纳米ZnO对中华圆田螺的氧化应激效应
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摘要
以淡水螺为受试生物,研究了中华圆田螺(Cipangopaludina Cahayensis)肝脏中自由基强度、抗氧化酶活性和丙二醛(MDA)含量纳米ZnO暴露21 d时的变化情况.结果表明,纳米ZnO可显著诱导中华圆田螺产生羟基(·OH)自由基;超氧化物歧化酶(SOD)活性和MDA含量的变化趋势相同,0.1—1 mg·L~(-1)暴露下活性随浓度增加逐渐增加,2 mg·L~(-1)时有所回落;过氧化氢酶(CAT)活性呈诱导状态,随浓度增加活性缓慢增大;谷胱甘肽S-转移酶(GST)活性保持抑制状态;·OH信号强度与SOD活性和MDA含量的变化具有一致性;SOD活性和MDA含量激活率较高,CAT激活率则保持稳定.
The effects of nano ZnO on the intensity of free radical,antioxidant enzyme activity and malondialdehyde(MDA) content in the liver of Cipangopaludina Cahayensis following 21 d exposure were investigatedto. The Hy-droxyl radical(·OH) concentration was determined by electron paramagnetic resonance(EPR). The results showed that nano ZnO the production of ·OH in the liver of Cipangopaludina Cahayensis; SOD activity and MDA content changed in a similar fashion in that they initially rose then dropped with the concentration of nano ZnO. CAT activity was induced significantly but the change was slow; GST activity remained inhibited. ·OH signal strength was consistent with the change of SOD activity and MDA contents. The activation rate for SOD and MDA was high and CAT and GST activity remained steady.
The effects of nano ZnO on the intensity of free radical,antioxidant enzyme activity and malondialdehyde(MDA) content in the liver of Cipangopaludina Cahayensis following 21 d exposure were investigatedto. The Hy-droxyl radical(·OH) concentration was determined by electron paramagnetic resonance(EPR). The results showed that nano ZnO the production of ·OH in the liver of Cipangopaludina Cahayensis; SOD activity and MDA content changed in a similar fashion in that they initially rose then dropped with the concentration of nano ZnO. CAT activity was induced significantly but the change was slow; GST activity remained inhibited. ·OH signal strength was consistent with the change of SOD activity and MDA contents. The activation rate for SOD and MDA was high and CAT and GST activity remained steady.
引文
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[28]ZHAO X S,WANG S T,WU Y,et al.Acute Zn O nanoparticles exposure induces developmental toxicity,oxidative stress and DNA damage in embryo-larval zebrafish[J].Aquatic Toxicology,2013,136-137:49-59.
[29]ZHANG C,WANG J T,TAN L J,et al.Toxic effects of nano-Zn O on marine microalgae Skeletonema costatum:Attention to the accumulation of intracellular Zn[J].Aquatic Toxicology,2016,178:158-164.
[30]FANG T,YU L P,ZHANG W C,et al.Effects of humic acid and ionic strength on Ti O2nanoparticles sublethal toxicity to zebrafish[J].Ecotoxicology,2015,24:2054-2066.
[31]吴明珠,何梅琳,邹山梅,等.纳米Mg O对斜生栅藻的毒性效应及致毒机理[J].环境化学,2015,34(7):1259-1267.WU M Z,HE M L,ZOU S M,et al.Toxicities and mechanisms of Mg O nanoparticles to Scenedesmus obliquus[J].Environmental Chemistry,2015,34(7):1259-1267(in Chinese).
[2]LI M,LIN D H,ZHU L Z.Effects of water chemistry on the dissolution of Zn O nanoparticles and theirtoxicity to Escherichia coli[J]Environmental Pollution,2013,173:97-102.
[3]SIRELRHATIM A,MAHMUD S,SEENI A,et al.Review on zinc oxide nanoparticles:Antibacterial activity and toxicity mechanism[J]Nano-Micro Letters,2015,7(3):219-242.
[4]SCHIAVO S,OLIVIERO M,MIGLETTA M,et al.Genotoxic and cytotoxic effects of Zn O nanoparticles for Dunaliellatertiolecta and comparison with Si O2and Ti O2effects at populationgrowth inhibition levels[J].Science of the Total Environment,2016,550:619-627.
[5]CHEN X L,O'HALLORAN J,JANSEN M A.The toxicity of zinc oxide nanoparticles to Lemna minor(L.)is predominantly caused b dissolved Zn[J].Aquatic Toxicology,2016,174:46-53.
[6]HASAN K,FATIH A,MEDT G,et al.A comparative toxicity study between small and large size zinc oxide nanoparticles in tilapi(Oreochromis niloticus):Organ pathologies,osmoregulatory responses and immunological parameters[J].Chemosphere,2016,144:571-582.
[7]BESSEMER R A,BUTLER K M,TUNNAH L,et al.Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in th freshwater fish Catostomus commersonii[J].Nanotoxicology,2015,9(7):861-870.
[8]ALI D,ALI H.Susceptibility of the freshwater pulmonate snail Lymnea luteola L.to copper oxide nanoparticl[J].Toxicological and Environmental Chemistry,2015,97(5):576-587.
[9]DAVIES M J,HAVKINS C L.EPR Spin trapping of protein radicals[J].Free Radical Biology and Medicine,2004,36(9):1072-1086
[10]王晓蓉.污染物微观致毒机制和环境生态风险早期诊断[M].北京:科学出版社,2013.WANG X R.Toxic mechanism of pollutants and early diagnosis of ecological risk[M].Beijing:Science Press,2013(in Chinese).
[11]BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dy binding[J].Analytical Biochemistry,1976,72:248-254.
[12]邹国林,桂兴芬,钟晓凌,等.一种SOD的测活方法-邻苯三酚自氧化法的改进[J].生物化学与生物物理学进展,1986,4:71-73.ZOU G L,GUI X F,ZHONG X L,et al.A method to determination of SOD activity-to improve the pyrogallol autoxidation method[J].Progress in Biochemistry and Biophysics,1986,4:71-73(in Chinese).
[13]徐镜波,袁小凡,郎佩贞.过氧化氢酶活性及活性抑制的紫外分光光度法测定[J].环境化学,1997,16(1):73-76.XU J B,YUAN X F,LANG P Z.The determination of enzymic activity and its inhibition of catalase[J].Environmental Chemistry,1997,16(1):73-76(in Chinese).
[14]罗义,施华宏,王晓蓉,等.2,4-二氯苯酚诱导鲫鱼肝脏自由基的产生和脂质过氧化[J].环境科学,2005,26(3):29-32.LUO Y,SHI H H,WANG X R,et al.Free radical generation and lipid peroxidation induced by 2,4-Dichlorophenol in liver of Carassius auratus[J].Environmental Science,2005,26(3):29-32(in Chinese).
[15]HABIG W H,PABST M J,JSKOBY W B.Glutathione-S-transferases the first enzymatic step in mercapturic acid formation[J].Journal of Biological Chemistry,1974,249:7130-7139.
[16]刘慧,朱方伟,尹颖,等.纳米Zn O对鲫鱼肝脏的毒性[J].生态毒理学报,2010,5(5):698-703.LIU H,ZHU F W,YIN Y,et al.Toxicity of nano-Zn O on liver of goldfish(Carassius auratus)[J].Asian Journal of Ecotoxicology,2010,5(5):698-703(in Chinese).
[17]BHUVANESHWARI M,ISWARYA V,ARCHANAA S,et al.Cytotoxicity of Zn O NPs towards fresh water algae Scenedesmus obliquus at low exposure concentrations in UV-C,visibleand dark conditions[J].Aquatic Toxicology,2015,162:29-38.
[18]陈家长,宋超,胡庚东,等.微囊藻毒素-LR对罗非鱼肝脏活性氧自由基含量及相关抗氧化酶活性的影响[J].农业环境科学学报,2011,30(8):1521-1525CHEN J Z,SONG C,HU G D,et al.Effects of microcystin-lr on antioxidant enzymes and reactive oxygen species in tilapia fish[J].Journal of Agro-Environment Science,2011,30(8):1521-1525(in Chinese).
[19]VICARIO-PARES U,CASTANAGA L,LACAVE J M,et al.Comparative toxicity of metal oxide nanoparticles(Cu O,Zn O and Ti O2)to developing zebrafish embryos[J].Journal of Nanoparticle Research,2014,16(8):1-16.
[20]田文静,白伟,赵春禄,等.纳米Zn O对斑马鱼胚胎抗氧化酶系统的影响[J].中国环境科学,2010,30(5):705-709.TIAN W J,BAI W,ZHAO C L,et al.Effects of Zn O nanoparticles on antioxidant enzyme system of zebrafish embryos[J].China Environmental Science,2010,30(5):705-709(in Chinese).
[21]朱方伟.纳米氧化锌对典型水生生物的安全评价[D].南京:河海大学,2010.ZHU F W.Research on safety of nano zinc oxide to modle aquatic[D].Nanjing:Hohai University,2010(in Chinese).
[22]HALINA F,LESYA G,IRINA Y,et al.Reproductive toxicity of inorganic mercury exposure in adultzebrafish:Histological damage,oxidative stress,and alterations of sexhormone and gene expression in the hypothalamic-pituitary-gonadalaxis[J].Aquatic Toxicology,2016,177:417-424.
[23]FALFUSHYNSKA H,GNATYSHYNA L,YURCHAK I,et al.The effects of zinc nanooxide on cellular stress responses of the freshwater mussels Unio tumidus are modulated by elevated temperature and organic pollutants[J].Aquatic Toxicology,2015,162:82-93.
[24]MARISA I,MATOZZO V,MUNARI M,et al.In vivo exposure of the marine clam Ruditapes philippinarumto zinc oxide nanoparticles:responses in gills,digestive glandand haemolymph[J].Environmental Science and Pollution Research,2016,23:15275-15293.
[25]PRAKASH M,GOPALAKRISHNAN N,ILL M C.Alteration in the expression of antioxidant and detoxification genes in Chironomus riparius exposed to zinc oxide nanoparticles[J].Comparative Biochemistry and Physiology,Part B,2015,190:1-7.
[26]PHENNY M,ELIZABETH R CARRAWAY,PETER VAN DEN HURK.The induction of biochemical changes in Daphnia magna by Cu Oand Zn O nanoparticles[J].Aquatic Toxicology,2014,150:201-209.
[27]刘林,赵群芬,朱帅旗,等.纳米氧化锌对斑马鱼肠组织的氧化损伤[J].水产学报,2015,39(11):1702-1711.LIU L,ZHAO Q F,ZHU S Q,et al.Oxidative damage of zinc oxide nanoparticles to zebrafish intestine[J].Journal of Fisheries of China,2015,39(11):1702-1711(in Chinese).
[28]ZHAO X S,WANG S T,WU Y,et al.Acute Zn O nanoparticles exposure induces developmental toxicity,oxidative stress and DNA damage in embryo-larval zebrafish[J].Aquatic Toxicology,2013,136-137:49-59.
[29]ZHANG C,WANG J T,TAN L J,et al.Toxic effects of nano-Zn O on marine microalgae Skeletonema costatum:Attention to the accumulation of intracellular Zn[J].Aquatic Toxicology,2016,178:158-164.
[30]FANG T,YU L P,ZHANG W C,et al.Effects of humic acid and ionic strength on Ti O2nanoparticles sublethal toxicity to zebrafish[J].Ecotoxicology,2015,24:2054-2066.
[31]吴明珠,何梅琳,邹山梅,等.纳米Mg O对斜生栅藻的毒性效应及致毒机理[J].环境化学,2015,34(7):1259-1267.WU M Z,HE M L,ZOU S M,et al.Toxicities and mechanisms of Mg O nanoparticles to Scenedesmus obliquus[J].Environmental Chemistry,2015,34(7):1259-1267(in Chinese).