食品中9种人工合成着色剂的检测
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摘要
本研究在国家标准基础上,采用氨水-70%甲醇(1:99 V/V)为提取溶剂,经弱阴离子固相萃取柱净化、超高效液相色谱分离、紫外检测器分析,通过梯度洗脱和切换波长检测,同时测定了9种食品人工合成色素。本方法检出限0.2 mg/kg,线性范围0.2～10 mg/L,加标回收率85.0%～110.0%,重复性为2.0%～8.9%。
This study was based on the national standard method, and use ammonia-70 % methanol(1:99 V/V) as extract solvent. Sample solution was purified by weak anion solid phase extraction, and finally carried out by the ultra high pressure liquid chromatography with ultraviolet detector. Analyses were separated by gradient elution and measured by the different waves. Nine types synthetic colors in food that could be measured simultaneously. The limitation of determination was 0.2 mg/kg, the range of calibration curves was 0.2～10 mg/L, the range of recoveries was 85.0 %～110.0 % and the repeatability was 2.0 %～8.9 %).
This study was based on the national standard method, and use ammonia-70 % methanol(1:99 V/V) as extract solvent. Sample solution was purified by weak anion solid phase extraction, and finally carried out by the ultra high pressure liquid chromatography with ultraviolet detector. Analyses were separated by gradient elution and measured by the different waves. Nine types synthetic colors in food that could be measured simultaneously. The limitation of determination was 0.2 mg/kg, the range of calibration curves was 0.2～10 mg/L, the range of recoveries was 85.0 %～110.0 % and the repeatability was 2.0 %～8.9 %).
引文
[1]GB 2760-2016,食品安全国家标准食品添加剂使用标准[S].
[2]GB 5009.35-2016,食品安全国家标准食品中人工着色剂的测定[S].
[3]陆琦.食品中人工合成色素检测方法改进[J].粮食与油脂,2010,10期:41-43.
[2]GB 5009.35-2016,食品安全国家标准食品中人工着色剂的测定[S].
[3]陆琦.食品中人工合成色素检测方法改进[J].粮食与油脂,2010,10期:41-43.