脾气虚型兔视网膜脱离自动复位后视网膜电图的研究
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摘要
目的观察脾气虚型兔孔源性视网膜脱离(RRD)自动复位后视网膜电图的变化,评价脾气虚证对RRD复位后视网膜功能恢复的影响。方法 21只健康成年新西兰灰兔随机分为空白对照组(A组)、RRD自动复位组(B组)、脾气虚型RRD自动复位组(C组),采用耗气破气加饥饱失常法建立脾气虚证动物模型,在显微镜下行视网膜下注射透明质酸钠术建立视网膜脱离自动复位动物模型,分别于模型制备后10 d、20 d、30 d行视网膜电图(ERG)检查并进行分析比较。结果观察期间,C组兔脾气虚证持续存在,视网膜下注射透明质酸钠术后5～9 d,B组及C组视网膜自动复位。与A组比较,C组术后暗视ERG b波潜伏期及振幅,a波潜伏期,∑OPs振幅等检测值均有显著差异,表现为潜伏期延长及振幅降低,在术后30 d,B组与A组以上各值已无统计学差异,C组仍与A组比较有统计学差异。结论脾气虚证这一病理状态对RRD复位后Müller细胞和双极细胞、无长突细胞的功能恢复产生更为显著的不良影响,而且涉及视锥细胞和视杆细胞。
[Abstraction] OBJECTIVE To explore functional electroretinogram(ERG) changes following experimental retinal reattachment in a spleen-qi deficiency rabbit model. METHODS 21 pigment rabbits were randomly divided into normal control group(group A), retinal automatically reattachment group(group B), and retinal automatically reattachment with spleen-qi deficiency syndrome group(group C). The models of spleen-qi deficiency were induced by exhausting and breaking the spleen-qi as well as irregular feed. The retinal automatically reattachment models were prepared by injecting sodium halyuronate(Helen) into subretinal spaces. Electroretinograms(ERGs)were recorded at the beginning of the study and 10 d, 20 d, and 30 d after modeling respectively.RESULTS The syndrome of spleen-qi deficiency presented persistently in group C during the whole observation. Retinal reattachment occurred from the 5 th day to the 9 th day after remodeling.Compared with group B, the amplitude of rod response, single flash response, 30 Hz flicker response,∑Ops of scotopic vision decreased obviously and the latency of rod response delayed obviously(P<0.05). CONCLUSIONS The results suggested that the pathology of spleen qi syndromebrought an adverse effect to the functional recovery of Müller cells, bipolar cells and amacrine cells following retinal reattachment, moreover it also involved both cone cells and rod cells.
[Abstraction] OBJECTIVE To explore functional electroretinogram(ERG) changes following experimental retinal reattachment in a spleen-qi deficiency rabbit model. METHODS 21 pigment rabbits were randomly divided into normal control group(group A), retinal automatically reattachment group(group B), and retinal automatically reattachment with spleen-qi deficiency syndrome group(group C). The models of spleen-qi deficiency were induced by exhausting and breaking the spleen-qi as well as irregular feed. The retinal automatically reattachment models were prepared by injecting sodium halyuronate(Helen) into subretinal spaces. Electroretinograms(ERGs)were recorded at the beginning of the study and 10 d, 20 d, and 30 d after modeling respectively.RESULTS The syndrome of spleen-qi deficiency presented persistently in group C during the whole observation. Retinal reattachment occurred from the 5 th day to the 9 th day after remodeling.Compared with group B, the amplitude of rod response, single flash response, 30 Hz flicker response,∑Ops of scotopic vision decreased obviously and the latency of rod response delayed obviously(P<0.05). CONCLUSIONS The results suggested that the pathology of spleen qi syndromebrought an adverse effect to the functional recovery of Müller cells, bipolar cells and amacrine cells following retinal reattachment, moreover it also involved both cone cells and rod cells.
引文
[1] FISHER S K, LEWIS G P, LINBERG K A, et al. Cellular remodeling in mammalian retina:results from studies of experimental retinal detachment[J].Progress in Retinal and Eye Research,2005,24(3):395-431.
[2] SOUZA C F D, KALLONIATIS M, POLKINGHORNE P J, et al.Functional and anatomical remodeling in human retinal detachment[J].Experimental Eye Research,2012,97(1):73-89.
[3] BARLIYA T, OFRI R, SANDALON S, et al. Changes in retinal function and cellular remodeling following experimental retinal detachment in a rabbit model[J]. Journal of Ophthalmology,2017,2017(3):14.
[4]张妍春,王卉芳,毕春潮,等.脾气虚型兔视网膜脱离自动复位后视网膜组织形态学观察[J].中国中医基础医学杂志,2011,17(03):267-269.
[5]张妍春,毕春潮,杨新光,等.脾气虚型兔视网膜脱离复位后视细胞超微结构改变的实验研究[J].中国中医眼科杂志,2009,19(2):63-66.
[6]张妍春,陈明霞,毕春潮,等.脾虚气弱对兔视网膜脱离复位后双极细胞及节细胞变化的影响[J].中国中医眼科杂志,2009,19(6):323-326.
[7]陈小野.实用中医证侯动物模型学[M].北京:北京医科大学中国协和医科大学联合出版社,1993:184-189.
[8]王卉芳,张妍春,朱赛林,等.实验性网脱复位后未脱离区域视网膜组织形态学观察[J].陕西医学杂志,2006,35(8):954-956.
[9] LEWIS G P, FISHER S K. Retinal Plasticity and Interactive Cellular Remodeling in Retinal Detachment and Reattachment[M]//Pinaud R,Tremere LA,De Weerd P.Plasticity in the Visual System:From Genes to Circuits, Boston,MA:Springer US,2006,55-78.
[10] SAKAI T, TSUNEOKA H, LEWIS G P, et al. Remodelling of retinal on-and off-bipolar cells following experimental retinal detachment[J].Clin Exp Ophthalmol,2014,42(5):480-485.
[11] KIM I T, HA S M, YOON K C. Electroretinographic studies in rhegmatogenous retinal detachment before and after reattachment surgery[J].Korean J Ophthalmol,2002,15(2):118-127.
[12]孙晓东,张皙,吴星伟,等.视网膜脱离复位后的视网膜电图研究[J].临床眼科杂志,2000,8(6):405-407.
[13]张妍春,雷春灵,杨新光,等.益气养阴利水剂对脾气虚型视网膜脱离复位后抗凋亡及抗氧化作用的实验研究[J].中国中医基础医学杂志,2015,21(2):153-158.
[2] SOUZA C F D, KALLONIATIS M, POLKINGHORNE P J, et al.Functional and anatomical remodeling in human retinal detachment[J].Experimental Eye Research,2012,97(1):73-89.
[3] BARLIYA T, OFRI R, SANDALON S, et al. Changes in retinal function and cellular remodeling following experimental retinal detachment in a rabbit model[J]. Journal of Ophthalmology,2017,2017(3):14.
[4]张妍春,王卉芳,毕春潮,等.脾气虚型兔视网膜脱离自动复位后视网膜组织形态学观察[J].中国中医基础医学杂志,2011,17(03):267-269.
[5]张妍春,毕春潮,杨新光,等.脾气虚型兔视网膜脱离复位后视细胞超微结构改变的实验研究[J].中国中医眼科杂志,2009,19(2):63-66.
[6]张妍春,陈明霞,毕春潮,等.脾虚气弱对兔视网膜脱离复位后双极细胞及节细胞变化的影响[J].中国中医眼科杂志,2009,19(6):323-326.
[7]陈小野.实用中医证侯动物模型学[M].北京:北京医科大学中国协和医科大学联合出版社,1993:184-189.
[8]王卉芳,张妍春,朱赛林,等.实验性网脱复位后未脱离区域视网膜组织形态学观察[J].陕西医学杂志,2006,35(8):954-956.
[9] LEWIS G P, FISHER S K. Retinal Plasticity and Interactive Cellular Remodeling in Retinal Detachment and Reattachment[M]//Pinaud R,Tremere LA,De Weerd P.Plasticity in the Visual System:From Genes to Circuits, Boston,MA:Springer US,2006,55-78.
[10] SAKAI T, TSUNEOKA H, LEWIS G P, et al. Remodelling of retinal on-and off-bipolar cells following experimental retinal detachment[J].Clin Exp Ophthalmol,2014,42(5):480-485.
[11] KIM I T, HA S M, YOON K C. Electroretinographic studies in rhegmatogenous retinal detachment before and after reattachment surgery[J].Korean J Ophthalmol,2002,15(2):118-127.
[12]孙晓东,张皙,吴星伟,等.视网膜脱离复位后的视网膜电图研究[J].临床眼科杂志,2000,8(6):405-407.
[13]张妍春,雷春灵,杨新光,等.益气养阴利水剂对脾气虚型视网膜脱离复位后抗凋亡及抗氧化作用的实验研究[J].中国中医基础医学杂志,2015,21(2):153-158.