早期早产儿脐血中S100B蛋白及肌酸激酶脑型同工酶的临床意义
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摘要
目的通过临床检测S100B蛋白、CK-BB判定早产儿脑组织受损情况。方法收集2015年1月-2017年1月在该院妇产科出生的早产儿120例及正常新生儿200例,其中早期早产儿40例,晚期早产儿80例。均取脐带血3 ml离心后取上清液置-70℃冰箱保存待测。应用酶联免疫吸附测定法(ELISA)、双抗体夹心法检测S100B蛋白、CK-BB。结果早产儿与正常组脐血中S100B蛋白、CK-BB比较,差异均无统计学意义(P>0. 05)。早期早产儿与正常组比较,脐血中S100B蛋白差异有统计学意义(P<0. 05);脐血中CK-BB差异无统计学意义(P>0. 05)。晚期早产儿与早期早产儿S100B蛋白、CK-BB比较,差异均无统计学意义(P>0. 05)。结论 100B蛋白在早期早产儿中升高而CK-BB无变化,提示神经胶质细胞首先受到损害,神经元无明显损害。这一部分早产儿是否会发展为脑性瘫痪及对康复治疗的效果有待进一步追踪研究。
Objective To determine damage of brain tissue in premature infants by clinical detection of S100 B protein and CK-BB.Methods From January 2015 to January 2017,120 premature infants and 200 normal neonates born in Department of Gynecology and Obstetrics in the Fifth People's Hospital of Chongqing were collected,40 early premature infants and 80 late premature infants were included.Umbilical cord blood samples (3 ml) were obtained,after centrifugation,supernatant was collected and conserved at-70℃ in refrigerator.ELISA and double-antibody sandwich method were used to detect S100 B protein and CK-BB. Results There was no statistically significant difference in the levels of S100 B protein and CK-BB in umbilical cord blood between premature infants and normal infants (P > 0. 05).There was statistically significant difference in the level of S100 B protein in umbilical cord blood between early premature infants and normal infants (P<0. 05). There was no statistically significant difference in the level of CK-BB in umbilical cord blood between early premature infants and normal infants (P>0. 05). There was no statistically significant difference in the levels of S100 B protein and CK-BB in umbilical cord blood between early premature infants and late premature infants (P>0. 05). Conclusion S100 B protein levels increases in early premature infants,while CK-BB level doesn't change significantly,which indicates that glial cells are the first to be damaged,there is no obvious damage to neurons. These premature infants developing to cerebral palsy and the effect of rehabilitation therapy need to be further followed up and studied.
Objective To determine damage of brain tissue in premature infants by clinical detection of S100 B protein and CK-BB.Methods From January 2015 to January 2017,120 premature infants and 200 normal neonates born in Department of Gynecology and Obstetrics in the Fifth People's Hospital of Chongqing were collected,40 early premature infants and 80 late premature infants were included.Umbilical cord blood samples (3 ml) were obtained,after centrifugation,supernatant was collected and conserved at-70℃ in refrigerator.ELISA and double-antibody sandwich method were used to detect S100 B protein and CK-BB. Results There was no statistically significant difference in the levels of S100 B protein and CK-BB in umbilical cord blood between premature infants and normal infants (P > 0. 05).There was statistically significant difference in the level of S100 B protein in umbilical cord blood between early premature infants and normal infants (P<0. 05). There was no statistically significant difference in the level of CK-BB in umbilical cord blood between early premature infants and normal infants (P>0. 05). There was no statistically significant difference in the levels of S100 B protein and CK-BB in umbilical cord blood between early premature infants and late premature infants (P>0. 05). Conclusion S100 B protein levels increases in early premature infants,while CK-BB level doesn't change significantly,which indicates that glial cells are the first to be damaged,there is no obvious damage to neurons. These premature infants developing to cerebral palsy and the effect of rehabilitation therapy need to be further followed up and studied.
引文
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[18]秋艳萍,焦爱萍,张孝兴,等.抽动障碍儿童血清S100β、CK-BB及GFAP的变化及预后的影响因素分析[J].神经损伤与功能重建,2016,11(6):529-532.
[19]段宝珍,黄应祥,张利.血管内皮生长因子、脑型肌酸激酶同工酶的动态检测在新生儿缺氧缺血性脑病中的意义[J].蚌埠医学院学报,2016,41(6):730-732.
[20]罗菁,方声,王丽珍.缺氧缺血性脑损伤患儿血清NSE和CK-BB的意义[J].中国医刊,2013,48(11):85-86.
[2]龙爱云,瓮占平.S100B蛋白在围产医学中的研究进展[J].医学研究与教育,2013,30(4):75-79.
[3]Doμglas-Escobar M,Weiss MD.Biomarkers of hypoxic-ischemic encephalopathy in newborns[J].Front Neurol,2012,2(3):144-152.
[4]蒋海燕,刘利军,何海英,等.脑白质损伤早产儿血清S100B蛋白、神经元特异度烯醇化酶及肌酸激酶脑型同工酶的变化[J].临床儿科杂志,2013,31(3):221-224.
[5]冯敏,董淮富.S100B蛋白在新生儿缺氧缺血性脑病诊断和预后判断中的研究进展[J].蚌埠医学院学报,2012,37(11):1396-1399.
[6]Beaudeux JL,Laribi S.S100B protein serum level as a biomarker of minor head injury[J].Ann Biol Clin(Paris),2013,71(4):71-78.
[7]Welch RD,Ellis M,Lewis LM,et al.Modeling the kinetics of serum glial fibrillary acidic protein,ubiquitin carboxyl-terminal hydrolase-l1,and s100b concentrations in patients with traumatic brain injury[J].J Neurotrauma,2017,34(11):1957-1971.
[8]Thelin EP,Nelson DW,Bellander BM.A review of the clinical utility of serum S100B protein levels in the assessment of traumatic brain injury[J].Acta Neurochir(Wien),2017,159(2):209-225.
[9]Peng QL,Tao SH,Yu N,et al.Elevated levels of cerebrospinal fluid S100B are associated with brain injury and unfavorable outcomes in children with central nervous system infections[J].Int J Neurosci,2017,127(1):1-9.
[10]Li Y,Yu ZX,Ji MS,et al.A pilot study of the use of dexmedetomidine for the control of delirium by reducing the serum concentrations of brain-derived neurotrophic factor,neuron-specific enolase,and S100B in polytrauma patients[J].J Intensive Care Med,2017[Epub ahead of print].
[11]Esnafoglu E,Ayyildiz SN,Cirrik S,et al.Evaluation of serum neuron-specific enolase,S100B,myelin basic protein and glial fibrilliary acidic protein as brain specific proteins in children with autism spectrum disorder[J].Int J Dev Neurosci,2017,61(1):86-91.
[12]Wu H,Brown EV,Acharya NK,et al.Age-dependent increase of blood-brain barrier permeability and neuron-binding autoantibodies in S100B knockout mice[J].Brain Res,2016,1637(1):154-167.
[13]Celikbilek A,Akyol L,Sabah S,et al.S100B as a glial cell marker in diabetic peripheral neuropathy[J].Neurosci Lett,2013,558(1):53-57.
[14]Carvalho DZ,Sch9nwald SV,Schumacher-Schuh AF,et al.O-vernight S100B in Parkinson's disease:a glimpse into sleep-related neuroinflammation[J].Neurosci Lett,2015,608(1):57-63.
[15]Men XM,Deng B,Tao X,et al.Association analysis of myosin heavy-chain genes mRNA transcription with the corresponding proteins expression of longissimus muscle in growing pigs[J].Asian Aust J Anim Sci,2016,29(4):457-463.
[16]Hottenrott K,Ludyga S,Schulze S,et al.Does a run/walk strategy decrease cardiac stress during a marathon in non-elite runners?[J].J Sci Med Sport,2016,19(1):64-68.
[17]Kotlinska-Hasiec E,Czajkowski M,Rzecki Z,et al.Disturbance in venous outflow from the cerebral circulation intensifies the release of blood-brain barrier injury biomarkers in patients undergoing cardiac surgery[J].J Cardiothorac Vasc Anesth,2014,28(2):328-335.
[18]秋艳萍,焦爱萍,张孝兴,等.抽动障碍儿童血清S100β、CK-BB及GFAP的变化及预后的影响因素分析[J].神经损伤与功能重建,2016,11(6):529-532.
[19]段宝珍,黄应祥,张利.血管内皮生长因子、脑型肌酸激酶同工酶的动态检测在新生儿缺氧缺血性脑病中的意义[J].蚌埠医学院学报,2016,41(6):730-732.
[20]罗菁,方声,王丽珍.缺氧缺血性脑损伤患儿血清NSE和CK-BB的意义[J].中国医刊,2013,48(11):85-86.