乌苏里蝮罹患间叶型软骨肉瘤研究
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摘要
在人工养殖中,乌苏里蝮健康情况值得关注。为了探究病情,并为以后有效地预防和治疗提供资料,本研究室对养殖种群进行了筛查。针对其中颈部有异常肿大的个体进行了血液学检测、组织学观察和免疫组化鉴定。观察结果显示:患病蛇脂肪体系数显著大于正常蛇;白细胞和血栓细胞总数显著低于正常蛇,糖链抗原24-2呈阳性。肿块的组织学切片及免疫组化染色结果中可见典型的上皮细胞肿瘤的特征结构——癌穴,常规用于鉴定肿瘤的标记物S100、VIM、EMA和GFAP均呈阳性,故确定该肿大物为间叶型软骨肉瘤,但诱发原因还需要进一步研讨。建议在人工繁育乌苏里蝮的过程中,需要精选种蛇,并对养殖种群进行定期的筛选,提高其健康程度。
In farming,the health of Gloydius ussuriensis is a concern. We screened the breeding population to assess the status of disease among farmed Wusuli pit vipers to enhance disease prevention and treatment. An individual with abnormal swelling of the neck was examined by hematology,histology and immunohistochemistry. The results showed that the fat coefficient of suffering G. ussuriensis was significantly larger than that of uninfected snakes. The number of white blood cells and thrombus cells was significantly lower in infected snakes than in uninfected ones. Serologically tested tumor markers for carbohydrate antigen CA 24-2 were positive. Characteristic structures of typical epithelial cell tumors were detected by use of histological and immunohistochemical methods. The markers( S100,VIM,EMA and GFAP) for tumor identification were positive,enabling confirmation of Chondrosarcoma. The cause of inducement was not determined.We recommend that in captive breeding of G. ussuriensis,breeder snakes be selected and the breeding population should be regularly screened for its health.
In farming,the health of Gloydius ussuriensis is a concern. We screened the breeding population to assess the status of disease among farmed Wusuli pit vipers to enhance disease prevention and treatment. An individual with abnormal swelling of the neck was examined by hematology,histology and immunohistochemistry. The results showed that the fat coefficient of suffering G. ussuriensis was significantly larger than that of uninfected snakes. The number of white blood cells and thrombus cells was significantly lower in infected snakes than in uninfected ones. Serologically tested tumor markers for carbohydrate antigen CA 24-2 were positive. Characteristic structures of typical epithelial cell tumors were detected by use of histological and immunohistochemical methods. The markers( S100,VIM,EMA and GFAP) for tumor identification were positive,enabling confirmation of Chondrosarcoma. The cause of inducement was not determined.We recommend that in captive breeding of G. ussuriensis,breeder snakes be selected and the breeding population should be regularly screened for its health.
引文
[1] SYKES J M,TRUPKIEWICZ J G.Reptile neoplasia at the Philadelphia Zoological Garden,1901-2002[J].Journal of Zoo and Wildlife Medicine,2006,37(1):11-19.
[2] GARNER M M,HERNANDEZ-DIVERS S M,RAYMOND J T.Reptile neoplasia:a retrospective study of case submissions to a specialty diagnostic service[J].The Veterinary Clinics of North America.Exotic Animal Practice,2004,7(3):653-671.
[3] 张冰,陶思源,王珊珊,等.人工养殖棕黑锦蛇体表寄生螨的治疗初探[J].经济动物学报,2015,19(3):147-151.
[4] 于宏珠,程建鑫,张冰,等.人工饲养条件下棕黑锦蛇肾脏病变的初步观察[J].经济动物学报,2016,20(2):94-98.
[5] SCHMIDT R E,REAVILL D R.Metastatic chondrosarcoma in a corn snake(Pantherophis guttatus)[J].Journal of Herpetological Medicine and Surgery,2012,22(3/4):67-69.
[6] 姚忠祎,陆宇燕,李丕鹏.乌苏里蝮生殖系统的形态结构和发育的初步研究[J].经济动物学报,2015,19(1):10-15.
[7] KORTEN A G G C,TER BERG H J W,SPINCEMAILLE G H,et al.Intracranial chondrosarcoma:review of the literature and report of 15 cases[J].Journal of Neurology,Neurosurgery & Psychiatry,1998,65(1):88-92.
[8] 李非田,戴春富.颈静脉孔区软骨肉瘤的诊断与治疗[J].临床耳鼻咽喉头颈外科杂志,2017,31(18):1415-1418.
[9] 杨文博.间叶性软骨肉瘤156例文献综合分析[J].中国实用内科杂志,2016,36(增刊2):214-215.
[10] 董健文,郭卫,燕太强,等.多发性骨外软骨肉瘤一例[J].中国骨肿瘤骨病,2003,2(1):63-64;62.
[11] SCH?FER B W,HEIZMANN C W.The S100 family of EF-hand calcium-binding proteins:functions and pathology[J].Trends in Biochemical Sciences,1996,21(4):134-140.
[12] 林勇,叶建新,林旭.波形蛋白表达与上皮源性肿瘤的关系[J].医学综述,2010,16(1):50-52.
[13] 王正位,王磊,单锋芝,等.肾脏间叶性软骨肉瘤一例并文献复习[J].中华肿瘤防治杂志,2015,22(19):1578-1579.
[14] 何振,徐勇,张志宏,等.前列腺恶性间叶性软骨肉瘤1例并文献复习[J].山东医药,2015,55(48):77-79.
[15] 杨岳.STIP1、EMA和ER-α蛋白在甲状腺乳头状癌中的表达及其意义[D].石家庄:河北医科大学,2015.
[16] 曹鹏.人脑胶质原纤维酸性蛋白(GFAP)原核表达载体的构建及蛋白表达的鉴定[D].上海:第二军医大学,2009.
[17] 仲金秋,曹玉珠,沈培亮,等.血小板RNA与肿瘤[J].中国药理学通报,2017,33(4):461-464.
[18] 范永飞,曾慧娟,王少华.血小板在恶性肿瘤转移中的作用研究进展[J].医学研究生学报,2017,30(11):1227-1232.
[19] 常绪祥.直肠癌患者血小板增高的临床意义[D].泰安:泰山医学院,2014.
[20] 王石莹,闫素梅.碱性磷酸酶在动物骨骼代谢中的研究进展[J].饲料博览,2009(4):14-17.
[21] 张庆莲,张青云.乳酸脱氢酶同工酶在肿瘤诊断中的临床意义[J].中华临床实验室管理电子杂志,2015,3(2):82-86.
[22] 王丽娜,宋艳荣,石峻.羟丁酸脱氢酶/乳酸脱氢酶在心肌损伤与肝脏损伤中的鉴别诊断价值[J].中国综合临床,2006,22(2):128-129.
[2] GARNER M M,HERNANDEZ-DIVERS S M,RAYMOND J T.Reptile neoplasia:a retrospective study of case submissions to a specialty diagnostic service[J].The Veterinary Clinics of North America.Exotic Animal Practice,2004,7(3):653-671.
[3] 张冰,陶思源,王珊珊,等.人工养殖棕黑锦蛇体表寄生螨的治疗初探[J].经济动物学报,2015,19(3):147-151.
[4] 于宏珠,程建鑫,张冰,等.人工饲养条件下棕黑锦蛇肾脏病变的初步观察[J].经济动物学报,2016,20(2):94-98.
[5] SCHMIDT R E,REAVILL D R.Metastatic chondrosarcoma in a corn snake(Pantherophis guttatus)[J].Journal of Herpetological Medicine and Surgery,2012,22(3/4):67-69.
[6] 姚忠祎,陆宇燕,李丕鹏.乌苏里蝮生殖系统的形态结构和发育的初步研究[J].经济动物学报,2015,19(1):10-15.
[7] KORTEN A G G C,TER BERG H J W,SPINCEMAILLE G H,et al.Intracranial chondrosarcoma:review of the literature and report of 15 cases[J].Journal of Neurology,Neurosurgery & Psychiatry,1998,65(1):88-92.
[8] 李非田,戴春富.颈静脉孔区软骨肉瘤的诊断与治疗[J].临床耳鼻咽喉头颈外科杂志,2017,31(18):1415-1418.
[9] 杨文博.间叶性软骨肉瘤156例文献综合分析[J].中国实用内科杂志,2016,36(增刊2):214-215.
[10] 董健文,郭卫,燕太强,等.多发性骨外软骨肉瘤一例[J].中国骨肿瘤骨病,2003,2(1):63-64;62.
[11] SCH?FER B W,HEIZMANN C W.The S100 family of EF-hand calcium-binding proteins:functions and pathology[J].Trends in Biochemical Sciences,1996,21(4):134-140.
[12] 林勇,叶建新,林旭.波形蛋白表达与上皮源性肿瘤的关系[J].医学综述,2010,16(1):50-52.
[13] 王正位,王磊,单锋芝,等.肾脏间叶性软骨肉瘤一例并文献复习[J].中华肿瘤防治杂志,2015,22(19):1578-1579.
[14] 何振,徐勇,张志宏,等.前列腺恶性间叶性软骨肉瘤1例并文献复习[J].山东医药,2015,55(48):77-79.
[15] 杨岳.STIP1、EMA和ER-α蛋白在甲状腺乳头状癌中的表达及其意义[D].石家庄:河北医科大学,2015.
[16] 曹鹏.人脑胶质原纤维酸性蛋白(GFAP)原核表达载体的构建及蛋白表达的鉴定[D].上海:第二军医大学,2009.
[17] 仲金秋,曹玉珠,沈培亮,等.血小板RNA与肿瘤[J].中国药理学通报,2017,33(4):461-464.
[18] 范永飞,曾慧娟,王少华.血小板在恶性肿瘤转移中的作用研究进展[J].医学研究生学报,2017,30(11):1227-1232.
[19] 常绪祥.直肠癌患者血小板增高的临床意义[D].泰安:泰山医学院,2014.
[20] 王石莹,闫素梅.碱性磷酸酶在动物骨骼代谢中的研究进展[J].饲料博览,2009(4):14-17.
[21] 张庆莲,张青云.乳酸脱氢酶同工酶在肿瘤诊断中的临床意义[J].中华临床实验室管理电子杂志,2015,3(2):82-86.
[22] 王丽娜,宋艳荣,石峻.羟丁酸脱氢酶/乳酸脱氢酶在心肌损伤与肝脏损伤中的鉴别诊断价值[J].中国综合临床,2006,22(2):128-129.