同位素标记相对和绝对定量技术筛选的相关差异蛋白在重症肺炎患儿心肌损害和心力衰竭诊断中的临床价值
|
摘要
目的探讨应用同位素标记相对和绝对定量技术(iTRAQ)筛选的相关差异蛋白在重症肺炎患儿并心肌损害和心力衰竭诊断中的临床价值,为临床诊治提供参考依据。方法通过强阳离子交换色谱分析法的8种不同同位素标记的iTRAQ和液相色谱法以及串联质谱分析重症肺炎患儿(观察组)和健康儿童(对照组)血清蛋白。通过Mascot软件分析和GO分析表达差异的蛋白。结果与对照组相比,观察组中共鉴定出有定量信息的蛋白质89个,包括61个上调蛋白和28个下调蛋白。生物信息学分析显示差异表达的蛋白主要参与急性期反应、氧运输活动及酶催化等过程,这些表达差异的蛋白中选择5个显著表达差异作为候选蛋白,分别是CRP、SAA、PI16、BLVRB、G3P。结论本研究发现这5种蛋白在小儿重症肺炎并发心肌损害和心力衰竭过程中发挥重要作用,可能作为该病潜在的临床筛查生物标志物。
Objective We screen the variate proteins from children with severe pneumonia and myocardial injury by i TRAQ technology and probe into their meaning for clinical diagnose. Methods Analyzing the total proteins in serum samples of severe pneumonia children with myocardial damage( observation group) and samples of healthy children( control group) by using eight-plexi TRAQ added with strong cation exchange choematography and liquid chromatography coupled with tandem mass spectrometry. Results A total of 89 differentially expressed proteins were identified,including 68 proteins were up-regulation and 21 proteins were down-regulation. Bioinformatics analysis revealed that differential expression proteins were mainly related to the acute-phase response and oxygen transporter activity,and the catalytic activity of some enzymes process. Among of them,there were 5 proteins significant differentially expressed,including CRP、SAA、PI16、BLVRB、G3P. Conclusion This study found that five kinds of protein in children with severe pneumonia complicated with myocardial injury play an important role in the process,promisingly as the disease clinical screening of potential biomarkers.
Objective We screen the variate proteins from children with severe pneumonia and myocardial injury by i TRAQ technology and probe into their meaning for clinical diagnose. Methods Analyzing the total proteins in serum samples of severe pneumonia children with myocardial damage( observation group) and samples of healthy children( control group) by using eight-plexi TRAQ added with strong cation exchange choematography and liquid chromatography coupled with tandem mass spectrometry. Results A total of 89 differentially expressed proteins were identified,including 68 proteins were up-regulation and 21 proteins were down-regulation. Bioinformatics analysis revealed that differential expression proteins were mainly related to the acute-phase response and oxygen transporter activity,and the catalytic activity of some enzymes process. Among of them,there were 5 proteins significant differentially expressed,including CRP、SAA、PI16、BLVRB、G3P. Conclusion This study found that five kinds of protein in children with severe pneumonia complicated with myocardial injury play an important role in the process,promisingly as the disease clinical screening of potential biomarkers.
引文
[1]廖炀,李秀芬,朱军,等.经鼻持续气道正压通气治疗婴幼儿重症肺炎疗效观察[J].临床儿科杂志,2013,31(2):17-119.
[2]中华医学会儿科学分会呼吸学组,中华儿科杂志编辑委员会.儿童社区获得性肺炎管理指南(2013年修订)[J].中华儿科杂志,2013,51(10):745-752.
[3]VanSickle JS,Srivastava T,Alon US. Life-Threatening Hypercalcemia During Prodrome of Pneumocystis jiroveci Pneumonia in an Immunocompetent Infant[J]. Glob Pediatr Health,2017,2(4):2333794X1770595.
[4]姚金祥,朱雯,滕懿群.重症肺炎肌酶检测临床意义[J].国际呼吸杂志,2012,32(16):1245-1247.
[5]黄彩芝,莫丽亚,杨娟,等.重症肺炎患儿血清N末端脑钠素原和肌钙蛋白I水平变化及意义[J].临床儿科杂志,2014,32(8):724-726.
[6]Meysamie A,Ghodsi S,Ghalehtaki R,et al. Distributions of HighSensitivity C-Reactive Protein, Total Cholesterol-HDL Ratio and10-Year Cardiovascular Risk:National Population-Based Study[J].Acta Med Iran. 2017,55(4):218-227.
[7]Groenveld HF,Januzzi JL,Damman K,et al. Anemia and mortality inheart failure patients:a systematic review and meta-analysis[J].Jam CollCardiol,2008,52(10):818-827.
[8]陈应福.酚妥拉明加多巴胺治疗婴幼儿重症肺炎并发心衰的疗效观察[J].按摩与康复医学旬刊,2011,2(2):131.
[9]Hazell GGJ,Peachey AMG,Teasdale JE,et al. PI16 is a shear stress and inflammation-regulated inhibitor of MMP2[J]. Scientific Reports,2016,6(1):39553.
[10]Zhang YS,He L,Liu B,et al. A novel pathway of NADPH oxidase/vascular peroxidase 1 in mediating oxidative injury follow-ing ischemia-reperfusion[J].Basic Res Cardiol,2012,107(3):266.
[11]付国良,黄晓红.甘油醛-3-磷酸脱氢酶功能的研究进展[J].生物物理学报,2013,29(3):181-191.
[2]中华医学会儿科学分会呼吸学组,中华儿科杂志编辑委员会.儿童社区获得性肺炎管理指南(2013年修订)[J].中华儿科杂志,2013,51(10):745-752.
[3]VanSickle JS,Srivastava T,Alon US. Life-Threatening Hypercalcemia During Prodrome of Pneumocystis jiroveci Pneumonia in an Immunocompetent Infant[J]. Glob Pediatr Health,2017,2(4):2333794X1770595.
[4]姚金祥,朱雯,滕懿群.重症肺炎肌酶检测临床意义[J].国际呼吸杂志,2012,32(16):1245-1247.
[5]黄彩芝,莫丽亚,杨娟,等.重症肺炎患儿血清N末端脑钠素原和肌钙蛋白I水平变化及意义[J].临床儿科杂志,2014,32(8):724-726.
[6]Meysamie A,Ghodsi S,Ghalehtaki R,et al. Distributions of HighSensitivity C-Reactive Protein, Total Cholesterol-HDL Ratio and10-Year Cardiovascular Risk:National Population-Based Study[J].Acta Med Iran. 2017,55(4):218-227.
[7]Groenveld HF,Januzzi JL,Damman K,et al. Anemia and mortality inheart failure patients:a systematic review and meta-analysis[J].Jam CollCardiol,2008,52(10):818-827.
[8]陈应福.酚妥拉明加多巴胺治疗婴幼儿重症肺炎并发心衰的疗效观察[J].按摩与康复医学旬刊,2011,2(2):131.
[9]Hazell GGJ,Peachey AMG,Teasdale JE,et al. PI16 is a shear stress and inflammation-regulated inhibitor of MMP2[J]. Scientific Reports,2016,6(1):39553.
[10]Zhang YS,He L,Liu B,et al. A novel pathway of NADPH oxidase/vascular peroxidase 1 in mediating oxidative injury follow-ing ischemia-reperfusion[J].Basic Res Cardiol,2012,107(3):266.
[11]付国良,黄晓红.甘油醛-3-磷酸脱氢酶功能的研究进展[J].生物物理学报,2013,29(3):181-191.