摘要
以刺三加茎、叶为原料,采用超声波辅助技术提取刺三加茎、叶中总多酚。通过单因素实验和正交试验优选了刺三加茎、叶中提取总多酚类物质的最佳工艺条件。结果表明,刺三加茎中总多酚的最佳提取工艺:乙醇体积分数45%、料液比1∶35(g/m L)、提取时间50 min、提取温度45℃,总多酚平均含量为9. 42 mg/g,提取率平均值为1. 10%;刺三加叶中总多酚最佳提取工艺:乙醇体积分数45%、料液比1∶10(g/m L)、提取时间70 min、提取温度50℃,总多酚平均含量为13. 46mg/g,提取率平均值为1. 35%。刺三加茎、叶中加标平均回收率分别为98. 13%和99. 18%,相对标准偏差(RSD)分别为1. 35%和1. 68%(n=6)。该实验方法简便、可靠、准确,为进一步研究总多酚在实际生产中的应用提供了理论依据。
Using Acanthopanan trifoliatus stems and leaves as raw materials,the total polyphenols in the Acanthopanan trifoliatus stems and leaves were extracted by ultrasonic-assisted extraction. The optimum extraction conditions of total polyphenols from stems and leaves in the Acanthopanan trifoliatus were optimized by single factor experiment and orthogonal test.The results showed that the optimal extraction technology of total polyphenols in Acanthopanan trifoliatus stems was as follows: the volume fraction of ethanol was 45%,the ratio of material to liquid was 1 ∶35( g/m L),the extraction time was 50 min,and the extraction temperature was 45 ℃.Under these conditions,the extraction rate of total polyphenol was the highest.The highest content of total polyphenols was 9. 42 mg/g,and the average extraction rate of total polyphenols was 1. 10%.The optimum extraction technology of total polyphenols in Acanthopanan trifoliatus leaves was as follows: the volume fraction of ethanol was 45%,the ratio of material to liquid was 1 ∶10( g/m L),the extraction time was 70 min,and the extraction temperature was 50 ℃.The highest content of total polyphenols was 13. 46 mg/g,and the average extraction rate of total polyphenols was 1. 35%.The average recoveries were 98. 13% and99. 18%,and the RSD( n = 6) were 1. 35% and 1. 68%,respectively.The method was simple,accurate and reliable.It was suitable for the determination of total polyphenols in the stems and leaves of Acanthopanan trifoliatus. Therefore,it provided a theoretical basis for further research on the application of total polyphenols in practical production.
引文
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