and cytokines of cecal tonsil were detected by enzyme-linked immunosorbent assays(ELISA) and quantitative real-time PCR(qRT-PCR) at 14,28 and 42 days of age. The result showed that the population of CD3+, CD3++CD4+, CD3++CD8+and the rate of CD4+/CD8+were decreased or significantly decreased(P<0.05 or P<0.01) in Met deficiency group when compared with those of the control group. The IL-2, IL-6, IL-10, IL-17, IFN-γ, TNF-α(LITAF) mRNA expression level were also reduced or significantly reduced in Met deficiency group(P<0.05 or P<0.01).It′s indicated that Methionine deficiency might cause the decrease on T lymphocyte subgroup and cytokines on caecal tonsil, and then thespecificimmune function of broilers was affected.
引文
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[2] WATERLAND R A. Assessing the effects of high methionine intake on DNA methylation[J]. The Journal of Nutrition, 2006, 136(6):1706S-1710S.
[3]蒋蓓蕾,吴邦元,杜光波.蛋氨酸缺乏对肉鸡肝脏和肾脏功能的影响[J].中国家禽,2018,40(3):59-62.
[4]吴邦元.蛋氨酸缺乏对雏鸡免疫器官及免疫功能影响的研究[D].雅安:四川农业大学,2011.
[5]杨丽妙.淋巴细胞浸润在输卵管妊娠发生中的可能作用[D].石家庄:河北医科大学,2015.
[6] HONG Y C, PAIK S R, LEE H J, et al. Magnesium inhibits nickel-induced genotoxicity and formation of reactive oxygen[J]. Environmental Health Perspectives, 1997,105(7):744-748.
[7] SHINI S, KAISER P. Effects of stress, mimicked by administration of corticosterone in drinking water, on the expression of chicken cytokine and chemokine genes in lymphocytes[J]. Stress:The International Journal on the Biology of Stress, 2009, 12(5):388-399.
[8] IQBAL M, PHILBIN V J, SMITH A L. Expression patterns of chicken Toll-like receptor mRNA in tissues, immune cell subsets and cell lines[J]. Veterinary Immunology and Immunopathology, 2005, 104(1-2):117-127.
[9]金伯泉.医学免疫学[M]. 6版.北京:人民卫生出版社,2013:106-108.
[10]程虹,乔文斌,黄艳春.检测外周血T淋巴细胞亚群、Th17和Treg细胞在初诊多发性骨髓瘤患者中的临床意义研究[J].国际检验医学杂志,2017,38(24):3401-3403.
[11] RAJA J, DENTON C P. Cytokines in the immunopathology of systemic sclerosis[J]. Seminars in Immunopathology,2015, 37(5):543-557.
[12] OH B R. Label-free biosensors for cyokine detection[D].Michigan:University of Michigan, 2016.
[13]周骏,王婷,姚瑞芹,等. IL-2抑制小鼠CD4+CD62L+T细胞分化为Th17细胞[J].基础医学与临床,2016,36(1):19-23.
[14]陈思铭,成浩,张芸,等. IL-17、IL-6对DSS诱导的小鼠慢性结肠炎结肠癌模型的调控作用的初步研究[J].南开大学学报(自然科学版),2015(1):7-13.