正交试验优选刺三加茎、叶中总多酚的提取工艺及含量测定研究
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摘要
以刺三加茎、叶为原料,采用超声波辅助技术提取刺三加茎、叶中总多酚。通过单因素实验和正交试验优选了刺三加茎、叶中提取总多酚类物质的最佳工艺条件。结果表明,刺三加茎中总多酚的最佳提取工艺:乙醇体积分数45%、料液比1∶35(g/m L)、提取时间50 min、提取温度45℃,总多酚平均含量为9. 42 mg/g,提取率平均值为1. 10%;刺三加叶中总多酚最佳提取工艺:乙醇体积分数45%、料液比1∶10(g/m L)、提取时间70 min、提取温度50℃,总多酚平均含量为13. 46mg/g,提取率平均值为1. 35%。刺三加茎、叶中加标平均回收率分别为98. 13%和99. 18%,相对标准偏差(RSD)分别为1. 35%和1. 68%(n=6)。该实验方法简便、可靠、准确,为进一步研究总多酚在实际生产中的应用提供了理论依据。
Using Acanthopanan trifoliatus stems and leaves as raw materials,the total polyphenols in the Acanthopanan trifoliatus stems and leaves were extracted by ultrasonic-assisted extraction. The optimum extraction conditions of total polyphenols from stems and leaves in the Acanthopanan trifoliatus were optimized by single factor experiment and orthogonal test.The results showed that the optimal extraction technology of total polyphenols in Acanthopanan trifoliatus stems was as follows: the volume fraction of ethanol was 45%,the ratio of material to liquid was 1 ∶35( g/m L),the extraction time was 50 min,and the extraction temperature was 45 ℃.Under these conditions,the extraction rate of total polyphenol was the highest.The highest content of total polyphenols was 9. 42 mg/g,and the average extraction rate of total polyphenols was 1. 10%.The optimum extraction technology of total polyphenols in Acanthopanan trifoliatus leaves was as follows: the volume fraction of ethanol was 45%,the ratio of material to liquid was 1 ∶10( g/m L),the extraction time was 70 min,and the extraction temperature was 50 ℃.The highest content of total polyphenols was 13. 46 mg/g,and the average extraction rate of total polyphenols was 1. 35%.The average recoveries were 98. 13% and99. 18%,and the RSD( n = 6) were 1. 35% and 1. 68%,respectively.The method was simple,accurate and reliable.It was suitable for the determination of total polyphenols in the stems and leaves of Acanthopanan trifoliatus. Therefore,it provided a theoretical basis for further research on the application of total polyphenols in practical production.
Using Acanthopanan trifoliatus stems and leaves as raw materials,the total polyphenols in the Acanthopanan trifoliatus stems and leaves were extracted by ultrasonic-assisted extraction. The optimum extraction conditions of total polyphenols from stems and leaves in the Acanthopanan trifoliatus were optimized by single factor experiment and orthogonal test.The results showed that the optimal extraction technology of total polyphenols in Acanthopanan trifoliatus stems was as follows: the volume fraction of ethanol was 45%,the ratio of material to liquid was 1 ∶35( g/m L),the extraction time was 50 min,and the extraction temperature was 45 ℃.Under these conditions,the extraction rate of total polyphenol was the highest.The highest content of total polyphenols was 9. 42 mg/g,and the average extraction rate of total polyphenols was 1. 10%.The optimum extraction technology of total polyphenols in Acanthopanan trifoliatus leaves was as follows: the volume fraction of ethanol was 45%,the ratio of material to liquid was 1 ∶10( g/m L),the extraction time was 70 min,and the extraction temperature was 50 ℃.The highest content of total polyphenols was 13. 46 mg/g,and the average extraction rate of total polyphenols was 1. 35%.The average recoveries were 98. 13% and99. 18%,and the RSD( n = 6) were 1. 35% and 1. 68%,respectively.The method was simple,accurate and reliable.It was suitable for the determination of total polyphenols in the stems and leaves of Acanthopanan trifoliatus. Therefore,it provided a theoretical basis for further research on the application of total polyphenols in practical production.
引文
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[14]郭蒙,高林晓,李云萍,等.UV-Vis分光光度法测刺三加叶中总多酚[J].食品工业,2018,39(6):291-295.
[15]黎代余,黄丽凤,陈鸿平,等.不同产地大花红景天有效成分的含量测定研究[J].食品工业科技,2011,32(2):347-349.
[16]彭茹洁,汪佳丹,韩伟.植物多酚提取、分离纯化及其分析方法的研究进展[J].机电信息,2016,(14):21-29.
[17]阮冲,肖小华,李攻科.天然产物有效成分提取分离制备方法研究进展[J].化学试剂,2014,36(3):193-200.
[18]斯琴格日乐,恩德.茶叶有效成分茶多酚的提取工艺研究[J].河南理工大学学报(自然科学版),2011,30(5):625-628.
[19]肖凤艳,孙立彬.生地黄总多酚超声法提取工艺研究[J].江苏农业科学,2012,40(11):294-295.
[20]林志銮,王欢,赵春天,等.超声波辅助提取仙鹤草多酚工艺研究[J].昆明学院学报,2016,38(3):66-69.
[2]ANDLAUER W,STEHLE P,FURST P.Chemoprevention---a novel approach in dietetics[J].Curr.Opin.Clin.Nutr.,1998,1(6):539-547.
[3]NEGI P S,JAYAPRASHA G K,JENA B S.Antioxidant and anti-mutagenic activities of pomegranate peel extracts[J].Food Chem.,2003,80(3):393-397.
[4]PARK S Y,CHANG S Y,OH O J,et al.Nor-oleanene type triterpene glycosides from the leaves of Acanthopanax japonicus[J].Phytochemistry,2002,59(4):379-384.
[5]杨贤强,曹明富,沈生荣,等.茶多酚生物学活性的研究[J].茶叶科学,1993,13(1):51-59.
[6]谭悦,王晓静,陈光静,等.木瓜酒发酵过程中多酚类物质的高效液相色谱测定及含量变化分析[J].食品与发酵工业,2018,44(2):209-216.
[7]郭会琴,熊晶晶,颜流水,等.高效液相色谱-质谱法测定油茶果壳中5种多酚类化合物的含量[J].理化检验-化学分册,2015,51(11):1 493-1 496.
[8]高林晓,郭蒙,刘凤华,等.超声浸提-高效液相色谱法测定刺三加根和茎中没食子酸含量[J].中国食品添加剂,2018,(6):145-150.
[9]杨新周,郭云胶,田孟华.硬巢大须球白蚁巢黄酮、多酚的同步提取工艺优化及抗氧化活性[J].化学试剂,2018,40(11):1 102-1 107.
[10]张娅,石方剑,杨有仙.福林-酚比色法测定葡萄烈酒中总多酚含量的研究[J].中国酿造,2017,36(10):163-166.
[11]王烨军,徐亦鼎,黄建琴,等.酒石酸亚铁比色法和高锰酸钾滴定法测定茶多酚的比较[J].茶业通报,2010,32(2):61-63.
[12]张素斌,李晔,张国境.田基黄多酚的提取及其抗氧化活性的研究[J].中国食品添加剂,2015,(6):86-91.
[13]钟益宁,吴诗云,张焱,等.肉桂枝叶非挥发部分总多酚测定及其体外抗菌作用[J].中成药,2016,38(10):2 297-2 300.
[14]郭蒙,高林晓,李云萍,等.UV-Vis分光光度法测刺三加叶中总多酚[J].食品工业,2018,39(6):291-295.
[15]黎代余,黄丽凤,陈鸿平,等.不同产地大花红景天有效成分的含量测定研究[J].食品工业科技,2011,32(2):347-349.
[16]彭茹洁,汪佳丹,韩伟.植物多酚提取、分离纯化及其分析方法的研究进展[J].机电信息,2016,(14):21-29.
[17]阮冲,肖小华,李攻科.天然产物有效成分提取分离制备方法研究进展[J].化学试剂,2014,36(3):193-200.
[18]斯琴格日乐,恩德.茶叶有效成分茶多酚的提取工艺研究[J].河南理工大学学报(自然科学版),2011,30(5):625-628.
[19]肖凤艳,孙立彬.生地黄总多酚超声法提取工艺研究[J].江苏农业科学,2012,40(11):294-295.
[20]林志銮,王欢,赵春天,等.超声波辅助提取仙鹤草多酚工艺研究[J].昆明学院学报,2016,38(3):66-69.